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Rice black-streaked dwarf virus (RBSDV) resistant locus qRBSDV9 of rice variety 9194 and molecular marker method thereof

A black-streaked dwarf disease and molecular marker technology, applied in the field of molecular genetics, can solve the problems of destroying ecological balance, polluting the environment, and unsatisfactory control effects, etc., achieving the effect of difficulty, clear location, and convenient identification

Active Publication Date: 2014-09-17
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no specific pesticides for this kind of disease in production. The main method is to reduce the harm of SBPH in the early stage, but the control effect is not ideal, and it pollutes the environment and destroys the ecological balance.

Method used

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  • Rice black-streaked dwarf virus (RBSDV) resistant locus qRBSDV9 of rice variety 9194 and molecular marker method thereof
  • Rice black-streaked dwarf virus (RBSDV) resistant locus qRBSDV9 of rice variety 9194 and molecular marker method thereof
  • Rice black-streaked dwarf virus (RBSDV) resistant locus qRBSDV9 of rice variety 9194 and molecular marker method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] Materials and Methods:

[0033] (1) 9194 / Su Yunuo F 2 Population construction and phenotyping

[0034] (1) Using the black-streaked dwarf resistance variety 9194 as the female parent and the black-streaked dwarf susceptibility japonica rice variety Su Yunuo as the male parent, a hybrid was prepared to construct 9194 / Su Yunuo F 2 separate groups, each F 2 A single plant obtained the corresponding 9194 / Su Yunuo F by selfing 2:3 family lineage.

[0035] (2) Phenotype identification

[0036] (2.1) Field induction identification

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Abstract

The invention relates to a rice black-streaked dwarf virus (RBSDV) resistant locus qRBSDV9 of a rice variety 9194 and a molecular marker method thereof. Four RBSDV resistant genetic loci of the RBSDV resistant variety 9194 are obtained by carrying out genetic linkage analysis on the genotype of a single plant F2 obtained by hybridizing the RBSDV resistant rice variety 9194 (male) with a susceptible variety Suyunuo (female) and the RBSDV resistance grade of a corresponding F2:3 family, wherein qRBSDV9 is between a marker RM3912 and a marker RM257. The RBSDV resistance levels of the resistant variety 9194 and derived varieties (lines) thereof can be predicated and the selection efficiency of the RBSDV resistant rice can be greatly improved by detecting whether the resistant variety 9194 and the derived varieties (lines) thereof contain the RBSDV resistance genetic loci via the molecular markers of the RBSDV resistant genes.

Description

[0001] Case Description [0002] This application is a divisional application of the Chinese invention patent application filed on 2013-03-29 with the application number 2013101097989 and the invention title is "rice variety 9194 resistant to black-streaked dwarf locus and its molecular marker method". technical field [0003] The invention belongs to the field of molecular genetics, and relates to a rice variety 9194 qRBSDV9 anti-black-streaked dwarf locus and a molecular marker method thereof. technical background [0004] Rice is an important food crop in my country. Rice black-streaked dwarf disease is a viral disease caused by rice black-streaked dwarf virus (RBSDV), which is mainly transmitted by persistent non-ovipositous means by the small planthopper. In recent years, with changes in farming systems and cultivation methods, warming of the winter climate, and large-scale promotion of susceptible varieties, the incidence of the virus-transmitting agent SBPH has incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13
Inventor 万建民刘裕强江玲孙志广胡金龙王宝祥王琦赵志刚王益华陈亮明刘世家刘喜陈赛华张文伟田云录王春明徐大勇
Owner NANJING AGRICULTURAL UNIVERSITY
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