Mutant protein capable of being combined with nisin, and coded gene and application thereof

Abstract

The invention discloses mutant protein capable of being combined with nisin. The protein provided by the invention is a) or b): a) the protein formed by an amino acid sequence shown by the sequence 2 in a sequence table; b) the protein formed by substituting, deleting and / or adding one or more amino acid residues in the amino acid sequence shown by the sequence 2 in a sequence table, capable of being combined with the nisin, not degrading the nisin and derived from the sequence 2. The coded gene of the protein also belongs to the protection range of the invention. By combining NSRS236A expressed in engineering bacteria and nisin molecules, the nisin concentration on the surface of the engineering bacteria is reduced, and the resistance level of the engineering bacteria against the nisin is enhanced, so that the expression level of the nisin is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a mutant protein capable of binding to nisin, its coding gene and its application. Background technique [0002] Nisin (nisin) is a small peptide produced by certain Lactococcus lactis (Lactococcus lactis), which can cause food spoilage and harmful to human health Staphylococcus, Streptococcus, Mycobacterium, Listeria and many other Gram-positive bacteria It has a strong inhibitory effect and is safe and non-toxic to the human body. It is a highly efficient natural food preservative widely used in canned food, meat products, dairy products and other fields by more than 50 countries and regions in the world. At the same time, nisin also has great application potential in the fields of medicine and light industry. In recent years, with the increasing call for returning to nature, replacing traditional chemically synthesized preservatives with highly efficient and non-toxic natural pre...

AI Technical Summary

Problems solved by technology

For example, Cheigh CI et al. tried to increase the production of nisin by increasing the copy numbers of the structural gene nisZ, the regulatory gene nisKR and the immune gene nisFEG in the biosynthesis of nisin, and found that the increase of nisZ had no effect, while the increase of the copy numbers of nisKR and nisFEG To a certain extent, the yield was increased, but it affected the growth rate of the bacteria (Cheigh et al. Biotechnology Letters (2005) 27: 155-160); Wu Z et al. used the Mu transposition complex mutation technology to screen out the nisin production 12 mutants, but none of them could increase the yield and the yield of three of them was significantly reduced (Wu et al. Journal of Applied Microbiology (2009) 106: 41-48); Kim W et al. The 60kb large plasmid obtained a certain increase in yield (Kim W et al. Applied Microbiology Biotechnology (1998) 50: 429-433)

Images