A method for enzymatically degrading and extracting active ingredients from fresh velvet antler

A technology of active ingredients and velvet antler enzyme, which is applied in the field of extracting active ingredients by directly degrading fresh deer antler by Alcalase alkaline protease, can solve the problems of incomplete active ingredients, large equipment investment cost, complicated operation, etc., and achieves improved application value and economic benefits. , to solve the shortage of supply, the effect of mild reaction conditions

Inactive Publication Date: 2011-12-21
CHANGCHUN UNIV OF TECH
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Problems solved by technology

(3) Supercritical CO 2 Extraction [8-10] : Supercritical CO 2 The extraction technology is a purely physical way to extract the active substances in velvet antler, which has the characteristics of non-toxic, environmental protection, short extraction time and high product quality, but the extraction rate of active substances in...

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  • A method for enzymatically degrading and extracting active ingredients from fresh velvet antler
  • A method for enzymatically degrading and extracting active ingredients from fresh velvet antler
  • A method for enzymatically degrading and extracting active ingredients from fresh velvet antler

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Embodiment 1

[0019] Embodiment 1: technical scheme of the present invention comprises the following operation steps:

[0020] (1) Fresh velvet antler is singeed, washed, and cut into slices mechanically at 4-6°C;

[0021] (2) Take 15.01g fresh velvet antler cut into flakes, put it into a 500mL beaker, add sodium lactate-sodium hydroxide buffer solution according to the ratio of substrate concentration of 0.1g / mL, grind it to micron level with a colloid mill, and adjust the pH value is 8.5, adding Alcalase alkaline protease at 0.02 times the weight of fresh velvet antler cut into flakes, degrading at 65°C for 6 hours, keeping the pH value constant during the degradation process, heating at 95°C for 15 minutes after degradation, and alkaline Alcalase Protease inactivation; the buffer solution used is NH with a pH value of 9-12 3 .H 2 O;

[0022] (3) Use filter paper or centrifugal filtration to remove the degraded residue of fresh velvet antler, collect the filtrate, distill the obtained ...

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Abstract

The invention provides a method for extracting active ingredients of fresh pilos antler through enzymatic degradation. The fresh pilos antler is treated through Alcalase alkali protease degradation, the biologic active substance concentrated solution of the pilos antler is obtained, and the degradation rate is 82 percent to 93 percent; the obtained biologic active substance concentrated solution of the pilos antler contains various active ingredients such as fatty acid, phospholipids, steroid, glucide, trace elements and the like, all organic substances become a solution state easy for human body to absorb, and the active ingredients can be used for various aspects such as pharmacy, health care products, food, cosmetics and the like; the Alcalase alkali protease is used for degrading the fresh pilos antler, and residues after fresh pilos antler degradation are pilos antler calcification materials, inorganic elements and the like and can be used as good feed additives; after the separation and purification of the biologic active substance concentrated solution of the pilos antler, the obtained amino acid products with the purity being 97 percent are used as important nourishments, and the amino acid yield reaches more than 12 percent. The method has the advantages that the reaction condition is mild, the operation is simple, all the active ingredients of the pilos antler can be fully utilized, and the application value and the economic benefit of the pilos antler are improved.

Description

technical field [0001] The invention belongs to a method for enzymatically degrading and extracting active components from fresh velvet antler, and relates to a method for directly degrading fresh velvet antler and extracting active components by using Alcalase alkaline protease. Background technique: [0002] Antler refers to the unossified horn tissue of male sika deer or red deer. It is a famous traditional Chinese medicinal material, containing phospholipids, glycolipids, gums, hormones, fatty acids, amino acids, vitamins, proteins, calcium, phosphorus, magnesium, sodium, etc. [1] ingredients. In recent years, some active substances have been isolated by freeze-drying method, and velvet antler contains a variety of physiologically active ingredients [2] , such as nerve growth factor, epidermal growth factor, insulin-like growth factor, transforming growth factor and velvet polypeptide. According to their solubility, they are divided into water-soluble, fat-soluble, and...

Claims

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Application Information

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IPC IPC(8): C12P13/04
Inventor 张龙王兰兰
Owner CHANGCHUN UNIV OF TECH
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