The invention discloses a
molecular breeding method for improving
cold resistance of rice. The method comprises the steps that a single
chromosome segment substitution line is established by taking a rice line which is weak in
cold resistance as a recurrent parent and taking a rice line which is strong in
cold resistance as a cool-resistant
gene donor; multi-environment multi-repeat cold resistance detection at an object
growth development stage is conducted, and the single
chromosome segment substitution line with stable cold-resisting QTL is screened; hybridization is conducted, molecular markers closely linked with the cold-resisting QTL are utilized for assisting in selection, and a
polymerization line with different
gene locus cold-resisting QTL is obtained; cold resistance evaluation at the object
growth development stage is conducted on the
polymerization line, and an excellent line of which the cold resistance is significantly improved is obtained through screening. According to the
molecular breeding method, identification and selection on the cold resistance are simple, rapid and economical, the excellent line which is excellent in genetic background and significantly enhanced in cold resistance can be rapidly obtained, and the breeding efficiency and accuracy are improved.