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Molecular marker of wheat scab infection-resistant gene Fhb5 and application thereof

A molecular marker, head blight technology, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of speeding up gene cloning research, large physical distance, etc., to achieve the effect of convenient, difficult and cost-saving identification

Inactive Publication Date: 2012-09-26
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, although Fhb5 has been finely mapped, the physical distance corresponding to the interval is still very large. It is necessary to develop more closely linked molecular markers to speed up the cloning research of this gene

Method used

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  • Molecular marker of wheat scab infection-resistant gene Fhb5 and application thereof
  • Molecular marker of wheat scab infection-resistant gene Fhb5 and application thereof
  • Molecular marker of wheat scab infection-resistant gene Fhb5 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0040] The molecular marker of the above-mentioned wheat head blight resistance gene Fhb5 was obtained by the following method:

[0041] (1) Wangshuibai Fhb5 near-isogenic line NMAS015 and its recurrent parent Mianyang 99-323F 2:3 Creation of populations and screening of Fhb5 segment recombinants:

[0042] (1) NMAS015(♀) and wheat variety Mianyang 99-323 hybrid F 1 , F 1 Selfing produced F containing 2816 individuals 2 group;

[0043] (2) Using the boundary markers Xgwm304 and Xgwm415 of Fhb5 ( et al.1998) in F 2 Four heterozygous individual plants recombined in this segment were screened out in the population, and the same marker was used in its F 3 Generation screening yielded 10 homozygous individuals recombined in this segment.

[0044] (2) Identification of recombinant disease-resistant phenotypes

[0045] Sow susceptible wheat grains in the field about ten days before flowering to inoculate these homozygous recombinants, and repeat once a week later. The dise...

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Abstract

The invention relates to a molecular marker of a wheat scab infection -resistant gene Fhb5 and an application thereof, belonging to the field of crop breeding. The molecular marker is either MAG8217 or MAG2462, whose genetic distance to the Fhb5 gene is 0.02 cM or 0 cM respectively. In the world, a molecular marker which is linked to the Fhb5 closestly is first acquired in the invention. Either marker is a codominant marker, which has advantages of being convenient in detection, stable for amplification, and simple in operation. The markers of the MAG8217 and the MAG2462 are used to detect Fhb5 gene, so that existence and the existing state of Fhb5 can be determined and the scab resistance of wheat can be predicted, thereby rapidly screening Fhb5-containing plants and using the plants for seed selection of disease-resistant varieties. At the same time, the molecular marker can be used in laboratory detection to avoid environmental influences on phenotypes. In the invention, using the molecular marker tightly linked to the Fhb5 can substantially save time and labor for screening disease-resistant materials, improve the prediction accuracy, and accelerate the cloning of the Fhb5 gene.

Description

technical field [0001] The invention belongs to the field of crop breeding, and relates to a molecular marker of wheat scab infection resistance gene Fhb5 and its application. technical background [0002] Wheat is one of the most important food crops in the world, providing about 20% of human energy. Many biotic and abiotic stresses directly affect the yield and quality of wheat in wheat production. Scab is one of the most important biological adversities, which seriously affects the quality and yield of wheat. The breeding and application of disease-resistant varieties is the most economical and effective method to control scab. The discovery of disease-resistant genes is the premise and foundation of disease-resistant breeding, and the development of molecular markers closely linked to disease-resistant genes is the most important way to apply disease-resistant genes. key. [0003] There are five main types of resistance to scab in wheat (Mesterhazy 1995), of which res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 马正强薛树林李国强周岩
Owner NANJING AGRICULTURAL UNIVERSITY
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