The invention aims at providing an euphausia superba peptide replenisher containing calcium, iron and zinc. The euphausia superba peptide replenisher containing calcium, iron and zinc is obtained by combining calcium, iron and zinc metal elements with euphausiid peptide powder prepared by euphausia superba powder defatting, protease degradation and CaCl2 defluorination. The adopted raw materials of the euphausia superba peptide replenisher containing calcium, iron and zinc provided by the invention are abundant, easily available, and high in protein content; the preparation method has the advantages of being simple in process steps, low in equipment investment, free from environmental pollution, high in product yield and the like, and is suitable for industrialized production; the prepared euphausiid peptide-calcium, iron and zinc replenisher has the characteristics that the protein content is higher than 90%, and thus the product purity is high; the fluorine content is lower than 10 ppm, and thus the product safety is high; the molecular weight is mainly distributed between 2000-200 Da, and thus the water solubility is good; the essential amino-acid content is high, the composition of calcium, iron and zinc is reasonable, and three nutrients and essential amino-acids can be replenished simultaneously.