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Cotton GhWRKY51 transcription factor, and coding gene and application thereof

A technology of transcription factors and coding genes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem that cotton WRKY participates in abiotic stress and other problems, and achieve the effect of plant environmental adaptability and planting range expansion.

Active Publication Date: 2015-06-24
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research on the involvement of WRKY transcription factors in plant biotic stress has been gradually reported, but there are not many studies on the involvement of cotton WRKY in abiotic stress

Method used

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  • Cotton GhWRKY51 transcription factor, and coding gene and application thereof
  • Cotton GhWRKY51 transcription factor, and coding gene and application thereof
  • Cotton GhWRKY51 transcription factor, and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Cloning and expression analysis of cotton GhWRKY51 transcription factor cDNA sequence

[0036] 1. Extraction of total RNA and amplification of cotton GhWRKY51 gene EST target fragment

[0037] The cotton variety Jinmian 19 seeds were soaked in sterile water, placed in a light incubator at 25°C for 48 hours, and the photoperiod was 12 hours of light and 12 hours of darkness. After the seeds turned white, they were planted in a mixture of vermiculite and nutrient soil (1:1). The seedlings were placed in the cultivation soil and germinated in a greenhouse at a temperature of 27°C under natural light. The roots, stems, leaves, and buds, flowers, and bolls of cotton seedlings were picked during the cotton growth period, and cotton RNA was extracted using the TRIzol kit.

[0038]Referring to the cotton salt stress EST sequence, design specific primers for RT-PCR amplification to observe whether there is expression of GhWRKY51 gene in cotton leaves. The results of ...

Embodiment 2

[0070] Example 2, Obtaining and Functional Analysis of Transgenic GhWRKY51 Gene Tobacco Plants

[0071] 1. Construction of the recombinant expression vector pPZP-GhWRKY51 containing the fusion gene GhWRKY51

[0072] (1) The structure of the binary expression vector pPZP111:

[0073] The binary expression vector was provided by Wu Jiahe, a researcher at the Institute of Microbiology, Chinese Academy of Sciences. It contains the CaMV35S promoter (DE35S) with double enhancers, the Ω fragment of TMV-RNAcDNA, the BamH I-Sal I fragment from pBR322 and the Nos transcription termination Sequence composition framework for exogenous gene expression.

[0074] (2) Construction of a recombinant expression vector containing the GhWRKY51 gene

[0075] The above-mentioned GhWRKY51 gene was cloned onto the T vector, and a BamH I restriction site was added at the 5' end and a Sal I restriction site was added at the 3' end when designing the primers for the cloned gene to amplify the CDS of the ...

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Abstract

The invention relates to the technical field of cotton WRKY transcription factors and application thereof, particularly a cotton GhWRKY51 transcription factor, and a coding gene and application thereof. The cotton GhWRKY51 transcription factor is disclosed as (a) or (b): (a) protein disclosed as SEQ ID NO.2; or (b) protein variant with GhWRKY51 transcription factor functions or activity derived by substitution, deletion or / and insertion of one or more amino acids on the protein disclosed as SEQ ID NO.2. The expression of the GhWRKY51 in JA, ABA and GA is obviously enhanced; the expression variation in SA is not big; and the GhWRKY51 gene expression for drought and NaCl treatment is obviously enhanced. The overexpression of the transgenic tobacco can generate high stress tolerance.

Description

technical field [0001] The invention relates to the technical field of cotton WRKY transcription factor and its application, in particular to a cotton GhWRKY51 transcription factor, its coding gene and its application. Background technique [0002] Cotton is an important economic crop, and its fiber is an important raw material for the textile industry in the world. At the same time, cotton is also one of the important sources of oil, feed and other industrial raw materials. However, the yield and quality of cotton are often affected by insect pests, diseases and various adversities. In order to ensure food security, the cotton planting area and planting area must change. Especially in the saline-alkali land and arid land that are not conducive to planting food crops, planting cotton has become a direction of cotton research. In order to solve this problem, the research on cotton stress-resistant breeding must be strengthened. By excavating a series of genes related to s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8273
Inventor 张安红罗晓丽肖娟丽王志安
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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