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Method of preparing proteins, chitin and chitosan from degreased shrimp meals of euphausia superba

A technology of Antarctic krill and protein, which is applied in the field of comprehensive utilization of Antarctic krill, can solve the problems of large resource consumption, environmental pollution by waste liquid and waste residue, low utilization rate of raw materials, etc., achieve simple process technology, reduce environmental pollution, Consumption saving effect

Inactive Publication Date: 2014-03-05
青岛福创环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the Antarctic krill in China mainly only extracts krill oil, astaxanthin or chitin and other products. During the production process, a large amount of waste liquid and waste residue pollute the environment, the utilization rate of raw materials is low, and the resource consumption per unit product is large. Efficient use of Antarctic krill resources, through a production process, to achieve high-efficiency output of multiple products, greatly reduce product production costs, greatly increase the additional output value of products, and realize high-value and efficient comprehensive utilization of krill resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for preparing protein, chitin, and chitosan from Antarctic krill defatted shrimp powder, the steps comprising:

[0015] 1. Protein extraction: add 1% NaOH solution to defatted krill powder, the solid-to-liquid ratio is 1:10, heat in a water bath at 70°C for 1 hour, filter and separate the filtrate, wash the filter residue fully with water, combine the filtrate and washing liquid, Use hydrochloric acid to adjust to the isoelectric point of the protein, pH=4.5, precipitate the protein, centrifuge, collect the precipitate, and obtain the protein after spray drying;

[0016] 2. Preparation of chitin: Use 5% NaOH solution to filter the protein-extracted residue, with a solid-to-liquid ratio of 1:5, keep it in a water bath at 80°C for 1 hour, filter, and wash fully with water to obtain deproteinized shrimp shells; the deproteinized Shrimp shells were soaked in EDTA solution for more than 4 h, filtered, washed with an appropriate amount of weak alkaline water, and the...

Embodiment 2

[0020] Add 3% NaOH solution to defatted krill powder, the solid-to-liquid ratio is 1:12, heat in a water bath at 80°C for 2 h, filter and separate the filtrate, wash the filter residue with water, combine the filtrate and washing liquid, and adjust to Protein isoelectric point, pH=5.5, precipitate protein, centrifuge, collect precipitate, and get protein after spray drying;

[0021] Use 8% NaOH solution on the filter residue after protein extraction, with a solid-to-liquid ratio of 1:10, keep it warm in a water bath at 90°C for 2 hours, filter, and wash fully with water to obtain deproteinized shrimp shells; use EDTA solution for the deproteinized shrimp shells Soak for more than 4 hours, filter, wash with an appropriate amount of weak alkaline water, collect the filtrate and washing liquid; deproteinize the filter residue with 5% NaOH solution once, filter, and combine the filtrate with the previous mixed solution for EDTA recovery; Protein and decalcified filter matter were ...

Embodiment 3

[0024] Add 2% NaOH solution to defatted krill powder, the solid-to-liquid ratio is 1:11, heat in a water bath at 75°C for 1.5 hours, filter and separate the filtrate, wash the filter residue with water, combine the filtrate and washing liquid, and adjust to Protein isoelectric point, pH=5.0, precipitate protein, centrifuge, collect precipitate, and get protein after spray drying;

[0025] Use 6% NaOH solution on the filter residue after protein extraction, with a solid-to-liquid ratio of 1:7, keep it in a water bath at 85°C for 1.5 hours, filter, and wash fully with water to obtain deproteinized shrimp shells; use EDTA solution for the deproteinized shrimp shells Soak for more than 4 hours, filter, wash with an appropriate amount of weak alkaline water, collect the filtrate and washing liquid; deproteinize the filter residue with 4% NaOH solution once, filter, and combine the filtrate with the previous mixed solution for EDTA recovery; Protein and decalcified filter matter wer...

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PUM

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Abstract

The invention discloses a method of preparing proteins, chitin and chitosan from degreased shrimp meals of euphausia superba. The method comprises the following steps: 1, extracting the proteins; 2, preparing the chitin; 3, preparing the chitosan. By adopting the method, the proteins with high purity, the chitin and the chitosan with high deacetylation degree are prepared from waste shrimp meals used as raw materials, and effective development and utilization of euphausia superba resources are realized; as EDTA (Ethylene Diamine Tetraacetic Acid) is adopted to carry out decalcification, the decalcification effect is good, the decalcification rate of once decalcification can reach above 95 percent, the EDTA also has a decoloration effect during the decalcification, and the dosage of hydrogen peroxide used as a subsequent decolorant is greatly saved; the used EDTA can be recycled and reused, the recovery rate can reach above 85 percent; in addition, as the reaction is carried out under the neutral and slight alkaline conditions, the characters of the obtained chitin are more stable than those of the chitin obtained by virtue of a hydrochloric acid method, and the product performance is greatly improved; the whole process technology is simple, waste water, waste slag and organic reagents generated in the production process can be all recycled and turned into wealth, and the environment pollution is also lowered.

Description

technical field [0001] The invention relates to a method for preparing protein, chitin and chitosan from defatted shrimp powder of Antarctic krill, and belongs to the technical field of comprehensive utilization of Antarctic krill. Background technique [0002] Antarctic krill contains high-quality lipids, excellent protein, rich amino acids, natural colorants and antioxidant astaxanthin and other active ingredients; these ingredients make Antarctic krill an irreplaceable aquatic animal resource, and its use in aquatic products The application in is more and more extensive, and exerts incomparable advantages. At present, the development and application forms of Antarctic krill products mainly include: 1) Production of nutritional and health food with high added value - Antarctic krill oil; 2) Production of high-efficiency aquaculture animal feed - Antarctic krill meal; 3) Production of Antarctic krill Protein products - krill protein peptide, protein powder, etc.; 4) Produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C08B37/08
Inventor 任宪君孙来娣张秋艳
Owner 青岛福创环境科技有限公司
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