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Molecular marker of brown planthopper resistance major gene qBph4(t) of paddy rice and application of molecular marker

A technology of anti-BPH and main gene, applied in the field of molecular genetics, can solve problems such as difficult to effectively introduce and aggregate insect-resistant genes, complex insect resistance identification, etc., and achieve the effects of shortening the breeding cycle, convenient identification, and clear selection targets

Active Publication Date: 2018-06-15
YANGTZE UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complexity of insect resistance identification, it is often difficult to effectively introduce and aggregate different insect resistance genes using conventional breeding methods

Method used

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  • Molecular marker of brown planthopper resistance major gene qBph4(t) of paddy rice and application of molecular marker
  • Molecular marker of brown planthopper resistance major gene qBph4(t) of paddy rice and application of molecular marker
  • Molecular marker of brown planthopper resistance major gene qBph4(t) of paddy rice and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Acquisition of Molecular Markers

[0033] (1) 9311 / ARC5984F2 population construction and phenotypic identification

[0034] (1) The previous insect resistance identification experiments showed that the farm variety ARC5984 had high resistance to the brown planthopper population collected from the paddy fields in the suburbs of Nanning. In order to find simple and effective molecular markers that are closely linked to qBph4(t), the present invention crossed the insect-susceptible variety 9311 as the female parent and the brown planthopper-resistant rice variety ARC5984 as the male parent, and then selfed the obtained F1 to construct F2 Segregated populations; each F2 individual plant obtained the corresponding F2:3 family by selfing.

[0035] (2) Insect resistance identification of parents and F2:3 families was carried out by inoculation treatment at seedling stage. In order to ensure the consistent growth of each family in the parent and F2:3 populations, al...

Embodiment 2

[0052] Example 2 Verification of Molecular Markers

[0053] 1. Materials and methods

[0054] 1.1 Materials

[0055] Negative varieties: 4 copies, susceptible varieties 9311, Nipponbare, Taichung Local No. 1, and Hebai R54, all of which are conventional rice materials preserved in our laboratory; 20 susceptible families in the offspring of the 9311×ARC5984 hybrid combination.

[0056] Positive varieties: A total of 30 insect-resistant families in the offspring of the high insect-resistant varieties ARC5984 and 9311×ARC5984 hybrids.

[0057] Molecular marker primers: RM5635; RM5742; RM5757.

[0058] 1.2 Method

[0059] Genomic DNA was extracted from leaves of rice samples by CTAB extraction, the sample DNA was amplified with primer RM5635, the amplified products were separated by 6% non-denaturing PAGE gel, and the amplified DNA bands were recorded by silver staining. (method is with embodiment 1)

[0060] 2. Results:

[0061] Using the above method, the genomic DNA of 54...

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Abstract

The invention provides a molecular marker of a brown planthopper resistance major gene qBph4(t) of paddy rice and an application of the molecular marker. Genotypes of all single strains of F2 are obtained by hybridization of insect-resistant varieties ARC5984 (male) and 9311 (female) of the paddy rice; genetic linkage analysis is performed according to a brown planthopper resistance level of an F2:3 family at the same time; the resistance major gene qBph4(t) carried by the insect-resistant variety ARC5984 is identified and located in a 6cM interval between molecular markers RM5635 and RM5742;and the resistance major gene qBph4(t) is tightly linked with the molecular marker RM5635. A molecular marker RM5757 is also linked with the gene. The molecular marker can effectively detect whether the insect-resistant variety ARC5984 and derived varieties contain a site of the resistance major gene; the selection efficiency of a brown planthopper resistance plant of the paddy rice is greatly improved; and the brown planthopper resistance variety of the paddy rice containing qBph4(t) is obtained.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to the molecular marker and application of rice main gene qBph4(t) for resistance to brown planthopper. Background technique [0002] Rice is one of the most important food crops in the world. It has been attacked by various diseases and insect pests for a long time, which has seriously threatened its production. Nilaparvata lugens belongs to the monophagous pest, which sucks the phloem sap of plants with its mouth needles, causing yellow leaves or withering of rice plants, and finally leads to yield reduction or failure. According to the China Agricultural Yearbook, rice planthoppers (mainly brown planthoppers) have occurred nationwide for many times since the 1960s, and the area of ​​damage per season has reached more than 50% of the total rice planting area, causing serious damage to rice production in my country. suffered serious losses. Since the harm of brown planthoppers mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 程玲邱永福李俊凯
Owner YANGTZE UNIVERSITY
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