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Method for improving quality of tobacco leaves by using blastococcus cavernae sp.

A kind of coccus and cave technology, which is applied in the field of tobacco alcoholization and fermentation, and can solve the problems such as no public report of Bacillus cavernosa.

Active Publication Date: 2018-03-06
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention isolates a kind of Bacillus cavernosa from nature for the first time, and it is identified as a new species of microorganism. At present, there is no public report about the Bacillus cavernosa and its use

Method used

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  • Method for improving quality of tobacco leaves by using blastococcus cavernae sp.
  • Method for improving quality of tobacco leaves by using blastococcus cavernae sp.
  • Method for improving quality of tobacco leaves by using blastococcus cavernae sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] ——Application of Bacillus burrowing bacteria SYSUZL-2 in improving the quality of tobacco leaves, the specific steps are as follows:

[0024] (1) Slant culture: medium: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; g; agar 15g; distilled water 1000ml, pH7.2; culture temperature is 28°C, culture time is 48h.

[0025] (2) Seed culture: pick part of the mycelium from the slope and insert it into the seed solution, and shake the flask for culture. Seed medium: dextrin 120g; soybean powder 40g; yeast extract 2g; tryptophan 0.5g; β-alanine 5g; magnesium sulfate 0.5g; ammonium phosphate 0.2g; ℃, and the incubation time was 36 hours.

[0026] (3) Fermentation culture: Inoculate into the seed medium according to 10% inoculum amount, shake flask culture. Culture medium: 10g soybean powder; 10g glucose; 3g peptone; 2.5g sodium chloride; .

[0027] (4) Take 1L of the fermentation culture solution, centri...

Embodiment 2

[0044] Repeat implementation 1, with the following differences: the treatment concentration is 20 mL per 1000 grams of shredded tobacco.

[0045] ——Chemical Composition Determination Results

[0046] The chemical composition test results of tobacco leaves are as follows (see Table 4, 5 for details). The experimental results show that, from the perspective of a single compound, obvious changes have taken place in the internal chemical composition of tobacco leaves treated with bacteria agents. Compared with the control, the content of total sugar and reducing sugar increased greatly, and the content of total nitrogen, cellulose, protein and nicotine decreased compared with the control. Sugar-to-nitrogen ratio, sugar-to-base ratio, and nitrogen-to-base ratio all improved. The systematic test data shows that all kinds of shredded tobacco treated with bacterial agents are better than the control, and each component in the shredded tobacco tends to be balanced. It is beneficial t...

Embodiment 3

[0058] Repeat implementation 1, with the following differences: the treatment concentration is 30 mL per 1000 grams of shredded tobacco.

[0059] ——Chemical Composition Determination Results

[0060] The chemical composition test results of tobacco leaves are as follows (see Table 7, 8 for details). The experimental results show that, from the perspective of a single compound, obvious changes have taken place in the internal chemical composition of tobacco leaves treated with bacteria agents. Compared with the control, the content of total sugar and reducing sugar increased greatly, and the content of total nitrogen, cellulose, protein and nicotine decreased compared with the control. Sugar-to-nitrogen ratio, sugar-to-base ratio, and nitrogen-to-base ratio all improved. The systematic test data shows that all kinds of shredded tobacco treated with bacterial agents are better than the control, and each component in the shredded tobacco tends to be balanced. It is beneficial t...

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Abstract

The invention discloses a method for improving the quality of tobacco leaves by using blastococcus cavernae sp.. The strain is classified and named as Blastococcus cavernae sp., and is preserved in the China Center for Type Culture Collection CCTCC, and the preservation number is M2016739. Microorganism property analysis and 16S rDNA gene sequence analysis show that the strain is a novel bacterium. After an SYSUZL-2 agent is sprayed to tobacco leaves, and after alcoholization fermentation, the fragrance quality of the tobacco leaves is improved, the content of reduction sugar is increased, thecontents of proteins, cellulose, total nitrogen and nicotine are reduced, the ratios of sugar to nitrogen, sugar to nicotine and nitrogen to nicotine trend are increased, chemical components of the tobacco leaves are relatively well harmonized, and the sensory quality of the tobacco leaves is also remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of tobacco alcoholization and fermentation, and in particular relates to a method for improving the quality of tobacco leaves by using a newly discovered bacterium (Bacillus burrowing bacteria). Background technique [0002] Tobacco is one of the important pillars of our country's finances and has made great contributions to our country's social and economic development. In order to promote the progress of tobacco in our country and realize the sustainable development of the cigarette industry, we must take the road of tobacco technology innovation. The quality of tobacco leaves directly affects the intrinsic taste of cigarettes. The State Tobacco Monopoly Administration clearly pointed out that it is necessary to "overcome the key technologies that affect the aroma and quality of tobacco leaves" and narrow the quality gap with foreign tobacco leaves in order to improve domestic and foreign market competiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01A24B3/12A24B5/00A24B15/28
CPCA24B3/12A24B5/00A24B15/28C12N1/20C12N1/205C12R2001/01
Inventor 陈兴段焰青刘秀明李文均党立志焦俊李智宇郭青
Owner CHINA TOBACCO YUNNAN IND
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