56results about How to "Shortened childhood" patented technology

The present invention discloses a cultivating method with Carya cathayensis stock as scion, the procedure of which is to establish a seedling nursery of Carya cathayensis stock; the Platycarya strobilacea, Carya cathayensis, American Carya cathayensis or Hu nan Carya cathayensis are adopted as the stock, and the growing branch of the bearing tree of Carya cathayensis is adopted as the breeding mother tree for scion and cutting; the bed seedling of Carya cathayensis or the Carya cathayensis with the diameter at ground level is equal to or larger than one point seven centimeter and transplanted at the beginning of November of the previous year before scion and the hardy Carya cathayensis with the growth of one to two years are chosen as the stock, and the spacing in the rows and spacing between rows is not less than twelve multiplied twenty five centimeters; in the period from 10th November in the previous year to 31st January then, the hardy breeding branch with full bud, short intersegment and growth of one or two years is collected from the scion mother tree in the cutting orchard as the scion, and is arranged after collection in the shadow for twelve hours to thirty six hours, and is sealed and packed in a plastic film, and is stored into a refrigerated storage at 4 DEG C; when thirty percent of stock seedling starts to sprout in the cutting orchard or in the transplanted ground, the scion is completed within two or three weeks. The technical proposal can dwarf the tree of the Carya cathayensis, shorten the childhood period and fruit ahead of time.

The invention belongs to the field of fruit tree and forest tree breeding and specifically relates to a high-scion and head-changing grafting method of carya illinoensis. The high-scion and head-changing grafting method of carya illinoensis comprises the following steps of: before sprouting of carya illinoensis in spring, keeping the trunk by about 1 m away from the ground surface and sawing off the part thereabove to form a stumping cut, and then downwards cutting open at the (symmetrical) edges of the two sides of the stumping cut by using a grafting knife, thereby forming two cuts which are 3cm long and have width determined according to the thickness of the scion, and then grafting; next, bounding with a plastic film, binding the graft with a kraft bag, wherein the diameter of the kraft bag is determined by the thickness of the stock, sawdust is filled in the kraft bag to be higher than the scion, and the humidity of the sawdust is 70%; and then tightening the upper opening of the kraft bag; and finally, covering the kraft bag with a plastic bag; one month later, removing the plastic bag is removed and tearing off the kraft bag. With the method, the high-scion grafting survival rate of carya illinoensis is higher than 98%; therefore, the high-scion grafting survival rate of carya illinoensis is effectively realized.

The invention discloses a method for shortening the juvenile period of apple hybrid seedlings, comprising the following steps of: culturing rootstock seedlings, culturing dwarf interstock seedlings, culturing apple hybrid seedlings, grafting seedlings at a high position, performing final-period management on trees and the like. According to the method, can be realized, and the semi-finished seedlings grafted at a high position after planting can blossom and fruit after growing to the 4th year, and have the fruiting plant rate up to 20% in the 5th year and up to 40% in the 6th year. Traditional seedlings need 8-10 years of juvenile period, so the juvenile period of the seedlings is shortened by 4 years; and compared with a traditional method, the plant rate after the juvenile period is greatly increased as well. According to the invention, measures of curing dwarf interstock seedlings early, sowing seedlings, taking the high-position buds of the seedlings and grafting the high-position buds to the dwarf interstock seedlings, and then planting, and other steps are adopted, and interstock grafting is performed on the apple seedlings, thus greatly shortening the juvenile period of the apple seedlings, shortening breeding process and fruiting time, increasing breeding efficiency, and saving lots of field management cost and resource investment simultaneously.

The invention discloses a seedling culturing and planting technology of carya illinoensis and relates to the field of planting of fruit trees. The planting method comprises the following steps: (1) constructing a foundation; (2) accelerating germination and breeding; (3) grafting; (4) managing grafted seedlings; and (5) carrying out field management. By reasonably selecting carya illinoensis breeding, grafting and after-survival management methods, a lot of high-quality seedlings are obtained and sufficient nutrient supply of stocks is guaranteed; the time of breeding nursery stocks through a grafting technology is greatly shortened; dwarfing and shorted juvenile phases of walnut trees are realized and the walnut trees are fruited in advance.

The invention discloses a Jiuqian plum tree engrafting and cultivating method, comprising the following steps: planting the cultivated annual bitter plum rootstock seedlings in the seedlings-raising land in rows, in each December to establish a bitter plum free-stock nursery; selecting the bearing tree vegetative-shoots of Jiuqian plum trees as the scion cultivation stock trees to establish a scion nursery; collecting the vegetative shoots on the scion stock trees as scions to engraft in the middle ten days of next February; promoting the growth and harvest of the Jiuqian plum tree using management practices, plant growth regulator, shaping and trimming. The engrafting and cultivating method can stunt the Jiuqian plum trees, reduce the juvenile phase, advance the harvest phase, increase the yield and realize the scale planting.

The invention discloses an easy and fast seedling method for hybrid pear seeds, which comprises the steps of fruit management, seed management and seedling management, wherein the fruit management is as follows: hybrid fruits can be collected in 5-10 days before morphological maturity and are aired and pre-arranged for 3-4h, and then are instantly arranged in a miniature mechanical refrigeratory for refrigeration under the temperature of 3-5 DEG C and a humidity of 85-95 percent; and the hybrid fruits can be stored until the first ten-day period of January in next year in the refrigeratory; the seed management is as follows: the hybrid fruits are delivered from the refrigeratory and are immediately cleaned and then are aired for 48h under a room temperature (0-10 DEG C); then the hybrid fruits are dipped for 0.5-1.0h by formaldehyde (formalin) 100 times liquid with a concentration of 40 percent; and then the hybrid fruits are directly sown without sand storage and lamination; and the seedling management is as follows: when the cotyledons of the seedlings are developed to two cotyledons and one core, the cotyledons are directly transplanted to a selective nursery in the current year with a permanent planting density of 2.0m*1.0m; and only the central trunks of the seedlings are maintained for extended growing so as to increase the height of crowns and try to enter the 'childhood length' of the adult stage as early as possible and shorten the childhood period.

The invention provides Gingko MADS-box transcription factor gene GbMADS9 for controlling blossoming of plants; the gene is acquired by subjecting cDNA as a template to PCR (polymerase chain reaction) amplification, wherein the cDNA is acquired by reverse transcription of total RNA of gingko female flowers. The gene has the advantages that the gene GbMADS9 is capable of controlling blossoming of plants and shortening juvenile phase of plants, and under induction of GA3, ABA, salt stress, drought stress and cold damage stress, the overexpressed gene GbMADS9 is capable of enhancing plant tolerance to osmotic stress; the gene is applicable to controlling the blossoming phase of plants.

The invention relates to a method for reducing juvenile period of hybrid tangerine, which comprises the following steps: culturing hybrid seedlings till the hybrid seedlings grow two true leaves and two stages of roots; performing high grafting of the hybrid seedlings onto tangerine branches which have closer relationships with grafts, is free from diseases and insects and 100 centimeters above the ground and have diameters more than 3 centimeters; and finally, performing final-period management such as tree shape control and girdling for promoting promoting flower-formation. When the method is used, the juvenile period of hybrid tangerine trees is reduced to about 2 years, and the juvenile period of some hybrid tangerine trees is reduced to 1 year. Compared with the conventional cultivation method of hybrid tangerine breeding seedlings, the method has the advantages of greatly reducing breeding period and accelerating the breeding of tangerine hybridization breeding.

The invention relates to a method for boosting pear hybrid seedlings to form flowers ahead of time, belonging to the field of crop breeding and cultivation. The hybrid seedlings with certain growth undergo branch drawing, shoots twisting, girdling, PP333 spraying in a single or combined manner when the new shoots of the pear trees stop growing, later, the flower beginning conditions of the plants are surveyed and statistics of the flowering rates is made. The above treatment measures can boost the hybrid seedlings to form flowers 1-2 years ahead of time, thus effectively shortening the juvenile phase of the hybrid progeny. The method has theoretical and practical significance in shortening the breeding period, reducing the land occupation period of the hybrid seedlings and labors for orchard management and improving the selecting and breeding efficiency of the new fruit tree varieties and has broad application prospect in fruit tree breeding.

The invention provides a method for grafting citrus tangerina tanaka. In the method, a nucellar seedling graft of citrus tangerina tanaka is used for grafting, a raisin tree is taken as a base stock, and citrus tangerina tanaka, sweet orange, navel orange, Minneola orange or guiwangan is taken as an intermediate stock. Through scion picking and grafting of an excellent mother plant of nucellar seedling citrus tangerina tanaka, the aim of refitting a low-yield and low-efficiency orange garden can be fulfilled quickly, a juvenile stage is shortened by 2-3 years, the quality is enhanced, the competitiveness is enhanced, and a good economic benefit is achieved.

The invention discloses a method for shortening a juvenile phase of potted commodity camellia flowers. The method comprises the steps of cutting propagation of seedlings, potting, management after potting, pot combination and management and disease and pest prevention and control. The method enables the camellia flowers to rapidly grow within a short period of time, a potted flower production period is shortened from 3-4 years of traditional grafting and cultivation to 18 months, the juvenile phase of the potted camellia flowers is remarkably shortened, the planting cost of the potted commodity camellia flowers is reduced, and meanwhile the shortcoming that pathogen spreading of cultivation soil and difficult killing of soil protozoons are adverse to export customs clearance is overcome.

The invention discloses a phalaenopsis flowering gene PhalLFY and application thereof. The sequence of the phalaenopsis flowering gene PhalLFY is as shown in the specification from the 41 base to the 1462 base of SEQ ID NO.1, and the encoded protein amino acid sequence of the gene PhalLFY is as shown as SEQ ID NO.2. The homologous gene PhalLFY of the gene LFY is cloned from phalaenopsis for the first time, and capable of affecting plant growth and development, promoting precocious flowering of plants and promoting axillary buds and lateral buds to gown into flower buds as proved by tests. Therefore, based on the phalaenopsis flowering gene PhalLFY, an effective technical means is provided for improving plant growth process through genetic engineering and regulating the flowering time; the phalaenopsis flowering gene PhalLFY has wide application prospect and high economic value.

The invention relates to a flower forcing method for passion fruit seedlings. The method includes the steps that 1, the seedlings are cultured until the age of the seedlings is seven months, and the length of seedling bodies is about two meters; 2, agriculture trimming is performed two times, and growth of first-level vines and growth of second-level vines are promoted; 3, with leaf critical withering being limit control, water treatment is performed for 6-10 days; 4, growth regulation and fertilizer application treatment of plants are performed,paclobutrazol is used for spraying for 5 days, and rapin and monopotassium phosphate are used for mixed spraying. Within 7-15 days after growth regulation and fertilizer application treatment, namely, buds start to appear from the fourth month after transplanting, normal blossoming and fruit bearing are realized, flowering is earlier by about three months than that in planting of common passion fruit seedlings, and the flower forcing method issimple, easy to implement, low in popularization cost and remarkable in economic benefit.

The invention provides a cultivating method of Shaniodendron subaequalum stock grafted seedlings. The method comprises the following steps: collecting Shaniodendron subaequalum seeds for cultivation; selecting planted seedlings, which are reserved with beds, healthily and strongly grow and have ground diameters of not less than 0.5 cm, as stocks; collecting healthy and strong mature branches with no diseases and flower buds from mother Shaniodendron subaequalum trees; shearing the branches into 5-6 cm long scions with 2-3 muds; sealing and refrigerating the scions; grating the scions by using a side grafting method; sealing and moisturizing the grafted parts by using film strips; and preventing rainwater from entering grafting incisions. The cultivating method of the Shaniodendron subaequalum stock grafted seedlings, provided by the invention, has the advantages of simplicity in operation and high grafting survival rate; meanwhile, the juvenile phase of the Shaniodendron subaequalum is shortened and the Shaniodendron subaequalum can come into flowers in advance, so that the problem that planted Shaniodendron subaequalum seedling plants grow up with too long time is solved; the scions with flower muds can come into flowers in the next year, so that the problem that the planted Shaniodendron subaequalum seedlings grow up with too long time is solved; the endangered state of wild Shaniodendron subaequalum plants is eased; and the bred seedlings have both flowering and foliage characteristics; therefore, the cultivating method of the Shaniodendron subaequalum stock grafted seedlings, provided by the invention, can be widely used for landscaping.

The invention discloses a cultivation method capable of shortening the juvenile phase of loquats. The cultivation method comprises the following steps: performing seed direct sowing seedling culture or nutritional bag seedling culture, cultivating loquat double-layer trunk tree forms, applying fertilizer, applying a composite plant growth regulator and the like. The double-layer trunk tree form is cultivated by utilizing the characteristics that the loquat has high dryness, remarkable layer property, high germinating ability and high branching ability; furthermore, the composite plant growth regulator is sprayed on new shoots, and seedlings are promoted to develop towards the reproduction and growth direction. The cultivation method has relatively good and stable effect, the juvenile phase of the loquats is kept for 2.5 to 6 years, mainly for 3.5 to 4.5 years, the juvenile phase of the loquats is shortened by 2 to 3 years, and the problem that the juvenile phase of the loquats is long is effectively solved.

The invention relates to a method for rapidly cultivating a new grape variety. The method for cultivating the new grape variety with excellent traits aims at shortening the crossbreeding time. According to the method, by combining the characteristics of high-quality fruits of a variety with Xinjiang native characteristics, namely white Munake grapes, and integrating the special rose fragrance of aMelissa grape variety and a flame seedless grape variety, the technical problems of long periodicity and low occurrence frequency of required traits by means of grape crossbreeding methods are solved, and the new grape dominant variety is invented. The method adopts a general crossing technology and comprises the technical steps of crossing, wherein a, pollen of male parents is collected, b, emasculation of female parents is conducted, and c, crossing pollination is conducted; cross ovule and seedling culture, wherein d, ovules are taken out, e, the ovules are cultured, f, the ovules are cut,g, the ovules are subjected to germination culture, and h, seedlings are cultured; propagation expanding and transplanting, wherein i, propagation expanding is conducted, j, seedling hardening is conducted, k, transplanting is conducted, and l, top grafting is conducted; fruit quality identification. The method uniquely adopts a high-nutrition seedling stage management mode, integrates a breedingmeans combining ovule rescue and top grafting and shortens the cycle of traditional grape cross breeding, and the cultivated new grape variety has target traits.

The invention discloses a sword-leaved cymbidium flowering integrator gene CeFT and application thereof. The sequence of the CeFT gene is shown in SEQ ID NO.1 from the 87th basic group to the 617th basic group, and the amino acid sequence of a protein coded by the gene is shown in SEQ ID NO.2. The invention clones the FT homologous gene (CeFT gene) from a sword-leaved cymbidium for the first time; and experiment proves that the gene can influence the growth and development process of plants, shorten the juvenile phase and urge the plants to flower ahead of time. Thus, the sword-leaved cymbidium CeFT gene disclosed by the invention provides an effective technical means for the improvement on the growth process of the plants and the control on the flowering season of the plants in gene engineering, thereby having broad application prospects and great economic values.

The invention discloses construction and optimization of a simple and efficient carya illinoinensis agrobacterium tumefaciens transformation system. The method comprises the following steps: carrying out streak culture on agrobacterium rhizogenes on a TY solid culture medium to obtain single colonies, selecting the single colonies, and culturing the single colonies in a TY liquid culture medium to obtain an activated agrobacterium rhizogenes solution; infecting hypocotyl or stems, close to seed parts, of carya illinoensis seedlingswith agrobacterium rhizogenes, wherein the positions of hypocotyls or stems, close to the seed parts, of the carya illinoensis seedlings are 1-5 cm away from the hypocotyls or stems of the roots of the seedlings; and carrying out co-culture and rooting culture until transgenic hairy roots of the carya illinoensis grow at the positions where hypocotyls or stems are infected. The seedlings are used as propagation materials for infection, and a series of tissue culture technologies such as culture of aseptic seedlings in an aseptic environment for subculture operation are not needed.

The invention discloses an apple breeding method. The method comprises the steps as follows: scions are sheared into short shoots which are 5-10 cm long and have two buds; roots of the scions are cutinto smooth long slopes about 2.5 cm long by a knife from one side where no buds exist, one side (scion ends) where the buds exist is cut into flat wedges, and the scion ends are placed in a homemadesoaking solution to be soaked for 6-8 h; dwarfed self-rooted stocks or dwarfed interstocks are selected to form self-rooted nutrient stocks by laying propagation, upper ends of the stocks are shearedinto flat openings, and cuts with depth of about 2.5-3 cm are longitudinally formed downwards from branches at about 1 / 4 positions of cut sections; scions are inserted into stock cuts, so that the scions and the stocks are closely attached without seams, layers are aligned to each other, and 0.2 cm of upper ends of scion cutting surfaces are exposed for binding. The graft survival rate reaches 95%or above, meanwhile, apple trees grow fast after grafting, crowns take shape and bear fruits early, and the juvenile period can be shortened by 2-3 years, so that better economic benefits can be created.

The invention discloses a method for shortening the juvenescent phase of fruit trees, which comprises accelerating germination, seeding, port transferring, seedling adaptation, field planting and training and pruning. Adopting the technology in the project, the peach seedling tree can pass the juvenescent phase in the very year of seeding and enter the adult vegetative phase, while the seedling trees of apple and chestnut, etc., can pass the juvenescent phase in the second year after the seedling tree seeding and enter the adult vegetative phase. Shortening the juvenescent phase of seedling trees of fruit crops has an important significance for shortening the breeding cycle of fruit trees and promoting the career development of fruit trees.

The invention relates to the field of plant cultivation, in particular to a flowering induction method for dendrobium nobile seedlings. The method adopts the combination of hormone induction, temperature and nutritional regulation, the problems are solved that the flowering period of the dendrobium nobile seedlings is long, and flowering is not uniform. By adopting suitable hormone concentration,spraying frequency, spraying time and reasonable low-temperature treatment temperature and time and by utilizing the C / N ratio and beneficial components of liquid-state carbon-contained compound fertilizer, the combination of nutrition regulation is conducted to induct flowering of the dendrobium nobile seedlings, multiple pseudo bulbs of dendrobium nobile can be inducted to flower, the initial flowering period is short, the quantity of flowers is large, flowering is regular, ornamental value is high, and the new method is provided for cultivation of competitive product dendrobium nobile.

The invention discloses a precursor gene of hickory micro159a. The sequence of the precursor gene is: (a) a nucleotide sequence shown as SEQ ID NO:1, or (b) a nucleotide sequence which has at least 70percent, at least 75 percent, at least 80 percent, at least 85 percent, at least 90 percent, at least 95 percent, at least 96 percent, at least 97 percent, at least 98 percent or at least 99 percentof homology with the nucleotide sequence shown as SEQ ID NO:1 and can be used for generating the hickory micro159a after cutting. The application of the precursor sequence of the hickory micro159a isexpected to advance the plant flowering artificially. Specifically, the application of the hickory micro159a is expected to regulate the flowering period of hickory, shorten the childhood of hickory and advance flowering and fruiting.

The present invention discloses a cultivating method with Carya cathayensis stock as scion, the procedure of which is to establish a seedling nursery of Carya cathayensis stock; the Platycarya strobilacea, Carya cathayensis, American Carya cathayensis or Hu nan Carya cathayensis are adopted as the stock, and the growing branch of the bearing tree of Carya cathayensis is adopted as the breeding mother tree for scion and cutting; the bed seedling of Carya cathayensis or the Carya cathayensis with the diameter at ground level is equal to or larger than one point seven centimeter and transplantedat the beginning of November of the previous year before scion and the hardy Carya cathayensis with the growth of one to two years are chosen as the stock, and the spacing in the rows and spacing between rows is not less than twelve multiplied twenty five centimeters; in the period from 10th November in the previous year to 31st January then, the hardy breeding branch with full bud, short intersegment and growth of one or two years is collected from the scion mother tree in the cutting orchard as the scion, and is arranged after collection in the shadow for twelve hours to thirty six hours, and is sealed and packed in a plastic film, and is stored into a refrigerated storage at 4 DEG C; when thirty percent of stock seedling starts to sprout in the cutting orchard or in the transplanted ground, the scion is completed within two or three weeks. The technical proposal can dwarf the tree of the Carya cathayensis, shorten the childhood period and fruit ahead of time.

The invention discloses an apocarya planting technology, and relates to the field of fruit tree planting. The planting method comprises the following steps of 1 base construction, 2 germination accelerating and seedling growing, 3 grafting, 4 grafted seedling management and 5 field management. By reasonably selecting the apocarya seedling growing, grafting and survival post-management methods, a large number of high-quality seedlings are obtained, sufficient nutrient supply of stock is ensured, nursery stock breeding time spent through the grafting technology is greatly shortened, dwarfing ofapocarya trees is achieved, the juvenile phase is shortened, and fruiting is achieved ahead of time.

The invention provides a method for improving passion fruit breeding efficiency, belongs to the technical field of passion fruit breeding, and aims to identify a plurality of filial generation singleplants of passion fruit plant lateral vines at the same time by utilizing a linear single-line cultivation frame, so that not only can the influence of single plant death and loss caused by infectionof stem rot in the planting and growth process of the hybrid seedling progeny be avoided, but also the juvenile phase of seedlings can be effectively shortened, the filial generation seedlings are promoted to blossom and bear fruits in advance to identify botanical traits and fruit quality performance of the filial generation, the purpose of accelerating the breeding process is achieved, the spaceof a breeding site is greatly saved, the labor cost is reduced, the passion fruit breeding efficiency is effectively improved, and technical support is provided for promoting the passion fruit breeding level in China.

A new technology for flowering promoting of kaffir lily is characterized by including the steps of preparing culture soil, selectively using fertilizer and adjusting illumination. By means of the processes of low-temperature processing, temperature varying, light increasing and the like, the juvenile phase of the kaffir lily is shortened by 2-3 years, and scape appears 2-3 times or even 4-5 timesevery year. The technology is suitable for planting for flowering promoting of kaffir lily.

The invention discloses a pear flowering regulation transcription factor PbrSPL15. A nucleotide sequence of the pear flowering regulation transcription factor PbrSPL15 is as shown in SEQ ID No. 1, orthe pear flowering regulation transcription factor PbrSPL15 has more than 90% of homology with the SEQ ID No.1, and is the DNA molecule for regulating a plant flowering related protein is encoded. Thepear flowering regulation transcription factor PbrSPL15 provided by the invention can regulate plants to bloom in advance, shorten the juvenile phase and provide new gene resources, and is beneficialto accelerating the breeding process, reducing the agricultural production cost, and improving the economic benefit.