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Ultralow molecular weight hyaluronic acid and preparation method thereof

A technology of hyaluronic acid and hyaluronidase, which is applied in the direction of fermentation, etc., can solve the problems of high waste liquid treatment cost, unreported product composition, harsh reaction conditions, etc., and achieve stable product quality, narrow molecular weight distribution range, and short production cycle short effect

Inactive Publication Date: 2020-04-21
NANJING HANXIN PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It reports the permeability of the product, and the prepared low molecular weight sodium hyaluronate has a molecular weight range of 5kDa-20kDa, but does not report the composition of the product, and uses peroxide as an oxidant to degrade macromolecular hyaluronic acid in a high-concentration alcohol solution , the reaction conditions are harsh and organic solvents are used, the cost of waste liquid treatment is high and the environmental pressure is high

Method used

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  • Ultralow molecular weight hyaluronic acid and preparation method thereof
  • Ultralow molecular weight hyaluronic acid and preparation method thereof
  • Ultralow molecular weight hyaluronic acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 enzymolysis reaction

[0050] In a 5L glass beaker, add 3L of purified water, control the stirring speed to 400rpm, control the temperature to 40°C, add hyaluronidase 1.5×10 8 U, the enzyme activity of the system is 5×10 4 U / mL, add 330g of macromolecular hyaluronic acid, after it is completely dissolved, adjust the pH of the solution to 5.5, keep the system at 40°C and stir for 24h.

Embodiment 2

[0051] Embodiment 2 enzymolysis reaction

[0052] In a 5L glass beaker, add 3L of purified water, control the stirring speed at 100rpm, control the temperature at 35°C, add hyaluronidase 1.2×10 8 U, the enzyme activity of the system is 4×10 4 U / mL, add 330g of macromolecular hyaluronic acid, after it is completely dissolved, adjust the pH of the solution to 6.0, keep the system at 35°C and stir for 24h.

Embodiment 3

[0053] Embodiment 3 enzymolysis reaction

[0054] In a 5L glass beaker, add 3L of purified water, control the stirring speed at 700rpm, control the temperature at 45°C, add hyaluronidase 3×10 8 U, the enzyme activity of the system is 1×10 5 U / mL, add 150g of macromolecular hyaluronic acid, after it is completely dissolved, adjust the pH of the solution to 5.0, keep the system at 45°C and stir for 36h.

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Abstract

The invention discloses an ultralow molecular weight hyaluronic acid and a preparation method thereof, and belongs to the technical field of biochemical engineering. Macromolecular hyaluronic acid used as a raw material undergoes production processes of hyaluronidase hydrolysis, heating inactivation, activated carbon filtration, spray drying and the like to obtain the ultralow molecular weight hyaluronic acid product with the average molecular weight lower than 1200 Daltons. The ultralow molecular weight hyaluronic acid is a mixture of hyaluronic acid disaccharide to dodecaose, the content ofthe hyaluronic acid disaccharide is 5-40 %, the content of the hyaluronic acid tetrasaccharide is 40-70 %, the content of the hyaluronic acid hexasaccharide is 10 to 30 %, the content of the hyaluronic acid octasaccharide is 1-15%, the content of the hyaluronic acid decaose is 1-10%, and the content of hyaluronic acid dodecaose or above is lower than 6%. Compared with common commercially availablelow-molecular hyaluronic acid, the product of the invention has remarkable permeation moisturizing and repair promoting capacity and can be widely applied to the fields of medical products, health care products, cosmetics and the like. The method is simple and convenient to operate, mild in conditions, free of organic solvents, high in enzymolysis efficiency and suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to an ultra-low molecular weight hyaluronic acid and a preparation method thereof. Background technique [0002] Hyaluronic acid (Hyaluronic acid, HA, macromolecular hyaluronic acid, also known as hyaluronic acid) is a class of (1-3)-2-N-acetylamino-2-deoxy-D-glucose-(1-4 )-O-β-D-glucuronic acid disaccharide repeated arrangement of an acidic straight-chain polymucopolysaccharide formed. In 1934, it was first extracted from the vitreous body of the bull’s eye by Meyer et al. It has strong hydrophilicity and very good moisturizing properties. It is the substance with the best moisturizing properties found in nature and is recognized as the most ideal by the international cosmetics industry. At the same time, because HA has no immunogenicity and toxicity, it is widely used in industries such as cosmetics, food and medicine. [0003] According to literature re...

Claims

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Application Information

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IPC IPC(8): C08B37/08C12P19/26C12P19/14C12P19/12
CPCC08B37/0072C08B37/0003C12P19/26C12P19/14C12P19/12A61P17/02A61K8/735A61K2800/10A61Q19/00
Inventor 王印成锦崔怀言徐勇刚汤传根陈松张昊宁
Owner NANJING HANXIN PHARMA TECH CO LTD
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