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Preservative for fetus free DNA in peripheral blood of pregnant woman and vacuum blood sampling tube composed of preservative

A technology for pregnant women's peripheral blood and preservatives, which is applied in the direction of cell culture active agents, blood/immune system cells, specific-purpose bioreactors/fermenters, etc., and can solve problems such as easy degradation and short storage time of fetal free DNA. To achieve the effect of inhibiting DNase-mediated degradation, preventing the release of cell chromosomal DNA, and preventing the impact of sample plasma

Active Publication Date: 2016-12-21
CHENGDU RICH SCI IND
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: there is no collection and storage technology for the in vitro detection of free DNA in the peripheral blood of mature pregnant women, which leads to the short storage time and easy degradation of fetal free DNA in the existing plasma. A preservative for fetal cell-free DNA in the peripheral blood of pregnant women and the vacuum blood collection tube it consists of

Method used

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  • Preservative for fetus free DNA in peripheral blood of pregnant woman and vacuum blood sampling tube composed of preservative
  • Preservative for fetus free DNA in peripheral blood of pregnant woman and vacuum blood sampling tube composed of preservative
  • Preservative for fetus free DNA in peripheral blood of pregnant woman and vacuum blood sampling tube composed of preservative

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Embodiment 1

[0026] A preservation agent for fetal free DNA in the peripheral blood of pregnant women, including cell stabilizers, anticoagulants, buffers, metabolic inhibitors and DNase inhibitors.

[0027] In the present embodiment, the cell stabilizer is diazolidinyl urea and NaF, the anticoagulant is EDTA potassium salt, the buffer is a standard Tris-Cl buffer with a final concentration of 10 mM, and the metabolic inhibitor is molecular biological Grade glycogen, the DNase inhibitor is dithiothreitol (DTT).

[0028] Wherein, the proportioning ratio of each component is specifically set as shown in Table 1.

[0029] Table 1

[0030]

Embodiment 2

[0032] The difference between this embodiment and embodiment 1 is that in this embodiment, NaF in embodiment 1 is replaced by formaldehyde with a concentration of 40% as example 4-6, and the specific settings are as follows:

example 4

[0033] Example 4: The NaF in Example 1 was replaced by 40% formaldehyde in an amount of 30 μl;

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Abstract

The invention discloses a preservative for fetus free DNA in peripheral blood of pregnant woman and a vacuum blood sampling tube composed of the preservative. The problems of short storage time and easiness in degrading of the fetus free DNA in plasma caused by the shortage of mature collecting and storing technique for the in vitro test of the fetus free DNA in peripheral blood of pregnant woman are solved. The preservative provided by the invention comprises a cell stabilizing agent, an anti-coagulant agent, a buffering solution, a metabolic inhibitor and a DNA enzyme inhibitor. The preservative can stabilize the karyocyte of the peripheral blood of pregnant woman, can prevent the cell chromosome DNA from being released, can restrain DNA enzyme degradation and is beneficial to the stability of fetus free DNA.

Description

technical field [0001] The invention relates to the field of prenatal diagnosis, in particular to a preservative for fetal free DNA in the peripheral blood of pregnant women and a vacuum blood collection tube composed of the same. Background technique [0002] Non-invasive prenatal diagnosis is mainly based on the free fetal DNA in the plasma of pregnant women. Pregnant women can detect fetal free DNA from the 7th week of pregnancy. The use of fetal free DNA can be used for early and specific prenatal screening and diagnosis. [0003] However, the amount of fetal free DNA in the plasma of pregnant women is very small, accounting for only about 5% to 7% of the total free DNA, and it is extremely vulnerable to chromosomal DNA pollution released by the rupture of maternal nucleated cells and high concentration of intracellular DNase degradation . [0004] Studies have shown that pre-analytical factors for cell-free fetal DNA testing, such as improper blood sample collection, s...

Claims

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Application Information

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IPC IPC(8): C12N5/078C12M1/24
CPCC12M23/08C12N5/0634C12N2500/12C12N2500/34C12N2500/44C12N2501/999C12M1/24
Inventor 韦德
Owner CHENGDU RICH SCI IND
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