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Method for detecting target breast milk oligosaccharide in formula food

A technology for breast milk oligosaccharides and formula food, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as increasing the difficulty of separation, and achieve the effects of increasing detection sensitivity, accurate qualitative and quantitative, and removing interfering impurities.

Active Publication Date: 2021-11-23
HEILONGJIANG FEIHE DAIRY
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  • Abstract
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  • Claims
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Problems solved by technology

However, the influence of the matrix and ionization suppression make the quantitative analysis of HMO difficult even if mass spectrometry is used. The components in the mixed HMO have mutual inhibition in the detection, resulting in different ionization efficiencies.
In addition, most HMOs will form anomers (double peaks), which increases the difficulty of separation

Method used

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  • Method for detecting target breast milk oligosaccharide in formula food
  • Method for detecting target breast milk oligosaccharide in formula food
  • Method for detecting target breast milk oligosaccharide in formula food

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Experimental program
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Embodiment 1

[0123] 1. Reagents and Materials

[0124] 1.1 Reagents

[0125] Unless otherwise specified, the following reagents used were of analytical grade, and each of the following reagents used were provided by Sinopharm.

[0126] 1.1.1 Water: GB / T 6682, Class I.

[0127] 1.1.2 Sodium borohydride (NaBH 4 ), analytically pure.

[0128] 1.1.3 Acetic acid (CH 3 COOH), analytically pure.

[0129] 1.1.4 Ammonium acetate (CH 3 COONH 4 ), analytically pure.

[0130] 1.1.5 Acetonitrile (CH 3 CN): chromatographically pure.

[0131] 1.2 Reagent preparation

[0132] 1.2.1 Sodium borohydride solution (0.25mol / L): Weigh 0.95g of sodium borohydride (1.1.2) into a 100mL beaker, add 80mL of water (1.1.1), magnetically stir to dissolve, transfer to 100mL polyethylene In the volumetric flask, adjust the volume to the mark with water (1.1.1), and prepare it immediately after use.

[0133]1.2.2 Acetic acid solution (0.25mol / L): Take 0.75mL of acetic acid (1.1.3), dilute to 50mL with water (1....

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Abstract

The invention relates to a method for detecting target breast milk oligosaccharide in formula food. The target breast milk oligosaccharide may include or be one or more of 2 '-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6 '-sialyllactose, lactose-difucosyltetraose, lactose-N-neotetraose, and lactose-N-tetraose. The method comprises the following steps: removing fat and protein from a sample, reducing by adopting a reducing agent such as NaBH4, detecting by adopting liquid chromatography-mass spectrometry, and quantifying by adopting an external standard method. According to the method, the target breast milk oligosaccharide (HMO) can be simply, conveniently, quickly, accurately, qualitatively and quantitatively detected.

Description

technical field [0001] The invention relates to the field of food. Specifically, the present invention relates to a method for detecting target breast milk oligosaccharides in formula food. Background technique [0002] Breast milk is the most natural and high-quality food for newborns. Breast milk contains various nutrients, such as protein, fat, carbohydrates, inorganic salts and trace elements. Their reasonable ratio is suitable for infants to digest and absorb. In addition, breast milk also contains biological activity Substances that have short-term anti-infective benefits. [0003] Human milk oligosaccharides (HMO) are the third largest solid component in human breast milk, second only to lactose and fat. Human milk oligosaccharides are free polysaccharides composed of 3-22 monosaccharide units. According to the presence or absence of sialic acid, HMOs can be divided into two categories: neutral HMOs and acidic HMOs. The reducing ends of HMOs all contain lactose. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 蒋士龙解庆刚王象欣刘莹崔东影梁爱梅冷友斌
Owner HEILONGJIANG FEIHE DAIRY
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