The invention discloses a seedling culturing method for a Chinese yew. The seedling culturing method includes the steps of seed collection, seed storage, seed processing, soil preparation and seedling, seedling management, transplantation, interplanting and the like. Firstly, seed coats of seeds are thinned through a rubbing method, then the seeds are mixed with wet sand and stored in a soil pit, in the next spring, the seeds are taken out and sequentially soaked in disinfectant and plant growth regulator, then, the seeds undergo accelerating germination in a greenhouse, and the seeds can be planted when the seeds become white. Farmyard manure is added after deep ploughing and soil preparation, the seeds are planted on a seedbed, covered by thin soil, watered and receive moisturizing processing, and 50-70% of the seeds are shaded in a seedling stage. In the process of transplantation, neutral and acidulous properly-thick soil layers with the height of 1500-3500m are selected, base fertilizer is added, roots of sprouts are soaked in wet earth containing organic fertilizer, and then the sprouts are planted in a three-dimensional growth environment in an interplanting mode, wherein the tree of overstory in the three-dimensional growth environment is pinus yunnanensis, and ground flora of the three-dimensional growth environment is fern. Tests show that according to the seedling culturing method for the Chinese yew, dormancy periods of Chinese yew seeds can be shortened greatly, bud ratios and transplantation survival rates are improved, and the Chinese yew is quick in growth, early in fruiting and suitable for being popularized and planted in a large area.