1129results about How to "Simple extraction method" patented technology

This invention discloses a method for extracting high-purity glucoside of Rubas suavissimus. The method comprises: extracting Rubas suavissimus with water or mixed solvents of water and ethanol under refluxing, adsorbing by resin, eluting, removing impurities by column chromatography, concentrating, drying to obtain powdery crude product, dissolving with absolute ethanol by heating, filtering, and crystallizing to obtain high-purity glucoside. The method is simple, and the product has such advantages as high purity and high sweetness. The glucoside can be used to produce high-sweetness healthcare products or drugs with low caloric value.

The invention discloses Agrobacterium ZX09, water-soluble beta-glucan prepared from the Agrobacterium ZX09 and the preparation method thereof and the application on reducing blood sugar, wherein the bacterial strain of the Agrobacterium ZX09 is obtained by separating saline soil from Dongying city in Shandong province; the bacterial strain is Agrobacterium sp. and is collected in China Center for Type Culture Collection (CCTCC) on January 21st, 2010; and the collection number is CCTCC NO:M2010020. The structure general formula of the water-soluble beta-glucan prepared from the Agrobacterium ZX09 is as follows: ->(3)-beta-D-Glup-(1->3)-[beta-D-Glup-(1->3)-beta-D-Glup-]3-alpha-D-Glup-(1->3)-alpha-D-Glup-(1)n->, and n in the structural formula is from 100 to 200. The bacterial strain has the advantages of novelty, special properties, salt resistant growth, no pigment secretion in the process of growth, simple and convenient cultivation operation, simple technology, total synthetic media, definite components and low cost, and is suitable for industrial production.

A seed treating agent for promoting the germination of plant seeds, especially the seeds of cistanche, contains gibberelline, naphthylacetic acid, cytokinin, carbendazim, and the optional one of the extracts of three plant roots (tamaisk root, vetchleaf sophora root, etc).

Provided is a heavy metal pollution soil repairing agent using excess activated sludge as the rawmaterial, a method of extraction and a method for repairing the heavy metal pollution soil, which relates to a heavy metal pollution soil repairing agent and a method thereof and a method for repairing the heavy metal pollution soil, and solves the problems of high cost, easily secondary pollution and complex operation existing in the repair of the heavy metal pollution soil. The heavy metal pollution soil repairing agent is composed of amylose, protein, nucleic acid, phosphoric acid, amino acid, humic acid compound, uronic acid and the organic substance required for cell lifecycle. The method for distilling the heavy metal pollution soil repairing agent is as follows: the wastewater treatment excess activated sludge reacts under the condition of 80-120 KPa and 60-100 DEG for 8-20 minutes; then centrifuges for 8-20 min at the rotating speed of 5000-7000r / min. The invention adopts the home position repairing or the heterotopia repairing to repair the heavy metal pollution. The invention has low cost and no secondary pollution, simple operation, short repairing period, strong activity and wide range of application.

The invention discloses a parameter extraction method of a silicon cell photovoltaic assembly five-parameter model. Firstly, four electrical property parameters of a four-parameter model are obtained through parameter values provided by an assembly manufacturer nameplate, then current equation differential values of circuit current and open-circuit voltage are solved according to the values obtained through the four-parameter model and by means of an approximate solving method, and finally five parameters needed by the photovoltaic assembly five-parameter model are obtained. By means of the method, the problem that a part of needed parameters can not be directly obtained from nameplate parameters provided by a manufacturer in the parameter extraction processing of the five-parameter model in the prior art, the extraction method of the five-parameter model is further simplified, and the method can be applied to engineering practice more easily.

The present invention relates to the new use of narigenin and similar compounds in preparing medicines for treating cardiac and cerebral vascular diseases. These compounds include narigenin and its derivative, hesperidene and its derivative, isosakuranetin and its derivative, eriodictyol and its derivative, etc. These compounds may be used in preventing, diagnosing and treating cerebral infarction, myocardial infarction, cerebral ischemia-reperfusion injury, myocardial ischemia-reperfusion injury and other cardiac and cerebral vascular diseases as well as Alzheimer disease and its relevant diseases. These compounds have high pharmacological action, low toxicity, wide material source, low cost and other advantages.

The invention relates to a method for extracting a natural Chinese medicinal herb fragrance component and application of an extract thereof. The extracting method comprises the following steps: using natural Chinese medicinal herbs such as indigowoad root, fig, couch grass root and the like as main raw materials; and extracting a final black brown liquid with light sweet fragrance through the steps of mixing, primary extracting, condensing and reheating, wherein the extract is dissolved by 60 percent ethanol and then sprayed to tobacco shreds by adopting an atomizing mode to be coiled into cigarette, and the product has obvious fragrance in the cigarette. According to different physicochemical properties of substances to be extracted, the substances are extracted by using mixed solvents with different concentrations through a microwave method; therefore, the extracting and heating time is shortened, the energy is saved, the extraction efficiency and the yield are improved, and the effective components in the raw materials are obtained; a plurality of new fragrant substances such as pyrazine, pyrrole, furan and the like are produced during the reaction, so the fragrance increasing effect is more remarkable; and the extracting method is simple, the equipment is easy to operate, and the method has no pollution to the environment.

The invention discloses a method and a system for denitrifying low-carbon high-nitrogen sewage by utilizing a plant carbon source-enhanced artificial wetland. The method comprises the following steps: hydrolyzing by taking waste crops or wetland plant straws as carbon sources, and charging hydrolysate into an artificial wetland so as to improve the denitrification efficiency of the low-carbon high-nitrogen sewage. The invention simultaneously provides a treatment system, which is simple in structure, and scientific and reasonable in carbon source charging mode, and the denitrification effect of the low-carbon high-nitrogen sewage can be well realized by enhancing the artificial wetland through utilizing the plant carbon source. According to the method, the adopted plant carbon sources are cheap and easily available, the carbon release capability is strong, the bio-denitrification effect is good, and the hydrolysate is simple and practical to prepare, the carbon source charging mode is strong in continuous running capability, and the method is simple to maintain, less in secondary pollution, low in production cost, and capable of solving the technical difficulty of treating tail water in a sewage treatment plant with low carbon nitrogen ratio through artificial wetland by utilizing a simple method.

The invention discloses a method for extracting pectin from orange peel and shaddock peel, which relates to the technical field of biochemical pharmacy. A technical scheme provided in the invention is as follows: the method for extracting pectin from orange peel and shaddock peel comprises the successive steps of cleaning leftovers of orange peel and shaddock peel, crushing the leftovers of orange peel and shaddock peel into particles with a particle size of 2 to 3 mm, treating the obtained particles with steam or boiling water for 5 to 8 min to passivate the activity of pectase, steeping the particles in clear water for 30 min, heating an obtained mixture to a temperature of 90 DEG C for 5 min, carrying out rinsing with clear water several times to remove bitter, pigments and soluble impurities, and after the above-mentioned pre-treatment, carrying out mixing and homogenizing, press filtration, ultrafiltration, nanofiltration I, nanofiltration II, stirring for pectin formation, press filtration, distillation and recycling, spray drying and crushing so as to prepare a pure product. Compared with the prior art, the invention has the following striking substantive advantages and remarkable progress: usage of traditional extraction methods and harmful organic solvents is reduced, original biological activity is retained during the process of extraction, the extraction process is simple and environment-friendly, and the method costs little and is applicable to large scale industrial production.

The invention discloses a Lamb wave damage probability imaging method for damage monitoring of a composite plate structure, which belongs to the field of monitoring of engineering structure health of composite plates. According to the invention, the technology of excitation / sensing arrays is utilized; reference signals of the composite plate structure in a sound state and monitoring signals of the composite plate structure in a damage state are acquired by loading Lamb wave; then the means of wavelet analysis is employed to extract an energy difference coefficient of damage, and the position where damage is located is predicated; finally, a structure damage image is obtained by using the probability imaging method. The invention enables rapid and effective damage identification of the composite plate structure to be realized and high precision in damage identification to be obtained, and has a good engineering application value.

The invention relates to a hyperspectral image spectral-spatial cooperative classification method based on an SAE depth network. According to the hyperspectral image spectral-spatial cooperative classification method based on the SAE depth network, the conventional method that neighborhood information is applied to act as spatial features after PCA dimension reduction, spatial position information, i.e. row and column coordinates, of current pixel points are applied to act as the spatial features and then the spatial features are combined with spectral features to act as the spectral-spatial cooperative features of training samples can be substituted. The beneficial effects of the hyperspectral image spectral-spatial cooperative classification method based on the SAE depth network are that the conventional method of spatial feature extraction in spectral-spatial cooperative classification based on the SAE depth network is improved, the spatial position information is applied to act as the spatial features rather than the conventional method of spatial feature extraction that principal component analysis (PCA) dimension reduction is performed in spectral space and then neighborhood space information is extracted to act as the spatial features, the extraction method of the spatial features is simplified, computational burden is reduced and classification accuracy is enhanced in comparison with that of the conventional method.

The invention relates to a method for preparing phosphatidylserine and phosphatidylcholine, and belongs to the field of food health-care products. The method comprises the following steps of: extracting soybean meal phospholipid which is used as a raw material by an organic solvent, and then distilling and drying to respectively obtain a phosphatidylcholine crude product and a phosphatidylserine crude product; and separating and purifying the phosphatidylcholine crude product and the phosphatidylserine crude product through a silica gel column of 200-300 meshes, eluting with a mixed organic solvent, concentrating the eluant, and drying to obtain phosphatidylserine and phosphatidylcholine. According to the method, the purities of the phosphatidylserine and the phosphatidylcholine meet requirements of new resource foods, so that the phosphatidylserine and the phosphatidylcholine can be used as new resource dietary supplements and have high economic added values; and the phosphatidylserine and the phosphatidylcholine are prepared at the same time by effectively combining simple extraction of the phosphatidylserine and the simple extraction of the phosphatidylcholine. By the method, the production time is shortened, the production cost is reduced, the production rate is improved, the operation is simple, and the products have safety.

The invention relates to a method for extracting auricularia auricula polysaccharides, which comprises the following processes: soaking; adding complex enzyme and prolease, and extracting; taking supernate through centrifuging; obtaining a crude product through ethanol precipiration; refining the crude product, and the like. The extracting method is simple and convenient; and the extracted auricularia auricula polysaccharides contain three polysaccharides, namely crude polysaccharide (FA), acid polysaccharide (FA-A) and neutral polysaccharide (FA-N). The extracted auricularia auricula polysaccharides are prepared into proper medicaments for resisting ischemic cardiovascular and cerebrovascular diseases clinically, are favorable for further developing medicaments and foods related to the auricularia auricula polysaccharides, improve processing added value of black fungus and greatly improve the economic benefit of the black fungus.

The invention discloses a continuous extraction method for pectin, tannin and pigment in a persimmon pericarp, which comprises the following steps: a. after the persimmon pericarp is pretreated, the pretreated persimmon pericarp is extracted by water at a temperature which is more than or equal to 70 DEG C and is less than 100 DEG C to obtain an extract and pulp stock; b. the extract is filtered by an ultrafiltration membrane with a cutoff molecular weight of between 10000 and 30000, the trapped part is a pectin liquid, a filtrate is absorbed and separated by a macroporous resin to obtain the tannin; and c. the pulp stock obtained in step a is extracted by acetone at a temperature of between 60 and 70 DEG C, and an extraction liquid is filtered, distilled and dried to obtain the pigment. The method has rich sources of raw materials, is simple, and is more suitable for large-scale industrial production; a used reagent has lower price, small toxicity, and strong maneuverability, and is easy to amplify the technology, and the cost is lower than extraction cost of corresponding products at present. A product extracted by the method is safe and nontoxic, and has simple and reasonable production technology.

The invention discloses a method for extracting alga-inhibiting active ingredients from Artemisia annua L., and an alga-inhibiting method, and belongs to the field of water pollution control. The invention solves the problems of high cost, large ecological risk, secondary pollution and the like of the traditional alga-inhibiting technology. The device discloses a method for extracting and preparing an alga-inhibiting agent from land plant of Artemisia annua L. The method comprises the following steps of: extracting the dried and crushed Artemisia annua L. by steps with petroleum ether and ethyl acetate, washing petroleum ether extracting solution with diluted acid, combining active ingredients obtained by diluted alkaline stripping and active ingredients obtained by washing ethyl acetate extracting solution with diluted acid and diluted alkaline, and then preparing the alga-inhibiting agent. The method has the advantages of simple process, easy implementation, low cost and the like, and can be widely used for waters of ponds, reservoirs, lakes and the like.

The invention discloses a method for extracting a cesium salt from brine. The cesium salt extraction method comprises the following steps: mixing the brine with an adsorbent, taking out the adsorbent after complete adsorption, and desorbing the adsorbent by adopting a desorption agent to obtain a first pregnant solution; carrying out evaporative concentration on the first pregnant solution, carrying out cooling crystallization, and separating to obtain a first crystal solid and a second pregnant solution; and adjusting the alkalinity of the second pregnant solution, carrying out fractioning extraction, separating to obtain a loaded organic phase and a raffinate, stripping the loaded organic phase by using a stripping agent to obtain a stripping solution which is a cesium salt solution. The invention also discloses a method for extracting a rubidium salt. Compared with traditional methods for extracting the rubidium salt and the cesium salt from the brine, the methods disclosed in the invention adopting adsorption enrichment, fractioning extraction and stripping to obtain the cesium salt solution and a rubidium salt solution have the advantages of simple process, effective extraction and separation of the rubidium salt and the cesium salt, and prevention of the loss of the rubidium salt and the cesium salt.

The invention relates to spirulina phatensis polysaccharide and an extraction method thereof. The extraction method comprises the following steps of: immersing spirulina which is taken as a raw material at room temperature for seven days by using industrial ethanol, pouring ethanol, airing solid, carrying out extraction for many times by using boiling water, dialyzing fluid water for two days, after concentrating intra-dialysate, centrifuging, adding a trichloroacetic acid solution to eliminate protein, neutralizing by adding an NAOH solution till the pH value is 6.5-7.5, after dialyzing and concentrating fluid water, centrifuging, adding triploid product of industrial ethanol into supernate, standing for one night, centrifugally collecting precipitation, and drying after washing through absolute ethyl alcohol and acetone respectively so as to obtain crude aqueous extraction spirulina phatensis polysaccharide; and carrying out solid residue alkali extraction to obtain crude alkali extraction spirulina phatensis polysaccharide, and separating and eluting crude polysaccharide so as to obtain pure polysaccharide. The chemical structure of the spirulina phatensis polysaccharide is characterized in that a main chain is composed of 1,4-connection alpha-D-glucose; average every eight main chain residues have one branch; substitution is at the sixth position of a branch point; and the branch is composed of one or two 1.6 connection glucose. The extraction method of the spirulina phatensis polysaccharide disclosed by the invention is simple and convenient and has good reproducibility.

The invention discloses a gas chromatography for detection of a phosphate fire-retardant plasticizer in a textile. The gas chromatography comprises the following steps of 1, preparing a test sample solution which is supernatant, 2, preparing a gradient standard solution having the concentration of 1 to 10 micrograms per milliliter by dissolving at least one phosphate fire-retardant plasticizer in a solvent, 3, injecting the gradient standard solution into a gas chromatograph, carrying out detection by a flame photometric detector to determinate peak positions of the at least one phosphate fire-retardant plasticizer, recording corresponding peak areas, and making a standard curvilinear equation by concentrations as horizontal coordinates and the peak areas as vertical coordinates, and 4, replacing the gradient standard solution used in the step 3 with the supernatant obtained by the step 1, detecting the at least one phosphate fire-retardant plasticizer in the test sample solution and the peak areas of the at least one phosphate fire-retardant plasticizer according to the step 3, and carrying out calculation by the standard curvilinear equation obtained by the step 3 to obtain the content of the at least one phosphate fire-retardant plasticizer in the test sample solution.

The invention discloses a method for extracting krill oil rich in phospholipid from euphausia superba. The method comprises the following steps of: A, drying; B, extracting; C, volatilizing a solvent; and D, concentrating phospholipid. Through the four steps, the phospholipid content of the obtained krill oil is high and more than 50 percent, the quality is good, and the obtained krill oil also contains multiple elements which are beneficial to the human body such as astaxanthin, unsaturated fatty acid, vitamin and trace elements; the concentrating step of the phospholipid adopts polarity organic solvent as a solvent, so that the residual extracting solvent in the krill oil can be further removed from the euphausia superba; not only can the euphausia superba krill oil with high phospholipid content be obtained, the euphausia superba krill oil on the upper solution in the concentration step also can be recycled, and the extracted euphausia superba powder also can be used for producing other products; and the extracting method is simple, the obtained product is stable in quality, and the used solvent is relatively safe and practical.

The invention relates to an antibacterial tanshinone extract extracted from Salvia spp. roots, a preparation method thereof, application of the antibacterial tanshinone extract and products of the antibacterial tanshinone extract. The extract contains cryptotanshinone, dihydro-tanshinone, tanshinone I and tanshinone IIA, wherein the total weight content of the four compounds is 40 to 99 percent of the total weight content of the extract; the weight content of the cryptotanshinone is 50 to 95 percent of the total weight of the four compounds; the weight content of the dihydro-tanshinone is 5 to 30 percent of the total weight of the four compounds; the weight content of the tanshinone I is 0 to 20 percent of the total weight of the four compounds; and the weight content of the tanshinone IIA is 0 to 40 percent of the total weight of the four compounds. The preparation method comprises the steps of diacolation extraction and purification for two times. The extract is used for preparing antibacterial medicines or other products. The tanshinone extract obtained has high purity, more reasonable proportions of various compositions, and can exert better clinic effect.

The invention discloses a bacterial strain for producing PMLA [Poly (Beta-L-malic acid)], which is obtained by screening the surface of a plant leaf and can not produce melanin. The classification name is Aureobasidium pullulans, the Latin literature name is Aureobasisdium pullulans ZD-3D, and the preservation number of the bacterial strain is CGMCC No.4605. The method for producing PMLA by the bacterial strain is simple to implement and is beneficial to the separation and purification of PMLA, and the yield can be up to 50-60g / L.

The invention provides a human body anti-AQP4 autoantibodies detection material and a preparation method thereof. The preparation method comprises the steps of firstly acquiring M1 and M23 subtype AQP4 overall length target genes, dyeing into an HEK293 cell, and building an HEK293 / pCI-neo-M1-AQP4 cell and an HEK293 / pCI-neo-M23-AQP4 cell expressing M1 and M23 subtype AQP4; extracting an AQP4-cell membrane complex of the cells; finally curing the AQP4-cell membrane complex on a carrier, and obtaining the human body anti-AQP4 autoantibodies detection material. According to the human body anti-AQP4 autoantibodies detection material and the preparation method thereof provided by the invention, the extracted AQP4-cell membrane complex is used as a detection material, so that the intrinsic protein amount causing background coloration can be reduced; compared with a method for using the cell as the detection material for carrying out immunofluorescence detection, the detection sensitivity and the specificity are further increased, a detection result is easier and clear to judge, and the AQP4 autoantibodies of a patient can be simply, conveniently and quickly detected.

The invention discloses 3-phenylcoumarin robustic acid as well as an extraction method and application thereof. 3-phenylcoumarin robustic acid has a structural formula as shown in the specification as well as a chemical name of 3-(p-methoxyphenyl)-4-hydroxy-5-methoxy-2', 2'-dimethyl-6,7-dihydropyrancoumarin, a molecular formula of C22H20O6, and relative molecular weight of 380. 3-phenylcoumarin robustic acid disclosed by the invention has anti-tumor and other biological activities, can be effectively applied in preparation of anti-tumor drugs and has very strong inhibition effect on human gastric cancer cells, liver cancer cell lines, human lung cancer cells, cervical cancer cells, ovarian cancer cells and the like, the extraction method is simple and feasible and the extraction efficiency is relatively high.

The invention discloses a method for detecting a pear endogenous hormone by using an ultra-high performance liquid chromatography electrospray tandem mass spectrum. The method comprises the following steps of: crushing obtained pear tissues, then adding an extracting solution for extraction, after ending extraction, adding an extraction reagent to a system, and extracting to obtain a crude extract of the endogenous hormone; separating and purifying the crude extract by using a solid-phase extraction column to obtain a detection sample; analyzing a detection sample by using the ultra-high performance liquid chromatography electrospray tandem mass spectrum to obtain the variety and the content of the endogenous hormone in the pear tissues, wherein the extracting solution comprises isopropanol, water and hydrochloric acid, and the volume ratio of the isopropanol, the water and the hydrochloric acid is (1.5-2.5):1:(0.001-0.002), and the extraction agent is dichloromethane. The method has the advantages of high sensitivity, simple pretreatment, high recovery rate, high degree of accuracy and degree of precision and the like and is used for detecting and confirming the endogenous hormones in multiple plant issues.

The invention discloses a method for extracting tripterygium glycosides, and a product and an inclusion compound and a medicinal composition thereof. The method for extracting the tripterygium glycosides comprises the following steps of: loading a uniform mixture obtained by crushing common threewingnut root and an adsorbent to a silicagel column, adding chloroform for impregnating, eluting by using the chloroform until eluent is colorless, eluting by using mixed liquor of ethanol and the chloroform in a mass ratio of 4.5:95.5-10:90, and collecting ethanol and chloroform eluent. In the extraction method, the steps are simple, the using amount of an organic solvent is small, waste water is hardly generated, the organic solvent can be basically recycled, pollution is reduced, energy consumption is greatly reduced, and production cost is reduced; and the prepared tripterygium glycosides have high active ingredient content, particularly high wilforlide A content, and meet the national standard; a medicinal preparation can be directly prepared without the separation and purification of the tripterygium glycosides; and tripterygium glycoside tablets have high dissolution rate and bioavailability.

The invention provides a pig feed additive capable of effectively improving pig immunity and promoting pig growth. The pig feed additive is composed of astragalus polysaccharide, acanthopanax powder, fermented traditional Chinese medicine powder, muskmelon pedicel powder, tangerine peel powder and the rest carrier, the carrier is a mixture of wheat bran and defatted rice bran at a mass ratio of 1:1. The pig feed additive can remarkably lower incidence of pigs, improve immunity of the pigs and increase growth rate of the pigs, can replace or partly replace application of chemosynthesis additives in feed and has wide market prospect.

The present invention discloses the extraction process of lotus leaf flavone. Fine lotus leaf flavone powder is prepared with lotus leaf as material and through five links of pre-treatment, percolating extraction, macroporous resin adsorption to separate, purification with organic solvent and post-treatment. The fine lotus leaf flavone powder of the present invention has stable chemical property, less impurity, high water solubility and other features; has flavone content of 50-70 wt%; and reaches the requirement as class-II medicine material.

The invention relates to an isoflavone compound having antibacterial activity and a preparing method and applications thereof, and belongs to the technical field of phytochemistry. The structure of the compound is shown as a formula (I). The formula (I) is shown in the specification. The compound is prepared from kudzu vine root that is a traditional medicinal and edible plant and through steps of extractum extraction, column chromatography on silica gel and separation and purification through high performance liquid chromatography. Activity tests prove that the compound has a good bacteriostatic function; when the compound is added into a tobacco material solution, the compound can effectively inhibit microbe growth in the material solution and can prolong the shelf life of the solution; when the compound is used for cigarette tipping paper, the compound can eliminate or reduce the possibility of bacterium breeding and reproduction in the cigarette tipping paper; and the compound has a wide application prospect.