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Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain

A technology of polymalic acid and bacterial strains, which is applied in the field of polymalic acid-producing bacterial strains and its fermentation to produce polymalic acid, which can solve the problems of high cost and low efficiency, and achieve simple raw materials, simple extraction methods, and simple and easy methods Effect

Inactive Publication Date: 2011-10-19
SHANDONG JINCHENG BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, biological synthesis has great advantages, but its development and application are restricted by high cost and low efficiency. At present, there is no optimized route for industrial production, and only a small amount of natural polymalic acid and artificially synthesized polymalic acid can be produced. supply

Method used

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  • Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]1) Take a loop of Aureobasidium pullulans ZD-3D slant strain cultured at 25°C for 48 hours, and inoculate it into a liquid seed medium. The liquid volume is 60ml of medium in a 500ml Erlenmeyer flask, the rotation speed is 220rpm, and cultivated at 25°C for 48h to obtain seeds liquid;

[0035] 2) Inoculate the prepared seed solution into the fermentation medium at a ratio of 10%, 60ml of the medium in a 500ml Erlenmeyer shaker flask, at a rotation speed of 220rpm, at 25°C, and incubate for 160h;

[0036] The above seed medium is: 80g / l glucose, 2g / l sodium nitrate, 0.04% K 2 CO 3 ,0.01%KH 2 PO 4 , 0.01%MgSO 4 ·7H 2 O, 0.01% ZnSO 4 ·7H 2 O, 0.5g / l corn steep liquor, 20g / l calcium carbonate;

[0037] The above fermentation medium: 120g / l glucose, 2g / l sodium nitrate, 0.04% K 2 CO 3 ,0.01%KH 2 PO 4 , 0.01%MgSO 4 ·7H 2 O, 0.01% ZnSO 4 ·7H 2 O, 0.5g / l corn steep liquor, 30g / l calcium carbonate.

[0038] Calcium carbonate is sterilized alone and mixed with oth...

Embodiment 2

[0043] 1) Take a loop of Aureobasidium pullulans ZD-3D slant strain cultured at 25°C for 48 hours, and inoculate it into a liquid seed medium. The liquid volume is 60ml of medium in a 500ml Erlenmeyer flask, the rotation speed is 220rpm, and cultivated at 25°C for 48h to obtain seeds liquid;

[0044] 2) Inoculate the prepared seed solution into the fermentation medium at a ratio of 10%, 60ml of the medium in a 500ml Erlenmeyer shaker flask, at a rotation speed of 220rpm, at 25°C, and incubate for 160h;

[0045] The above seed medium is: 80g / l glucose, 2g / l sodium nitrate, 0.04% K 2 CO 3 ,0.01%KH 2 PO 4 , 0.01%MgSO 4 ·7H 2 O, 0.01% ZnSO 4 ·7H 2 O, 0.5g / l corn steep liquor, 20g / l calcium carbonate.

[0046] The above fermentation medium: 120g / l sucrose, 2g / l sodium nitrate, 0.04% K 2 CO 3 ,0.01%KH 2 PO 4 , 0.01%MgSO 4 ·7H 2 O, 0.01% ZnSO 4 ·7H 2 O, 0.5g / l corn steep liquor, 30g / l calcium carbonate.

[0047] Calcium carbonate is sterilized alone and mixed with ot...

Embodiment 3

[0050] 1) Same as embodiment 1.

[0051] 2) Same as Example 1, the difference is: fermentation medium: 120g / l glucose, 2g / l NH 4 NO 3 , 0.04%K 2 CO 3 ,0.01%KH 2 PO 4 , 0.01%MgSO 4 ·7H 2 O, 0.01% ZnSO 4 ·7H 2 O, 0.5g / l corn steep liquor, 30g / l calcium carbonate.

[0052] 3) Same as embodiment 1.

[0053] As measured by the PMLA yield, the yield can reach 50.75g / l.

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Abstract

The invention discloses a bacterial strain for producing PMLA [Poly (Beta-L-malic acid)], which is obtained by screening the surface of a plant leaf and can not produce melanin. The classification name is Aureobasidium pullulans, the Latin literature name is Aureobasisdium pullulans ZD-3D, and the preservation number of the bacterial strain is CGMCC No.4605. The method for producing PMLA by the bacterial strain is simple to implement and is beneficial to the separation and purification of PMLA, and the yield can be up to 50-60g / L.

Description

technical field [0001] The invention relates to a high-yielding polymalic acid [poly (β-L-malic acid), PMLA] strain and a method for fermenting and producing polymalic acid using the strain. Background technique [0002] Polymalic acid [poly (β-L-malic acid), PMLA] is a special kind of aliphatic polyester formed by malic acid as the only monomer and linked by ester bonds. It is a new natural polymer. A biopolymer developed. PMLA is made of malic acid as the basic structural unit, and the -OH of one malic acid molecule is connected with the α or β-COOH of another malic acid molecule. At present, three kinds of PMLA structures have been found: α-type, β-type and γ-type polymalic acid can be synthesized by chemical or biological methods, and both methods are currently being studied. Three types of polymalic acid can be obtained through chemical synthesis; and the polymalic acid isolated from microbial cells has a β type. [0003] Polymalic acid contains many free carboxyl gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/62C12R1/645
Inventor 徐志南张慧莉黄磊蔡谨
Owner SHANDONG JINCHENG BIO PHARMA CO LTD
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