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Paracoccus denitrificans and method for biological denitrification with combination of mineralized bed and paracoccus denitrificans

A denitrification paracoccus and biological denitrification technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem of hindering the denitrification process of nitrate nitrogen, high total nitrogen in effluent, Problems such as high nitrate nitrogen content in effluent

Active Publication Date: 2019-11-15
LUBIN ENVIRONMENTAL PROTECTION TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since the 1990s, many scholars have started in-depth research on mineralized waste, and the results show that mineralized waste has a good treatment effect on ammonia nitrogen, but it has not solved the problem of excessive nitrate nitrogen content in the effluent, resulting in The total nitrogen in the effluent remains high
One of the reasons is that the conventional heterotrophic denitrification process needs to provide additional organic matter as carbon source and electron donor. When the carbon source in the system is insufficient and the carbon-nitrogen ratio is too low, the denitrification process of nitrate nitrogen will be hindered.

Method used

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  • Paracoccus denitrificans and method for biological denitrification with combination of mineralized bed and paracoccus denitrificans
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  • Paracoccus denitrificans and method for biological denitrification with combination of mineralized bed and paracoccus denitrificans

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The cultivation method of denitrification paracoccus TNS-1 provided by the invention is determined by the following steps:

[0038] 1. Activation of strains

[0039] Seed medium (g / L): peptone 5.0, potassium nitrate 1.0, pH 7.0. Sterilized by high pressure steam at 121°C for 20 minutes.

[0040] Denitrification medium (g / L): Na 2 S 2 o 3 ·5H 2 O 5.0, KNO 3 1.0,K 2 HPO 4 2.0, NaHCO 3 1.0, MgCl 2 ·6H 2 O 0.5, FeSO 4 ·7H 2 O 0.01, PH 8.0-8.5. Sterilized by high pressure steam at 121°C for 20min.

[0041] Take the preserved Paracoccus denitrificans (Paracoccus.denitrificans) TNS-1 slant strain, inoculate one loop into the sterilized seed medium under sterile conditions, and culture at 30°C, 120r / min shaking for 24-48h, as strains.

[0042] 2. Growth condition test

[0043] 1) Effect of different temperatures on growth

[0044] Inoculate the activated strain into the seed culture medium with an inoculum size of 1%, and culture at 30°C with 120r / min shaking...

Embodiment 2

[0053] Nitrogen removal test

[0054] 1. Medium

[0055] Primary medium (g / L): peptone 5.0, potassium nitrate 1.0, pH 7.0. Sterilized by high pressure steam at 121°C for 20 minutes.

[0056] Secondary medium (g / L): Na 2 S 2 o 3 ·5H 2 O 5.0, KNO 3 1.0,K 2 HPO 4 2.0, NaHCO 3 1.0, MgCl 2 ·6H 2 O 0.5, FeSO 4 ·7H 2 O 0.01, pH 8.0.

[0057] 2. Bacteria culture

[0058] Take the activated Paracoccus.denitrificans TNS-1 strain, inoculate 1% of the inoculum amount, aseptically inoculate into the primary medium, and culture at 30° C. and 120 r / min for 24-48 hours with shaking. Take the first-grade bacteria, transfer it to the second-grade culture medium according to the inoculation amount of 1-2%, and cultivate it statically for 5-7 days.

[0059] 3. Bacteria adsorption

[0060] Pour the expanded bacterial solution into the packing tank until it just submerges the top layer of the packing, and keep it submerged for 3-5 days until the bacteria are naturally adsorbed on...

Embodiment 3

[0072] Pilot test for denitrification

[0073] 1. Medium

[0074] Primary medium (g / L): peptone 5.0, potassium nitrate 1.0, pH 7.0. Sterilized by high pressure steam at 121°C for 20 minutes.

[0075] Secondary medium (g / L): Na 2 S 2 o 3 ·5H 2 O 5.0, KNO 3 1.0,K 2 HPO 4 2.0, NaHCO 3 1.0, MgCl 2 ·6H 2 O 0.5, FeSO 4 ·7H 2 O 0.01, pH 8.0.

[0076] 2. Bacteria culture

[0077] Take the activated Paracoccus.denitrificans TNS-1 strain, inoculate 1% of the inoculation amount, aseptically inoculate into the primary medium, and culture at 30° C. with shaking at 120 r / min for 24-48 hours. Take the first-grade bacteria, transfer it to the second-grade culture medium according to the inoculation amount of 1-2%, and cultivate it statically for 5-7 days.

[0078] 3. Bacteria adsorption

[0079] Pour the expanded bacterial solution into the packing tank until it just submerges the top layer of the packing, and keep it submerged for 3-5 days until the bacteria are naturally ...

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Abstract

The invention provides paracoccus denitrificans and method for biological denitrification with combination of mineralized bed and paracoccus denitrificans. The paracoccus denitrificans is autotrophicdenitrification paracoccus denitrificans TNS-1, mineralized garbage is selected as a main ingredient of a packed bed, waste is recycled, the biologic enhancement effect is adopted for total nitrogen removal, and the wastewater nitrogen removal method is environmentally friendly, free of secondary contamination, low in treatment cost, low in energy consumption and low in carbon.

Description

technical field [0001] The invention relates to a denitrifying paracoccus, and also relates to a method for biological denitrification by using the denitrifying paracoccus in combination with a mineralized bed, which belongs to the field of sewage treatment. Background technique [0002] Mineralized waste refers to that after several years of degradation in the landfill, the easily degradable substances are completely or nearly completely degraded, basically reaching a stable state, the surface settlement is very small, and the amount of naturally produced leachate and gas is very little or not. produced, the mass fraction of biodegradable substances dropped below 3%. In terms of appearance composition, except for large-grained inorganic substances such as plastics, glass, stones, and animal bones, and refractory organic substances, the rest are black dispersed granular soil-like substances with uniform texture. Mineralized waste has a good porous structure, a huge surface ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/00C12R1/01C02F101/16
CPCC02F3/34C02F2003/003C02F2003/001C02F2101/16C02F2301/10C02F2209/06C02F2209/02C12R2001/01C12N1/205Y02W10/10
Inventor 徐有权刘志鹏赵由才刘德滨
Owner LUBIN ENVIRONMENTAL PROTECTION TECH SHANGHAI
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