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Photoperiod-insensitivity Hd1 allele and molecular marker and application thereof

An allele and molecular marker technology, applied in the field of molecular breeding, can solve the problems of low accuracy and inconvenient distinction, and achieve the effects of stable expression of gene function, improved photosensitivity, improved regional adaptability and breeding efficiency.

Active Publication Date: 2018-12-21
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a functional marker is developed based on this differential site, the amplified fragments have only a 2bp difference, and the bands are similar and inconvenient to distinguish; if a linked marker is developed based on the adjacent region of this differential site, the selection accuracy is not high

Method used

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  • Photoperiod-insensitivity Hd1 allele and molecular marker and application thereof
  • Photoperiod-insensitivity Hd1 allele and molecular marker and application thereof
  • Photoperiod-insensitivity Hd1 allele and molecular marker and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1 Obtaining of photoperiod insensitive rice mutant material

[0040] The rice varieties of Zhendao series (Zhendao 18, Zhendao 19 and intermediate materials, etc.) were subjected to MNU (methylnitrosourea) mutagenesis. The mutagenesis method is as follows:

[0041] (1) Select a single plant that has been headed but only has a small amount of flowering at the top, preferably each single plant has about 3-4 ears;

[0042] (2) Cut off the flowering florets of the selected plants in the field in the afternoon of the first day, leave the florets that have not yet bloomed, and put them on a sign to mark them;

[0043] (3) Let the individual plant bloom on its own in the morning the next day, and after the flowering finishes in the afternoon, cut off the spikelets that have not yet bloomed, leaving only the spikelets that bloomed that day, and pruning off the tillers that are not heading;

[0044] (4) Dig out the listed plants, try to ensure the integrity of the ro...

Embodiment 2

[0050] Example 2 Acquisition of photoperiod insensitivity Hd1 allele and development of molecular markers

[0051] 1. F of Zhenjing 2400 / Jiahe 218 2 Population construction and phenotyping

[0052] (1) A hybrid F 1 , constructed the F of Zhenjing 2400 / Jiahe 218 2 Separation population, a total of 247 copies; each F 2 The corresponding Zhenjing 2400 / Jiahe 218 F 2:3 family lineage.

[0053] (2) Phenotype identification

[0054] The constructed population and family materials were planted in the variety target promotion area and Hainan respectively, the date of full ear was investigated, the sowing period (days from sowing to full ear) was calculated, and the phenotype identification was carried out, as follows:

[0055] The above-mentioned rice materials are planted twice a year. The first season is planted in the Xingxiang Experimental Base of Zhenjiang Agricultural Science Research Institute in the hilly area of ​​Jiangsu. Sowing on May 15, transplanting on June 15, sing...

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Abstract

The invention discloses a photoperiod-insensitivity Hd1 allele and a molecular marker and application thereof. Zhengeng 2400 (female) of a photoperiod-insensitivity variety and Jiahe 218 (male) of a photoperiod-sensitivity variety are hybridized to obtain the genotype of an F2 single plant, and genetic linkage analysis is carried out to judge whether photoperiod-insensitivity phenotype separationof a corresponding F2:3 family is achieved or not; a heading period photoperiod-insensitivity gene of the Zhengeng 2400 is positioned at the sixth chromosome and located between a mark RM276 and a mark I6-5; the Zhengeng 2400 and the Jiahe 218 are subjected to WGS-variation point detection and gene sequencing analysis, and the analysis discovers that 123bp is inserted at the first exon 517bp of the Hd1 allele of the Zhengeng 2400; and the molecular marker H1 is developed according to the difference for detecting whether the Zhengeng 2400 and derivative varieties thereof contain the photoperiod-insensitivity gene or not. Thus, the photosensitivity of japonica rice varieties can be improved, and the regional adaptability and breeding efficiency of the japonica rice varieties are greatly improved.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and in particular relates to the application of Hd1 allele and primer for photoperiod insensitivity at heading stage. Background technique [0002] Heading date is an important trait of rice, which determines the regional and seasonal adaptability of rice varieties; rice is a high-temperature short-day crop, and the heading date of a variety is determined by the temperature sensitivity, photosensitivity and basic nutrition of the variety. Photoperiod is Important conditions for rice growth period regulation. Japonica rice is a subspecies with a higher degree of evolution. Compared with indica rice subspecies, the heading date of most japonica rice varieties shows a strong photoperiod sensitivity, that is, the vegetative growth and reproductive growth of plants exhibit To a greater extent, the main agronomic traits will change, such as plant height, sowing period, effective panicles, total grain...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/11C07K14/415C12Q1/6895A01H5/00A01H6/46
CPCC07K14/415C12N15/8261C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 孙立亭林添资龚红兵景德道杨军余波钱华飞曾生元李闯姚维成杜灿灿胡庆峰巫章平周义文
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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