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Sword-leaved cymbidium flowering integrator gene (CeFT gene) and application thereof

A technology that integrates genes and genes, applied in the fields of molecular biology and genetic engineering, to achieve great economic value, wide application prospects, and the effect of shortening childhood

Inactive Publication Date: 2012-03-21
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on the flowering mechanism of Jianlan and related gene cloning and function research.

Method used

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  • Sword-leaved cymbidium flowering integrator gene (CeFT gene) and application thereof
  • Sword-leaved cymbidium flowering integrator gene (CeFT gene) and application thereof
  • Sword-leaved cymbidium flowering integrator gene (CeFT gene) and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Cloning and homology analysis of Jianlan CeFT gene.

[0025] (1) The flower buds of the Jianlan variety "Jin Si Ma Wei" (C.ensifolium'Jin Si Ma Wei') were used as test materials, and the plant materials were grown under normal conditions in the greenhouse (L / D, 16h / 8h; 25-28°C) .

[0026] (2) RNA extraction: Trizol reagent (purchased from Invitrogen) was used to extract the total RNA of the test materials, and the whole operation process was strictly in accordance with the RNA extraction process description of the Trizol reagent.

[0027] (3) M-MLV reverse transcriptase (purchased from Promgra Company) was used to reverse transcribe the mRNA into the first strand of cDNA, and the method was carried out according to the reagent instructions.

[0028] (4) Cloning of the gene: using the reverse-transcribed Jianlan flower bud cDNA first strand as a template, PCR amplification was performed using primers FT-1F and FT-1R, the PCR product was recovered, sequenced, and a 20...

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Abstract

The invention discloses a sword-leaved cymbidium flowering integrator gene CeFT and application thereof. The sequence of the CeFT gene is shown in SEQ ID NO.1 from the 87th basic group to the 617th basic group, and the amino acid sequence of a protein coded by the gene is shown in SEQ ID NO.2. The invention clones the FT homologous gene (CeFT gene) from a sword-leaved cymbidium for the first time; and experiment proves that the gene can influence the growth and development process of plants, shorten the juvenile phase and urge the plants to flower ahead of time. Thus, the sword-leaved cymbidium CeFT gene disclosed by the invention provides an effective technical means for the improvement on the growth process of the plants and the control on the flowering season of the plants in gene engineering, thereby having broad application prospects and great economic values.

Description

Technical field: [0001] The invention belongs to the technical fields of molecular biology and genetic engineering, and in particular relates to a flowering integration gene-CeFT gene expressed in Jianlan, a recombinant plant expression vector containing the gene, and the CeFT gene can shorten the childhood period of plants and promote the early flowering of plants application in Background technique: [0002] FT (FLOWERING LOCUS T) gene is a key gene in the pathway of flowering regulation. It is located at the confluence point in the floral development pathway, integrates signals from different floral development pathways such as photoperiod pathway, vernalization pathway and autonomous pathway, and plays an important role in plant flower development. [0003] The FT gene of Arabidopsis thaliana belongs to the FT / TFL1 gene family, also known as the phosphatidyletha nolamine-binding protein (PEBP) gene family, which is named for its easy binding to phosphatidylethanolamine....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 张建霞曾宋君黄玮婷段俊吴坤林
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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