30results about How to "Increase resistance level" patented technology

The invention discloses a breeding method for rice with high and stable resistance to rice blast. By means of high-density direct seeding of F2 in the earlier stage, construction of rice blast resistant hybridized combinations, induction of rice blast with an environmental induction method and quantitative seeding of F3, the hybridized combination with rice blast resistant potential is effectivelyscreened, screening workload is reduced, and breeding effect is improved; furthermore, seedling cultivation density has no difference in the seedling stage, influence of cultivation density on rice blast induction is reduced, and single-plant rice blast resistant breeding effect is improved; by the aid of all-region planting, induction and screening of the rice blast resistant combination, loss of horizontal resistance resource of rice blast is prevented, and the combination has general and stable resistance to physiological strains; resistance level of the selected rice combination to localdominant physiological strains is improved through vertical disease resistance screening, and characteristics of stable resistance is further improved under inoculation identification of the local dominant physiological strains of rice blast; besides, under the action of medium optimization, callus differentiation and cell division are effectively promoted, and anther-culture breeding efficiency is improved.

The invention discloses a molecular marking method for tight interlocking of new gene Wbph 9 (t) for resisting rice sogatella furcifera, and application thereof. Through genetic linkage analysis, a variety F41 of the high resistant sogatella furcifera and a variety laurel 1025 of high sensitive sogatella furcifera to obtain F2 which consists of 3 family groups and recombinant inbred line groups; a rice resistant sogatella furcifera gene Wbph 9 (t) on the #6 chromosome of the rice is accurately positioned; the gene is positioned in a 120kb zone between the molecular marker MG11 and MG 32; the selecting efficiency of 1 molecular market MG21 of the zone for the antigen single plant is about 98%; the molecular market tightly interlocked by Wbph 9 (t) is applied to detect if the insect resisting variety K41 and its derivative variety (series) contains the gene; thus new material for resisting sogatella furcifera can be selected. The invention is applied to the molecular marking of the rice sogatella furcifera resistance so as to assist the seed breeding and pyramiding breeding, so as to perform the gene type selection on the low generation of breeding material in seedling period; thus the breeding efficiency is improved, and the breeding progress is accelerated.

The invention discloses mutant protein capable of being combined with nisin. The protein provided by the invention is a) or b): a) the protein formed by an amino acid sequence shown by the sequence 2 in a sequence table; b) the protein formed by substituting, deleting and / or adding one or more amino acid residues in the amino acid sequence shown by the sequence 2 in a sequence table, capable of being combined with the nisin, not degrading the nisin and derived from the sequence 2. The coded gene of the protein also belongs to the protection range of the invention. By combining NSRS236A expressed in engineering bacteria and nisin molecules, the nisin concentration on the surface of the engineering bacteria is reduced, and the resistance level of the engineering bacteria against the nisin is enhanced, so that the expression level of the nisin is improved.

The invention discloses a rice WD40-rich repeat protein OsWD40‑141, its coding gene and application. The present invention first discloses the application of any of the following proteins in regulating plant bacterial blight resistance: A1) the protein whose amino acid sequence is SEQ ID No.1; A2) the amino acid shown in SEQ ID No.1 A fusion protein obtained by connecting the N-terminal or / and C-terminal tag of the sequence; A3) the amino acid sequence shown in SEQ ID No.1 through the substitution and / or deletion and / or addition of one or several amino acid residues and The proteins shown in A1) have more than 90% identity and have the same function. Further disclosed is a method for cultivating gene mutant plants with enhanced resistance to bacterial blight. The invention provides an efficient breeding method for creating bacterial blight-resistant materials based on the protein OsWD40‑141, and has important application value.

The invention discloses a method for cultivating a rice blast-resisting rice variety. The method comprises the following steps: selecting parents: verifying a rice blast resistance gene of a rice material, and combining and preparing superiority combinations according to the following rules: at least one of the parents contains the relationship of a main variety of which the accumulative promotion area is 5 million mu or more, a rice blast-resisting multi-gene pyramiding mode Pita+Pb1+Piz-t is met, and resistance genes are complementary; moderately broadening the amphilepsis distance according to a parent family tree and form difference, enabling the yield super-parent advantage of F1 to be 8 percent to 15 percent, and enabling the super / mid-parent advantages of the plant height and the sowing period of the F1 to be both smaller than 3 percent; carrying out pyramiding breeding according to a breeding goal, thus obtaining the rice blast-resisting rice variety, wherein the breeding goal at least comprises a goal of pyramiding Pita+Pb1+Piz-t and a goal of resisting neck blast. The yield and the resistance of the rice variety bred by utilizing the method are well coordinated, and a resistant spectrum and the durable resistance level of the neck blast are particularly and obviously increased.

The present invention obtains through separation sea island cotton defense response regulating genes GbRar1, GbSgt1 and GbRac1, and constructs three plant expression vectors of constitutive expression separately. Through transforming tobacco in root nodule agrobacterium process, these three kinds of defense response regulating genes are overexpressed in tobacco, and the transgenic tobacco has obviously raised tobacco red spot resistance. Obviously, these three kinds of defense response regulating genes and the technological path may be used also in disease resisting gene engineering breeding of other crops, and are significant in practice and marketing.