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Triticum aestivum WRKY transcription factor gene and application thereof in high-temperature stress response of Arabidopsis thaliana

A technology of transcription factor and Arabidopsis thaliana, applied in wheat WRKY transcription factor gene and its application in Arabidopsis response to high temperature stress, can solve the problem of poor knowledge of wheat WRKY transcription factor, and achieve the effect of excellent gene resources

Inactive Publication Date: 2014-09-17
天津农兴源农业科技发展有限公司
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Problems solved by technology

However, little is known about the mechanism by which wheat WRKY transcription factors participate in the regulation of heat tolerance responses

Method used

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  • Triticum aestivum WRKY transcription factor gene and application thereof in high-temperature stress response of Arabidopsis thaliana
  • Triticum aestivum WRKY transcription factor gene and application thereof in high-temperature stress response of Arabidopsis thaliana
  • Triticum aestivum WRKY transcription factor gene and application thereof in high-temperature stress response of Arabidopsis thaliana

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Experimental program
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Embodiment Construction

[0014] The present invention will be described in further detail below in conjunction with specific embodiments.

[0015] 1. Acquisition of wheat WRKY transcription factor gene TaWRKY71

[0016] 1. Extraction of total RNA: Use the leaves of wheat Yangmai 158 as the test material, use the TIANGEN kit, the operation steps are as shown in the kit instructions, after obtaining the RNA solution, use agarose gel electrophoresis to identify the quality of the RNA, -80°C save.

[0017] 2. cDNA synthesis: cDNA was synthesized using Invitrogen's SuperScript TM ⅢFirst-Strand Synthesis System for RT-PCR, the specific steps are as follows:

[0018] 1) Take a 0.5ml sterilized microcentrifuge tube and add the following components respectively: 11 μL of total RNA, 1 μL of Oligo(dT) and 1 μL of 10 mM dNTP. Mix well and shake the centrifuge tube gently to make the solution concentrate to the bottom;

[0019] 2) Place the centrifuge tube in a water bath and keep warm at 65°C for 5 minutes; ...

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Abstract

The invention relates to the field of plant genetic engineering, provides a triticum aestivum WRKY transcription factor gene and further relates to application of the gene in high-temperature stress response of Arabidopsis thaliana. The nucleotide sequence of the triticum aestivum WRKY transcription factor gene is represented by SEQ ID NO: 1. Arabidopsis thaliana plants transformed with the gene have obvious phenotypic changes during the stressing of adverse situations, such as high temperature, and the gene participates in a stress-resisting defense signal transduction response process, so that excellent genetic resources are provided for the genetic improvement of triticum aestivum.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, in particular to a wheat WRKY transcription factor gene and its application in Arabidopsis response to high temperature stress. Background technique [0002] Wheat is the second largest ration crop in my country, and its stable and high yield is an important basis for ensuring national food security. Most of the wheat areas in my country often experience high temperatures above 30°C during the grain filling stage of wheat, which seriously affects the yield and quality of wheat. In addition, "high temperature forced ripening" disasters often occur in northern my country and the middle and lower reaches of the Yangtze River wheat areas. High temperature stress causes disordered growth and development of wheat, and even dry seedlings, which is one of the main natural disasters that limit the yield of wheat in my country. Transcription factors play a central regulatory role in plant gene exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 谢晓东丁博王俊斌李明陈帅君郭雨
Owner 天津农兴源农业科技发展有限公司
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