192 results about "Acid molecule" patented technology

Methods and compositions suitable for accomplishing the in vitro amplification of nucleic acid molecules via enzymatic means are provided. The preferred means employ circular rather than linear replicons. Means for producing such circular replicons from linear reactants are also provided.

This invention generally relates to nucleic acid synthesis, in particular DNA synthesis. More particularly, the invention relates to the production of long nucleic acid molecules with precise user control over sequence content. This invention also relates to the prevention and / or removal of errors within nucleic acid molecules.

The invention provides a method for modulating gene expression by contacting a cellular system with a double-stranded ribonucleic acid molecule capable of associating with a regulatory machinery that controls transcription of one or more genes, wherein the association results in altered expression of the one or more genes. The invention is further directed to method for directing the differentiation of neuronal stem cells into neurons by contacting a cellular system with a double-stranded ribonucleic acid molecule capable of associating with a regulatory machinery that controls transcription of one or more genes involved in neuronal differentiation and directing the transcription of the one or more genes. In related embodiments, the invention provides particular compositions of double-stranded ribonucleic acid molecules as well as therapeutic and screening applications of the invention.

The invention relates to a method for synthesizing graphene oxide by an ultrasonic assistance Hummers method. The method for synthesizing the graphene oxide includes the steps as follows: firstly adding ultrasonic oscillation in low-temperature and middle-temperature reaction stages of the Hummers method to improve the intercalation efficiency and the oxidation degree of graphite oxide; secondly slowly dropping mixed liquor containing concentrated sulphuric acid into low-temperature deionized water to increase temperature when high-temperature reaction begins, thus effectively preventing intercalated materials such as sulphuric acid molecules and the like from rapidly escaping from graphite layers due to overhigh local temperature; and finally obtaining the graphite oxide through low-speed centrifugation. The method for synthesizing the graphene oxide by using the ultrasonic assistance Hummers method is convenient and fast, saves resources, and can effectively increase spacings of graphite oxide layers. The prepared graphene oxide can serve as a wild phase of a composite material and papyraceous laminated graphite oxide based composite material with high mechanical property and the like can be prepared. The reduction product graphene can be used for preparing graphene-based composite materials such as transparent electrodes, super capacitors, hydrogen storage materials, chemical / biological sensors, thin film transistors and the like.

The invention pertains to using double stranded ribonucleic acid molecules such as small interfering RNA (siRNA) molecules to target an SOD gene to interfere with gene expression and SOD protein production. Method are disclosed for inhibiting expression of a target protein in a subject with a neurological disorder by introducing a small interference ribonucleic acid (siRNA) molecule into the subject with the neurological disorder, such as amyotrophic lateral sclerosis (ALS).

The invention relates to a double-stranded RNA (dsRNA) molecule comprising between about 15 base pairs and about 40 base pairs, wherein at least one ribonucleotide of the dsRNA is a 5′-methyl-pyrimidine, and a method of using such modified dsRNA molecule to increase stability of RNA when in contact with a biological sample.

Method of deacidizing a gaseous effluent comprising acid compounds such as H2S, mercaptans, CO2, COS, SO2, CS2. An absorbent solution comprising reactive compounds with at least two amine functions is used and the following stages are carried out: a) contacting the gaseous effluent with a first fraction of the absorbent solution, so as to obtain a gaseous effluent depleted in acid compounds and a liquid effluent laden with acid compounds, b) contacting the liquid effluent obtained in stage a) with a second fraction of absorbent solution laden with molecules more acid than the acid compounds, so as to cause formation of a first liquid phase depleted in acid compounds and of a second liquid phase enriched in acid compounds and laden with more acid molecules, c) separating the first phase from the second phase, d) regenerating the second phase so as to release a gas stream rich in acid compounds and a liquid stream poor in acid compounds and laden with more acid molecules.

Short deoxyribonucleic acid molecules that are partially single-stranded, dumbbell-shaped, and covalently closed, which contain one or more unmethylated cytosine guanosine motif (CpG motif) and exhibit immunomodifying effects. Such molecules can be used for immunostimulation applications in humans or vertebrates.

The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3' terminus of one end and has a single stranded 5' overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5' overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

The invention relates to methods for chemically and physically modifying nanotubes with nucleic acid, and uses thereof. In particular, the invention relates to one or more nanotube(s) with one or more numcleic acid molecule(s) with one or more nucleic acid molecule(s) attached thereto.

The invention discloses a synthesis method of a polycarboxylic acid high-performance water reducing agent of a star-like structure, belonging to the field of a water reducing agent. According to the synthesis method, polybasic alcohol, (methyl) acrylic acid, a polyethylene glycol compound and the like are used as esterification raw materials, and the polycarboxylic acid high-performance water reducing agent of the star-like structure is prepared through a sequential esterification and polymerization method, i.e., the (methyl) acrylic acid and the polybasic alcohol are used as reactants, a star-like polymerizable active end is prepared through esterification reaction under the action of a catalyst, and then the polycarboxylic acid high-performance water reducing agent is prepared from the star-like polymerizable active end and esterification macromolecule, a molecular weight regulator and an unsaturated carboxylic acid monomer under the action of an initiator through free radical polymerization reaction. The synthesis method has the advantages of simplicity, easiness in control, high polymerization degree, low cost, no pollution, capability of realizing the polycarboxylic acid high-performance water reducing agent of the star-like structure connected with a plurality of polycarboxylic acid molecules, and more superior cement paste fluidity and holding capacity, good cement adaptability and concrete application performance compared with the conventional linear and comb-shaped polycarboxylic acid water reducing agent in the case of normal and low added amount.

The invention relates to a double-stranded RNA (dsRNA) molecule comprising between about 15 base pairs and about 40 base pairs, at least one 5′-methyl-pyrimidine and at least one 2′—O-methyl ribonucleotide, and a methods of using such modified dsRNA molecule to increase stability of a siRNA molecule when in contact with a biological sample, to reduce off-target effects and to reduce interferon responsiveness (IFN) of the siRNA molecule.

Methods, reagents and kits are provided for the production and use in detection assays of labeled nucleic acid molecules wherein a labeling molecule is attached directly to the 3′ end of the nucleic acid molecules.

The invention discloses oil-water separation copper foam and a preparation method thereof. The preparation method comprises the following steps of: selecting three-dimensional through-hole copper foam with the thickness of 1-3mm and the aperture size of 400-1500mu m, cutting the copper foam into required size, respectively washing with acetone and ethanol in an ultrasound environment, drying with nitrogen, immersing in dilute acid for treatment, then washing with a diluted alkaline solution and deionized water, and drying; then immersing in a closed 5-DEG C ammonia-water solution with the thickness of 0.1-0.5mol / L for 12-48 hours, taking out, washing with clean water and drying; finally soaking in stearaic acid modification liquid for 30-90 minutes under a 80-95 DEG C water bath condition, then washing with an ethanol solution so as to remove unabsorbed or weak unabsorbed stearaic acid molecules and other impurities on the copper foam, and finally drying to obtain the long-chain alkane molecular modified copper foam. The copper foam has a high-efficiency oil-water separation characteristic.

The invention discloses a soil restoration method based on oxygen activation of iron powder and polyphosphoric acid molecules. According to the method, by utilizing an environment-friendly low-cost oxidation reaction system formed by polyphosphoric acid, zero-valent iron and air, oxygen is activated by a Fe<2+> ligand generated by oxidizing polyphosphoric acid and the iron powder, so that the H2O2 is generated in situ and forms a Fenton reaction with Fe<2+>; and thus pollutants in the organic polluted soil are degraded. Compared with the traditional Fenton system or the similar Fenton system, polyphosphoric acid is very stable, difficult to degrade, environment-friendly and nontoxic to a human body, so that polyphosphoric acid does not generate secondary pollution as compared with other organic ligands, i.e., polyphosphoric acid is more durable in catalytic degradation effect. In addition, as the method is used, the erecting of a complicated device is not required; the restoration process is easy to operate; and the reaction can be performed by only mixing catalysts. Therefore, the dangerousness is avoided, i.e., the employing of a professional operator to perform the operation is not required.

The present invention relates to unique small ribonucleic acid molecules, for example siRNAs and miRNAs, identified and isolated using MPSS. Specifically, the invention is directed to the identification of a library of unique small RNA sequences from Arabidopsis thaliana. In another aspect, the small RNA sequences themselves are useful for performing biological functions, such as for example, RNA interference.

The invention provides a method for modulating gene expression by contacting a cellular system with a double-stranded ribonucleic acid molecule capable of associating with a regulatory machinery that controls transcription of one or more genes, wherein the association results in altered expression of the one or more genes. The invention is further directed to method for directing the differentiation of neuronal stem cells into neurons by contacting a cellular system with a double-stranded ribonucleic acid molecule capable of associating with a regulatory machinery that controls transcription of one or more genes involved in neuronal differentiation and directing the transcription of the one or more genes. In related embodiments, the invention provides particular compositions of double-stranded ribonucleic acid molecules as well as therapeutic and screening applications of the invention.

The invention relates to an early-strength ethers polycarboxylic acid water reducing agent and a preparation method thereof, belonging to the technical field of construction concrete additives. The preparation method of the early-strength ethers polycarboxylic acid water reducing agent comprises the steps: mixing and dissolving TPEG with a molecular weight of 6000 to 8000 and deionized water at the normal temperature, then adding a catalyst, then successively dropwise adding a solution A formed by mixing acrylic acid, sulphosalts unsaturated monomers and deionized water and a solution B formed by mixing an initiator, a chain transfer agent and deionized water to perform the water-based polymerization reaction, stirring after the dropwise addition, and finally adding an alkaline liquid to perform the neutralization to obtain the early-strength ethers polycarboxylic acid water reducing agent. A great amount of rigid benzene rings and sulfonate radicals are introduced into the molecular structure of the water reducing agent, so that the content of carboxylic groups having a delayed coagulation effect in the polycarboxylic acid molecules is greatly reduced, the early strength effect of the polycarboxylic acid water reducing agent molecules is greatly improved, a certain water reducing performance is achieved, and the application prospect is wide.

The invention discloses a surface modification process for improving luminance and dispersity of porous silicon. By adopting a method that chemical corrosion is combined with electrochemical corrosion, porous silicon of tidy pore structures can be prepared in a large area, subsequently the porous silicon is subjected to surface modification by using olefin and olefine acid, and Si-C bonds are introduced into the surfaces of silicon grains. The photoluminescence intensity of grains of the modified porous silicon is remarkably improved when being compared with that of the grains of porous silicon which is not subjected to surface modification; and meanwhile the luminance stability of the silicon grains in polar and non-polar solvents is greatly improved, and the phenomenon that the luminous intensity is attenuated along with the time extension is effectively reduced; and in addition, the dispersing property of the silicon grains wrapping olefine acid molecules in polar solvents is good. The surface modification process is simple, convenient to operate and applicable to production in a large scale, and has a good industrial application prospect.

The invention discloses a polyacid-based metal organic frame proton conductor material of inorganic acid molecule functionalization. Polyanions and crystal water molecules are domain-limited in pore canals of the metal organic frame, wherein the structure thereof is HAaPOM-MOF, wherein HA is one of H2SO4 or H3PO4; and POM-MOF is the polyacid-based metal organic frame, and is one of [Ag8L5](PMo12O40)(H2O)10 or [Ag8L5](PW12O40)(H2O)13, wherein L is 5-phenyl tetrazole. The invention further discloses a preparation method for the polyacid-based metal organic frame proton conductor material of the inorganic acid molecule functionalization. The preparation method comprises the following steps: firstly preparing the polyacid-based metal organic frame, uniformly mixing the prepared polyacid-based metal organic frame and HA solution, separating the material, washing, drying to obtain the polyacid-based metal organic frame proton conductor material of the inorganic acid molecule functionalization.

A sterilization germ killing comfort mask, including mask layers held in the mask with a fixed belt. There is a sterilization filter layer, an electrostatic layer, a far-infrared superfine calcium layer, and a facial protection layer from outside to inside of mask body. In use, the outside air passes through a first sterilization filter layer, the preliminary filtration sterilization filter layer removes larger grains of dust and sterilization, electrostatic layer can filter through the sterilization of dust. Superfine calcium far-infrared layer removes acidic dust, bacteria and oily molecular adsorption, and neutralize the acid molecules, sterilization and decomposition of oily molecules absorbed. Facial protection layer strengthen filtering and protection of facial skin. This mask for the various molecules in air dust filtration and sterilization, sterilization dust removal ability, remove the inclusion of peculiar smell in the air at the same time, guarantee the quality of air that users breathe, and the user to wear comfortable, strong practicability.

The invention relates to a method for preparing a slump loss-resistant type polycarboxylic acid water reducer by silanizing a terminal group of amino polyether. The method provided by the invention takes an amino polyether type compound, a silane coupling agent, an unsaturated carboxylic acid type monomer and the like as main reaction raw materials and a polycarboxylic acid water reducer is prepared through firstly hydrolyzing, substituting, then carrying out condensation, and finally copolymerizing; the method comprises the following steps: firstly hydrolyzing by a halogenated silane coupling agent; then taking the silane coupling agent and the amino polyether type compound to be subjected to substitution reaction; carrying out the condensation on a substituted product and a vinyl silane coupling agent hydrolyzed product; taking a condensed product and unsaturated polyoxyethylene ether, a molecular weight regulator and the unsaturated carboxylic acid type monomer to be subjected to free-radical copolymerization reaction under the action of an initiator to prepare the polycarboxylic acid water reducer with a plurality of branched silane modified polyether side chains. The method provided by the invention has the advantages of simple and easy-to-control process, low cost, energy saving and high efficiency, environmental protection and no pollution; dual modification of the side chains of a main chain of a polycarboxylic acid molecule is successfully realized and more excellent water reduction and slump loss resistance performance, which is better than that of a common polycarboxylic acid water reducer, is represented.

Short deoxyribonucleic acid molecules that are partially single-stranded, dumbbell shaped, and covalently closed, which contain one or more unmethylated cytosine guanosine motif (CpG motif) and exhibit immunomodifying effects. Such molecules can be used for immunostimulation applications in humans and vertebrates.

The invention provides a method for preparing a magnesium-lithium alloy surface micro-arc oxidation self-assembling super hydrophobic composite coating. The method includes: placing magnesium-lithium alloy in a stainless steel electrolytic cell, and using the magnesium-lithium alloy as anode and the stainless steel electrolytic cell as cathode, treating in a constant-current micro-arc oxidation mode for 5-10 minutes to form a micro-arc oxidation film of the magnesium-lithium alloy; and soaking the treated magnesium-lithium alloy in self-assembling solution for 6-72 hours to build the super hydrophobic composite coating. A self-assembling method is used for assembling amphiphilic organic phosphonic acid molecules on the surface of the micro-oxidation film so as to form a compact and sequential organic molecule layer and the super hydrophobic composite coating on the surface of the magnesium-lithium alloy. By the super hydrophobic composite coating, surface corrosion resistance of the magnesium-lithium alloy is improved greatly while excellent performance of the micro-arc oxidation film is kept.

The invention discloses a mixed type acidity regulator for feed additives. The mixed type acidity regulator is characterized by being prepared from the following components in parts by weight: 25-35 parts of formic acid, 15-25 parts of ammonia water, 8-15 parts of lactic acid, 5-8 parts of acetic acid, 3-5 parts of citric acid and 28-35 parts of silicon dioxide. According to the mixed type acidity regulator disclosed by the invention, firstly various acid components are added into a reaction kettle for sufficient reaction and fusion, and the fused acid components are shifted into an ion state and enter a high uniform state. The mixed type acidity regulator for the feed additives, produced in a manner, has extremely high mixing uniformity, and various acid molecules are highly dispersed, so that the situation that effective components uniformly enter feeds and then the feeds are eaten is facilitated, the situation that animals stably and uniformly digest the feeds is guaranteed, increase of culturing benefits is facilitated, and the mixed type acidity regulator is a development direction of future acidity regulators and other additives. In addition, the liquid acidifying agent disclosed by the invention is safe to use, free from toxic and side effects on the growth of the animals, free from residues, and low in corrosion to animals, human bodies and production equipment.

The invention provides a polyacid-based metal organic framework crystal material and a preparation method thereof and relates to a polyacid-based metal organic framework crystal material with nano porous channels. The invention aims at solving the problems that the polyacid-based metal organic framework material synthesized by adopting the prior art generally has a single porous channel, the finally obtained material does not have the porous channels after a polyacid molecule is immobilized, and therefore, the catalytic activity of the material is low. A chemical formula of the designed and developed flexible ligand assembled polyacid-based metal organic framework material with the nano porous channels is {[CuI(btpe)2][CuII2(H2O)2(btpe)2][BW12]40}}.2H2O. The preparation method comprises the following steps: dissolving borotungstic acid, copper acetate and 1,5-bis(triazole)-pentane into deionized water, regulating pH value, and then carrying out reaction for 3 days at the temperature of160 DEG C. By carrying out the preparation method provided by the invention, the flexible ligand assembled polyacid-based metal organic framework crystal material with the nano porous channels can beobtained.

The present invention is related to novel oral compositions comprising an irreversible gastric H+ / K+-ATPase proton pump inhibitor (PPI) as a gastric acid secretion inhibitor and one or more aliphatic carboxylic acid derivative molecules which activate parietal cells, wherein the derivatives possess delayed or sustained enhancement effect on the PPI activity compared to the non-derivatized acid molecules. The present invention further relates to a method of using such compositions to reduce gastric acid secretion in a mammal.

The present disclosure provides meroduplex (nicked or gapped) ribonucleic acid molecules (mdRNA) that decreases or silences target gene expression. An mdRNA of this disclosure comprises at least three strands that combine to form at least two non-overlapping double-stranded regions separated by a nick or gap wherein one strand is complementary to a target gene RNA. In addition, the meroduplex may have one or more modifications or substitutions, such as nucleotide base, sugar, terminal cap structure, internucleotide linkage, or any combination of such modifications. Also provided are methods of decreasing expression of a target gene in a cell or in a subject to treat a disease related to altered expression of a target gene.

The invention discloses a method for preparing graphene under the conditions of normal pressure and low temperature, which comprises the following steps that: firstly, volatile acid is used for treating graphite oxide so that volatile acid molecules are adsorbed between sheet layers of the graphite oxide, acidized graphite oxide is obtained, then, a heat treatment expansion method is adopted for preparing the graphene, experiments prove that the acidized graphite oxide generates a large amount of acid gas in the heat expansion process, and the peeling of the graphite oxide is prompted, so thegraphite oxide can generate the expansion peeling in the normal-pressure and low-temperature atmosphere, the graphene can be obtained, and in addition, the obtained graphene has high specific surfacearea. Therefore, the preparation method provided by the invention is simple, is easy to implement and overcomes the defect of vacuum or low-pressure condition limitation required during the graphene preparation in the prior art, the assurance is provided for the large-scale production of the graphene, and wide market application prospects are realized.