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Preparation method of galacto-mannan-oligosaccharides and application of galacto-mannan-oligosaccharides

A technology of galactomannan oligosaccharides and galactomannans, which is applied in the field of directional preparation of galactomannan oligosaccharides with a specific molecular weight, can solve problems such as patent vacancies, and achieve simple equipment, wide application range, and oligosaccharides high rate effect

Active Publication Date: 2019-05-28
NANJING YIXIAN BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on galactomannan oligosaccharides that promote the proliferation of butyrate-producing probiotics, and there are no patents. Therefore, the development of an oligosaccharide product that can promote the proliferation of intestinal butyrate-producing bacteria has broad application prospects

Method used

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  • Preparation method of galacto-mannan-oligosaccharides and application of galacto-mannan-oligosaccharides
  • Preparation method of galacto-mannan-oligosaccharides and application of galacto-mannan-oligosaccharides
  • Preparation method of galacto-mannan-oligosaccharides and application of galacto-mannan-oligosaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In 250mL of distilled water with pH 5.0, dissolve acid mannanase at a ratio of 2000U / g to galactomannan, then add 25g of locust bean gum, guar gum, kale gum, fenugreek gum, and Pull the glue and hydrolyze it at 45°C for 8 hours. After the enzymatic hydrolysis reaction, the reaction solution was inactivated in a boiling water bath for 10 minutes to obtain an enzymatic hydrolysis solution. After the obtained enzymolysis solution was centrifuged at 4000rpm for 20min, the precipitate was dried and weighed, and the supernatant was collected. The supernatant was intercepted by hollow fiber membranes with molecular weight cut-offs of 1000Da and 200Da respectively to obtain an oligosaccharide solution with a molecular weight in the range of 1000-200Da, which was freeze-dried to obtain dry oligosaccharide powder, which was weighed. The enzymatic hydrolysis precipitation yield and oligosaccharide yield are shown in Table 1. The results showed that when the acid mannanase was hy...

Embodiment 2

[0033] In 250mL of distilled water with pH 5.0, dissolve cellulase at a ratio of 2000U / g to galactomannan, then add 25g of locust bean gum, guar gum, kale gum, fenugreek gum, and tara gum , placed at 45 ° C for 8h hydrolysis. After the enzymatic hydrolysis reaction, the reaction solution was inactivated in a boiling water bath for 10 minutes to obtain an enzymatic hydrolysis solution. After the obtained enzymolysis solution was centrifuged at 4000rpm for 20min, the precipitate was dried and weighed, and the supernatant was collected. The supernatant was intercepted by hollow fiber membranes with molecular weight cut-offs of 1000Da and 200Da respectively to obtain an oligosaccharide solution with a molecular weight in the range of 1000-200Da, which was freeze-dried to obtain dry oligosaccharide powder, which was weighed. The yield of oligosaccharides is shown in Table 2. The results showed that when the cellulase was enzymatically hydrolyzed alone, the enzymatic hydrolysis wa...

Embodiment 3

[0037] In 250mL of distilled water with pH 5.0, dissolve acid mannanase and cellulase respectively according to the ratio of 1000U / g to galactomannan, then add 25g of locust bean gum, guar gum, squash gum, and gourd Ba gum and tara gum were hydrolyzed at 45°C for 8 hours. After the reaction, the reaction solution was inactivated in a boiling water bath for 10 minutes to obtain an enzymatic hydrolysis solution. After the obtained enzymolysis solution was centrifuged at 4000rpm for 20min, the precipitate was dried and weighed, and the supernatant was collected. The supernatant was intercepted by hollow fiber membranes with molecular weight cut-offs of 1000Da and 200Da respectively to obtain an oligosaccharide solution with a molecular weight in the range of 1000-200Da, which was freeze-dried to obtain dry oligosaccharide powder, which was weighed. The yield of oligosaccharides is shown in Table 3. The results showed that the combination of acid mannanase and cellulase for enzy...

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Abstract

The invention discloses a preparation method of galacto-mannan-oligosaccharides and application of the galacto-mannan-oligosaccharides. The preparation method comprises the following steps: (1) performing segmented composite enzymatic hydrolysis on galacto-mannan-oligosaccharides such as guar gum, sesbania gum, locust bean gum, fenugreek gum and Tara gum, wherein the enzyme is a complex enzyme ofacid mannase and cellulase; and (2) by segmented ultrafiltration classification, obtaining degraded galacto-mannan-oligosaccharides which are a mixture of a disaccharide, a trisaccharide, a tetrasccharide and a pentosaccharide. The preparation process is simple and easy, and is environmentally friendly. The conversion rate of the galacto-mannan-oligosaccharides is high, and the galacto-mannan-oligosaccharides are easy to separate. The prepared galacto-mannan-oligosaccharides can be used as prebiotics to be applied to food and animal feed, the proliferation of a butyric acid-production probiotic is promoted, and the intestinal environment is improved.

Description

technical field [0001] The invention relates to the technical field of oligosaccharide preparation, and more specifically relates to the preparation of galactomannan oligosaccharides with specific molecular weight. Background technique [0002] Butyric acid is the main energy source of intestinal mucosal epithelial cells, which can promote intestinal mucosal repair and functional damage repair, and can inhibit the production of inflammatory cytokines, thereby playing an anti-inflammatory effect. Butyric acid also has an anti-tumor effect, which can reduce the production of intestinal tumor necrosis factor and induce tumor cell differentiation and apoptosis. Butyric acid in animal intestines mainly comes from the fermentation of carbohydrates by intestinal flora. The level of intestinal butyrate depends on the type and quantity of butyrate-producing bacteria in the cecum and colon on the one hand, and on the other hand the quantity and type of carbohydrates that can be ferme...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04A23L33/21A23K20/163
Inventor 牟海津付晓丹邢坤
Owner NANJING YIXIAN BIOTECHNOLOGY CO LTD
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