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Anti-nilaparvata lugens major gene, numerator mark method and uses thereof

A major gene and molecular marker technology, applied in the field of molecular genetics, can solve problems such as changes in resistance stability, and achieve the effect of delaying loss and improving breeding efficiency.

Inactive Publication Date: 2009-04-08
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the emergence of new biotypes of N. lugens, the resistance stability controlled by major genes has changed (Kenmore et al., 1984)

Method used

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  • Anti-nilaparvata lugens major gene, numerator mark method and uses thereof
  • Anti-nilaparvata lugens major gene, numerator mark method and uses thereof
  • Anti-nilaparvata lugens major gene, numerator mark method and uses thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Materials and Methods

[0041] 1. Material selection

[0042] After the identification of insect resistance, RBPH54 was found to have strong resistance to brown planthopper. Therefore, it was used as a parent and crossed with the susceptible variety TN1. (RBPH54 and TN1 were purchased from Plant Protection Institute of Guangxi Academy of Agricultural Sciences)

[0043] 2. Identification of insect resistance

[0044] The identification method of N. lugens resistance refers to the standard seedling tray screening method SSST (IRRI, 1988) used by the International Rice Research Institute, with certain modifications. The soil used for raising seedlings is taken from the surface soil of paddy fields. After preliminary treatment, soil with uniform muddy quality is used for seedling raising. The rice seeds were soaked in water at 25°C for 24 hours, washed and germinated, and sowed in enamel seedling trays filled with the above soil. Sow 10 varieties per plate, and sow 20-3...

Embodiment 2

[0079] Example 2 Verification of molecular markers

[0080] 1. Materials and methods

[0081] 1.1 Materials

[0082] Negative varieties: no resistance gene varieties, susceptible varieties TN1

[0083] Positive variety:: contains resistance gene, is resistant variety: RBPH54 and ASD7 (purchased from Plant Protection Institute of Guangxi Academy of Agricultural Sciences)

[0084] Molecular marker primers: BYL7 and BYL8 and RM222 and RM244 were synthesized by Shanghai Sangong respectively.

[0085] 1.2 Method

[0086] Genomic DNA of rice sample was extracted by CTAB extraction method (method is the same as in Example 1). Sample DNA was amplified with primers reverse BYL7 and BYL8 and RM222 and RM244, respectively. The reaction system included 0.10 μM primers, 250 μM dNTPs, 1× PCR reaction buffer (50 mM KCl, 10 mM Tris-HCl pH8.3, 1.5 mM MgCl 2 ,), 100ng of DNA template, 1UTaq enzyme. The reaction was carried out in a Tgradient PCR machine. The reaction program was: pre-de...

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Abstract

The invention provides molecular markers of genes which resist brown plant-hoppers of rice. The invention is characterized in that each offspring is obtained by hybridizing and back crossing a resistant parent RBPH54 and a susceptible variety TN1, and resistance identification and molecular genetic linkage analysis are carried out to the strain of each offspring, thus obtaining brown plant-hoppers resistant major recessive genes, bph20(t) and bph21(t); nearest molecular markers on both sides and linked with the bph20(t) are SSR markers, BYL7 and BYL8, which are autonomously developed, and the distances between the SSR markers and the bph20(t) are 1.3cm and 1.2cm respectively; and nearest molecular markers on both sides and linked with the bph21(t) are RM222 and RM224 and the distances between the RM222 and the bph21(t) and between the RM224 and the bph21(t) are 7.9cm and 4.0cm respectively. Whether the locus of the pest-resistant genes is contained in a pest-resistant variety RBPH54 and a derived variety (species) thereof can be detected by the molecular markers linked with the genes resistant to the brown plant-hoppers, which can greatly improve the selection efficiency of the brown plant-hopper resistant rice.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a major gene locus for resistance to brown planthopper in rice and a molecular marker method thereof, which can be used in utilization of rice germplasm resources resistant to brown planthopper, selection of varieties, identification of insect resistance, and the like. technical background [0002] Brown planthopper is one of the most serious pests in rice production areas (Cheng et al., 2003; Pathak, 1972; Dyck et al., 1979; Sogawa, 1982). Practice has proved that using variety resistance is one of the best ways to control brown planthopper damage (Pathak, 1969; Sogawa, 1982). Therefore, discovering and identifying new brown planthopper resistance genes and cultivating rice varieties with durable resistance are the key to using variety resistance to prevent and control brown planthopper damage. Fine mapping of resistance genes is the basic premise of using resistance genes. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/29
Inventor 杨朗李容柏李杨瑞韦素美黄大辉刘驰黄立飞
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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