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40 results about "Haynaldia villosa" patented technology
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Haynaldia villosa (L.) Schur; Pseudosecale villosum (L.) Degen; ... Dasypyrum villosum is a species of annual grass in the Poaceae family, found in It is native to eastern and southern Europe and Western Asia from the Balearic Islands to Turkmenistan, including in the Mediterranean and the Caucasus regions.
Haynaldia villosa's 6VS chromosome specific molecular marker 6VS-BH1 and application thereof
InactiveCN104877996AStrong brightnessWide range of annealing temperaturesMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyMarker-assisted selection
The invention relates to a haynaldia villosa's 6VS chromosome specific molecular marker 6VS-BH1 and an application thereof, and belongs to the technical fields of molecular biology and genetic breeding science. The labeled primer 6VS-BH1 is developed on the basis of wheat-brachypodium distachyon comparative genomics means, and the specific sequence is 6VS-BH1F, as shown in SEQ ID NO.1, and 6VS-BH1R, as shown in SEQ ID NO.2. The molecular marker 6VS-BH1 has the advantages of wide annealing temperature range (60-66 DEG C), good stability, strong product brightness, high resolution and the like. The molecular marker 6VS-BH1, as a co-dominant marker, not only can be used for effectively tracing haynaldia villosa's 6VS chromosome in wheat background, but also can be used for distinguishing a homozygote and heterozygote and for distinguishing a Pm21 gene carried translocation line T6VS.6AL and a PmV gene carried translocation line T6VS.6DL. Therefore, the molecular marker 6VS-BH1 disclosed by the invention has an important practical value in the molecular marker-assisted selection breeding of wheat powdery mildew and the pyramiding breeding of Pm21 gene and PmV gene.
Owner:JIANGSU UNIV
Haynaldia villosa agglutinin receptor-like kinase gene and expression vector and application
ActiveCN105821055AIncrease resistanceImprove powdery mildew resistanceTransferasesFermentationTriticeaeGene conversion
The invention discloses a tufting wheat lectin receptor kinase gene, its expression carrier and application, and belongs to the field of genetic engineering. The cDNA sequence of Hv-LecRK is SEQ ID NO.1 and the encoded amino acid sequence is SEQ ID NO.2. The gene comes from diploid W. villosa (Haynaldia villosa, L., 2n=2x=14, genome VV), and can interact with powdery mildew resistance-related gene Hv-CMPG, and is resistant to powdery mildew in diploid W. villosa Enhanced expression induced by powdery mildew. The gene was transformed into the susceptible wheat variety Yangmai 158 by transient expression, and the results showed that the overexpression of Hv-LecRK gene could reduce the haustoria index of Yangmai 158. Therefore, Hv-LecRK is expected to be used in genetic engineering breeding, and introducing it into wheat varieties susceptible to powdery mildew is expected to improve the powdery mildew resistance of wheat.
Owner:王秀娥 +2
NLR gene NLR1-V, expression vector thereof and application of NLR gene NLR1-V and expression vector thereof
ActiveCN106754960AIncrease resistancePowdery mildew resistantPlant peptidesFermentationBiotechnologyTriticeae
The invention belongs to the field of gene engineering and discloses an NLR gene NLR1-V, an expression vector thereof and application of the NLR gene NLR1-V and the expression vector thereof. A cDNA sequence of the gene NLR1-V with an NLR structural domain is SEQ ID NO.1, and an amino acid sequence encoded by the gene is SEQ ID NO.2. The gene comes from a 6VS chromosome arm of a wheat-haynaldia villosa translocation line 6VS / 6AL Nannong 9918. Inducible expression of NLR1-V by powdery mildew in the powdery mildew resisting wheat variety Nannong 9918 is enhanced. The gene is converted into a susceptible wheat variety Yangmai 158 through transient expression, and the result shows that overexpression of NLR1-V can reduce the haustorium index of the Yangmai 158. Thus, NLR1-V is expected to be used for gene engineering breeding and is expected to improve the powdery mildew resistance of the wheat when introduced to the powdery mildew susceptible wheat variety.
Owner:NANJING AGRICULTURAL UNIVERSITY
Molecular markers having haynaldia villosa 6VS chromosome specificity and use thereof
InactiveCN102943073AStrong specificityEasy to identifyMicrobiological testing/measurementDNA/RNA fragmentationTriticella flavaMarker-assisted selection
The invention discloses molecular markers having haynaldia villosa 6VS chromosome specificity and a use thereof, and relates to the technical field of molecular biology and genetic breeding science. The molecular markers can specifically track a haynaldia villosa 6VS chromosome. The molecular markers as primers are designed according to an EST sequence BE585684 of a short arm of a wheat sixth-part homologous group and have sequences of 6VS-SSDF: 5'-CCAACTCTAGCTGACCGCAGACTA-3' and 6VS-SSDR: 5'-ATTCCATGGTGTAATAGCTCCAACTAC-3'. Through PCR, the molecular markers are amplified into a specific DNA band having the length of 350bp. The molecular markers can fast and effectively track a haynaldia villosa 6VS chromosome and wheat powdery mildew resistance controlled by the haynaldia villosa 6VS chromosome, and has strong practical values in wheat powdery mildew-resistant molecular marker-assistant selective breeding of wheat.
Owner:JIANGSU UNIV
Haynaldia villosa powdery mildew resistant gene DvRGA-1, DvRGA-2 and application thereof
The invention discloses a haynaldia villosa powdery mildew resistant gene DvRGA-1, DvRGA-2 and the application thereof, and belongs to the field of gene engineering. The genomic DNA sequence of haynaldia villosa DvRGA-1 is SEQ ID NO. 1, the cDNA sequence is SEQ ID NO. 2, and the amino acid sequence is SEQ ID NO. 3. The genomic DNA sequence of DvRGA-2 is SEQ ID No. 4, the cDNA sequence is SEQ ID NO. 5, and the amino acid sequence is SEQ ID NO. 6. The DvRGA-1 is located at the Pm21 locus of haynaldia villosa powdery mildew resistant gene. Gene silencing analysis shows that the silencing of DvRGA-1 and DvRGA-2 can lead to complete powdery mildew of disease-resistant wheat; the analysis of transient expression shows that DvRGA-1 and DvRGA-2 can significantly reduce the haustorium index of powdery mildew of susceptible wheat. Therefore, the haynaldia villosa DvRGA-1 and DvRGA-2 genes and the coded proteins have important application value in wheat powdery mildew resistant breeding.
Owner:JIANGSU UNIV
Method for rapidly identifying H. villosa chromosomes in wheat background
ActiveCN106967809AOvercoming Uncertainty About WheatOvercome the shortcoming of whether the chromosome of T. villosa has been translocatedMicrobiological testing/measurementDNA/RNA fragmentationNucleotideTriticeae
The invention provides a method for rapidly identifying H. villosa chromosomes in a wheat background. According to the method, two oligonucleotide probes are firstly provided, the nucleotide sequences of the oligonucleotide probes are respectively shown as SEQ ID NO.1-2, the two probes are mixed and combine with an ND-FISH technology and can be used for identifying the H. villosa chromosomes in a wheat and haynaldia villosa hybridization generation, 7 H. villosa chromosomes are accurately identified while wheat chromosomes and the H. villosa chromosomes can be clearly distinguished, and wheat-haynaldia villosa translocated chromosomes can be identified. By adopting the method, the shortcomings of a GISH technology and an existing ND-FISH technology can be overcome, and the effects of the GISH technology and the existing ND-FISH technology can be also achieved. The method facilitates identification of the wheat-haynaldia villosa translocated chromosomes and has the very good prospect in the field of wheat breeding.
Owner:SICHUAN AGRI UNIV
KASP molecular marker of wheat powdery mildew resistance gene Pm21 derived from haynaldia villosa and application of KASP molecular marker
ActiveCN111235295AImprove transgenic utilizationQuick checkMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyNucleotide
The present invention discloses a KASP molecular marker of a wheat powdery mildew resistance gene Pm21 derived from haynaldia villosa and an application of the KASP molecular marker. The KASP molecular marker is KASP-Pm21 and primers of the KASP molecular marker KASP-Pm21 comprise a haynaldia villosa allelotype upstream primer KASP-Pm21-F, a wheat allelotype upstream primer KASP-Pm21-H and a shared downstream primer KASP-Pm21-C; a nucleotide sequence of the haynaldia villosa allelotype upstream primer KASP-Pm21-F is shown in SEQ ID NO:1; a nucleotide sequence of the wheat allelotype upstream primer KASP-Pm21-H is shown in SEQ ID NO:2; and a nucleoside sequence of the shared downstream primer KASP-Pm21-C is shown in SEQ ID NO:3. The provided KASP molecular marker KASP-Pm21 of the wheat powdery mildew resistance gene Pm21 derived from the haynaldia villosa can realize efficient tracking and detection of the Pm21 gene and is applied to molecular marker assisted selection breeding of the wheat powdery mildew resistance Pm21 gene.
Owner:YANTAI UNIV
Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker
The invention discloses a set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and a molecular marker. The set reagent is composed of A-P and at lease one of B-P, C-P, D-P and E-P; the A-P is composed of two single-stranded DNA shown in a sequence 1 and a sequence 2 in a sequence list; the B-P is composed of two single-stranded DNA shown in a sequence 3 and a sequence 4 in the sequence list; the C-P is composed of two single-stranded DNA shown in a sequence 5 and a sequence 6 in the sequence list; the D-P is composed of two single-stranded DNA shown in a sequence 7 and a sequence 8 in the sequence list; the E-P is composed of two single-stranded DNA shown in a sequence 9 and a sequence 10 in the sequence list. The set reagent and a method can be used for screening a wheat cross population and identifying and assistantly breeding a new wheat line or variety with the excellent property (such as the powdery mildew resistance) of haynaldia villosa.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Molecular marker 6VS-LEM with specificity for haynaldia villosa 6VS chromosomes and application thereof
InactiveCN102864142AStrong specificityEasy to identifyMicrobiological testing/measurementDNA/RNA fragmentationTriticella flavaMarker-assisted selection
The invention discloses a molecular marker 6VS-LEM for specifically tracking haynaldia villosa 6VS chromosomes and application of the molecular marker 6VS-LEM, and relates to the technical fields of molecular biology and genetic breeding science. A marking primer is designed according to wheat EST sequence BE404563 capable of being electronically located at slender false brome grass No.3 chromosome short arms, a particular sequence is 6VS-LEMF: 5'-CCAGGAGGGCCTCCAGGA-3' and 6VS-LEMR: 5'-CTGGGAGCTCCTGCTGCGT-3', a 350bp specific deoxyribonucleic acid (DNA) stripe can be amplified when the marking primer is used for polymerase chain reaction (PCR), the haynaldia villosa 6VS chromosomes and wheat powdery mildew resisting characters controlled by the haynaldia villosa 6VS chromosomes can be rapidly and effectively tracked, and the molecular marker 6VS-LEM has stronger practical value on wheat powdery mildew resisting marker-assisted selection breeding.
Owner:JIANGSU UNIV
Chloroplast locating gene ToxABP1-V and its application
The invention discloses a chloroplast locating gene ToxABP1-V and its application. The cDNA sequence of ToxABP1-V is SEQ ID No. 1, and an amino acid sequence coded by ToxABP1-V is SEQ ID No. 3. The gene is derived from diploid Haynaldia villosa, can interact with the powdery mildew resistance-related gene CMPG1-V, and undergoes down-regulated expression in the powdery-mildew-resistant diploid Haynaldia villosa under the induction of powdery mildew. The RNAi silencing vector pWMB006-ToxABP1 of the gene is used for transforming the susceptible wheat variety Yangmai 158 by using single-cell transient silencing techniques, and results show that transient silencing of ToxABP1 can reduce the haustorium index of Yangmai 158. Three positive RNAi transgenic plants are obtained by using transgenic technology, and the plants show moderate and high resistance to powdery mildew of wheat. Therefore, ToxABP1-V is expected to be used for genetic engineering breeding.
Owner:NANJING AGRICULTURAL UNIVERSITY
Nucleic acid probes for identifying haynaldia villosa chromosomes and application thereof
InactiveCN106350594APromote diffuse distributionFast and more accurate identificationMicrobiological testing/measurementDNA/RNA fragmentationNucleic Acid ProbesHaynaldia villosa
The invention provides a pair of oligonucleotides and nucleic acid probes prepared from the same. The two nucleic acid probes can form better dispersive distribution on most of haynaldia villosa chromosomes, and by combining the nucleic acid probes with known oligomeric nucleic acid probes Oligo-pTa535, the haynaldia villosa chromosomes and / or chromosome segments in the genetic background of wheat can be identified quickly and accurately. The technology has the advantages of time saving, labor saving, low cost and capability of reaching a similar GISH (genomic in situ hybridization) effect.
Owner:SAAS BIOTECH & NUCLEAR TECH RES INST
Molecular marker of powdery mildew resistance gene Pm12 of aegilops speotoides and use of molecular marker
ActiveCN112301142AStrong specificityImprove detection accuracyMicrobiological testing/measurementDNA/RNA fragmentationAegilopsErysiphe sp.
The present invention discloses 3 molecular markers (6SS-1 to 6SS-3) for specifically tracking 6SS chromosomes of aegilops speotoides and a use method of the molecular markers, and relates to the technical field of molecular biology and genetic breeding science. Sequences of primers of the 6SS-1 marker are shown as SEQ ID NO.1 and SEQ ID NO.2, sequences of primers of the 6SS-2 marker are shown asSEQ ID NO.3 and SEQ ID NO.4, and sequences of primers of the 6SS-3 marker are shown as SEQ ID NO.5 and SEQ ID NO.6. The molecular markers can track the Pm12 gene from the aegilops speotoides, also cantrack a Pm21 gene from haynaldia villosa, can simultaneously distinguish and track the Pm12 gene and the Pm21 gene in a pyramiding breeding material, and have important practical value in assistant selection breeding and pyramiding breeding of the two genes.
Owner:CROP RES INST SHANDONG ACAD OF AGRI SCI +1
Specific codominant molecular marker of haynaldia villosa 4VL chromosome as well as primers and application of molecular marker
InactiveCN107326070AStable amplificationImprove accuracyMicrobiological testing/measurementDNA/RNA fragmentationMarker-assisted selectionGermplasm
The invention discloses a specific codominant molecular marker of a haynaldia villosa 4VL chromosome as well as primers and an application of the molecular marker and belongs to the biotechnology field. The invention firstly discloses a molecular marker CINAU321 capable of tracking the haynaldia villosa 4VL chromosome. In a material containing the haynaldia villosa 4VL chromosome, marker primers CINAU321F / CINAU321R can amplify about 1,150 bp products, and the pair of primers can specifically track the haynaldia villosa 4VL chromosome. A disclosed haynaldia villosa T4DS.4VL homozygous compensatory translocation line can provide germplasm resources for mining of excellent genes of the haynaldia villosa 4VL chromosome; the specific molecular marker capable of specifically tracking the haynaldia villosa 4VL chromosome can be used for molecular marker assisted selective breeding.
Owner:NANJING AGRICULTURAL UNIVERSITY
Breeding method using multispikelet germplasm NAU422 for improvement of wheat yield
ActiveCN104756876AGenetic stabilityMicrobiological testing/measurementClimate change adaptationAgricultural scienceGermplasm
The invention discloses a breeding method using multispikelet germplasm NAU422 for improvement of wheat yield. Common wheat Chinese spring-haynaldia villosa T2VS.2DL translocation line NAU422 is compensatory whole arm translocation line, is stable in inheritance, carries excoemum morphological marker Bgr-V1, and by use for molecular breeding, spikelet number and number of grains per panicle can be significantly improved, and high-yielding new wheat line is easy to breed.
Owner:NANJING AGRICULTURAL UNIVERSITY
Molecular markers linked with disease resistance gene Pm21 of wheat and application of molecular markers in breeding
ActiveCN107699630AImprove breeding efficiency for disease resistanceImprove breeding efficiencyMicrobiological testing/measurementPlant cellsBiotechnologyDisease
The invention discloses molecular markers linked with a disease resistance gene Pm21 of wheat and application of the molecular markers in breeding. 25 molecular markers are derived from Triticum aestivum-Haynaldia villosa translocation line NAU427 of the powdery mildew resisting gene Pm21, are tightly linked with the gene Pm21 and can be used for assistant selection of the molecular markers; and selection efficiency and accuracy of disease resisting plants are improved.
Owner:NANJING AGRICULTURAL UNIVERSITY +1
Specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus
ActiveCN103233006AStable resistanceImprove use valueMicrobiological testing/measurementDNA/RNA fragmentationTriticeaeHaynaldia villosa
The invention discloses specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus and belongs to the field of biotechnology. The invention firstly discloses two molecular markers CINAU66 and CINAU295 which are capable of tracking the haynaldia villosa 4VS chromosome. In materials containing the haynaldia villosa 4VS chromosome, marker primers CINAU66F / CINAU66R are capable of amplifying about 950bp (base pair) of product; and marker primers CINAU295F / CINAU295R are capable of amplifying about 380bp of product; and the primers are capable of tracking the haynaldia villosa 4VS chromosome. The haynaldia villosa 4VS chromosome capable of resisting the wheat yellow mosaic virus, disclosed by the invention, is capable of proving new gene resources for the breeding of yellow mosaic virus resisting wheat; and the specific molecular markers capable of specifically tracking the haynaldia villosa 4VS chromosome can be used for molecular marker-assisted selective breeding.
Owner:NANJING AGRICULTURAL UNIVERSITY
Haynaldia villosa mitogen activated protein kinase gene, and expression vector and application thereof
ActiveCN104774854AIncrease resistanceTransferasesFermentationTriticella flavaMitogen activate protein
The invention discloses a Haynaldia villosa mitogen activated protein kinase gene Hv-MPK12 and a protein encoded thereby, and belongs to the field of gene engineering. The cDNA sequence of the Hv-MPK12 is represented by SEQ ID NO.1, and the amino acid sequence encoded thereby is represented by SEQ ID NO.2. The gene is from Haynaldia villosa L. (2n=2x=14, genome VV), and powdery mildew fungus induced expression enhancement of the gene in powdery mildew-resistant Haynaldia villosa L. (2n=2x=14, genome VV) is realized. The gene is transformed to an infected wheat species Yangmai 158, and a result shows that the overexpression of the Hv-MPK12 gene can enhance the wheat powdery mildew resistance of the Yangmai 158. The Hv-MPK12 is expected to be used in gene engineering breeding, and can be introduced in to wheat species susceptible to powdery mildew in order to possibly improve the powdery mildew resistance of wheat.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method of acquiring haynaldia villosa homozygous regeneration plant through anther culture
InactiveCN107711504ASolve the problem of weak growthHigh activityHorticulture methodsPlant tissue cultureGenomic sequencingTriticeae
The invention relates to the field of plant biotechnology, in particular to a method of acquiring a haynaldia villosa homozygous regeneration plant through anther culture. The invention also providesan improved sound seedling and rooting culture medium for the anther culture method. By adopting the method provided by the invention, the anther separation activity and the induced reaction are effectively improved from the source by determining the haynaldia villosa drawing critical period, and the problem of weak growth of a regeneration plantlet is solved through sound seedling and hardening-seedling key measures. The invention establishes a set of simple, rapid and stable regeneration system with anther in vitro, haynaldia villosa homozygous regeneration plant seeds can be acquired withinhalf an year by utilizing a program of the invention, so that the hybridization problem caused by haynaldia villosa cross pollination can be effectively solved, improvement of study on a haynaldia villosa genetic map and genome sequencing is facilitated, the anther culture efficiency of wheat distant hybrids can be improved at the same time, and application of haynaldia villosa wild baluable generesources in wheat genetic improvement is accelerated.
Owner:SHANGHAI ACAD OF AGRI SCI
Primers, detection method and application for diploid Haynaldia villosa 3V chromosome specific KASP marker detection
ActiveCN112410451ARapid and efficient high-throughput identificationFast and effective trackingMicrobiological testing/measurementAgainst vector-borne diseasesNucleotideGenetics
The invention discloses primers, a detection method and application for diploid Haynaldia villosa 3V chromosome specific KASP marker detection. Nucleotide sequences of the primers for Haynaldia villosa 3V chromosome specific KASP marker detection are: F1: 5'-GAAGGTGACAAG TTCATGAGCTGAGCTGTCGAGCTAAGCTA-3'; F2: 5'-GAAGGTCGAGTCAGGACGATTGTCGAGGAGCTGTCAGCTGAGCTGAGCTCTCCTC-3'; and R: 5'-TGGAGCAGCGAGGTGTGTGA-3'. By utilizing the marker disclosed by the invention, the Haynaldia villosa 3V chromosome can be efficiently detected in a common wheat CS background, and a foundation is provided for applying excellent traits on the 3V chromosome in wheat breeding.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +2
Haynaldia villosa calmodulin interacting protein kinase gene and expression vector and application thereof
The invention discloses a haynaldia villosa calmodulin interacting protein kinase gene and expression vector and application thereof, belonging to the field of gene engineering. The cDNA sequence of HvCIPK1 is SEQ ID NO.1, and the encoded amino acid sequence thereof is SEQ ID No.2. The gene is reported in the haynaldia villosa for the first time, expression is up-regulated due to the induction of powdery mildew germ, and overexpression of the gene in winnow wheat 158 of wheat variety resisting powdery mildew can improve the resistance of the winnow wheat 158 to powdery mildew germ. HvCIPK1 is an important element in disease resistant signal transduction pathway of haynaldia villosa and can be guided into wheat variety resisting powdery mildew, so that the resistance to powdery mildew is improved; the molecular mechanism exploring the action thereof can help to understand the broad-spectrum resistance mechanism of the powdery mildew.
Owner:NANJING AGRICULTURAL UNIVERSITY
Haynaldia villosa CERK1-V gene, and encoded protein and application thereof
The present invention discloses a haynaldia villosa CERK1-V gene, and an encoded protein and an application thereof. A cDNA sequence of the CERK1-V is shown in SEQ ID NO.1 and an amino acid sequence encoded by the gene is shown in SEQ ID NO.2. A single cell transient expression technology transforms a CERK1-V gene overexpression vector pBI220-CERK1-V into a susceptible wheat variety Yangmai 158 and results show that transient expression of the CERK1-V can reduce haustorium indexes of the Yangmai 158. Expression amount of the CERK1-V in overexpression CERK1-V transgenosis plants is 2-12 times of the expression amount of the Yangmai 158 and shows middle and high resistance levels of wheat powdery mildew. Thus, the CERK1-V is expected to be used for gene engineering breeding and introductionof the overexpression vector pBI220-CERK1-V into the susceptible powdery mildew wheat variety is expected to improve the powdery mildew resistance of the wheat.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for transient expression of wheat leaf single cell based on efficient gene gun conversion and application of method
InactiveCN102978230AOptimize bombardment parametersEfficient conversion systemMicrobiological testing/measurementVector-based foreign material introductionDiseaseCuticle
The invention discloses a method for transient expression of a wheat leaf single cell based on efficient gene gun conversion and application of the method. The method for transient expression of the wheat leaf single cell based on the efficient gene gun conversion comprises the following steps of: performing gene gun conversion by using a green fluorescent protein gene as a report gene and a detached leaf of a first wheat leaf as a conversion receptor; immobilizing the wheat leaf by a solid fresh-keeping culture medium, wherein tungsten powder with the diameter of 1 micron is used for gene gun conversion, the using amount is 300ug / gun and the using amount of plasmid is 750ng / gun; and an attacking distance is measured based on 1350psi of cracking membrane helium pressure, a distance between the cracking film and a receptor film is 6 mm, and a distance between the receptor and a stopping net is 6 cm. By the method, haynaldia villosa SGT1 genes are expressed excessively in the detached leaf epidermis cells of the Yangmai 158 of the disease-sensing wheat categories; and a result shows that the index of powdery mildew haustorium in interaction cells can be reduced by the gene transient expression and the invasion of powdery mildew and the formation of the haustorium can be inhibited by the gene so as to increase the resistance of the powdery mildew.
Owner:NANJING AGRICULTURAL UNIVERSITY
Haynaldia villosa vacuolar processing enzyme gene VPE3-V and its silencing vector and application thereof
The invention discloses a haynaldia villosa vacuolar processing enzyme gene VPE3-V and its silencing vector and an application thereof. A cDNA sequence of VPE3-V is SEQ ID NO. 1 and its encoded aminoacid sequence is SEQ ID NO. 2. The gene derives from diploid haynaldia villosa (haynaldia villosa VV, 2n=14), and interacts with the powdery mildew resistance-related gene CMPG1-V, and is mainly located in a cell membrane, and in the powdery mildew resistant diploid haynaldia villosa, the gene is down-regulated significantly after being induced by powdery mildew. Through transient expression and transgenic experiments, the gene is transformed to an infected wheat variety Yangmai 158, and the results show that the silencing wheat VPE3 can improve the resistance to powdery mildew of Yangmai 158.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for transient expression of wheat leaf single cell based on efficient gene gun conversion and application of method
InactiveCN102978230BOptimize bombardment parametersEfficient conversion systemMicrobiological testing/measurementVector-based foreign material introductionDiseaseCuticle
The invention discloses a method for transient expression of a wheat leaf single cell based on efficient gene gun conversion and application of the method. The method for transient expression of the wheat leaf single cell based on the efficient gene gun conversion comprises the following steps of: performing gene gun conversion by using a green fluorescent protein gene as a report gene and a detached leaf of a first wheat leaf as a conversion receptor; immobilizing the wheat leaf by a solid fresh-keeping culture medium, wherein tungsten powder with the diameter of 1 micron is used for gene gun conversion, the using amount is 300ug / gun and the using amount of plasmid is 750ng / gun; and an attacking distance is measured based on 1350psi of cracking membrane helium pressure, a distance between the cracking film and a receptor film is 6 mm, and a distance between the receptor and a stopping net is 6 cm. By the method, haynaldia villosa SGT1 genes are expressed excessively in the detached leaf epidermis cells of the Yangmai 158 of the disease-sensing wheat categories; and a result shows that the index of powdery mildew haustorium in interaction cells can be reduced by the gene transient expression and the invasion of powdery mildew and the formation of the haustorium can be inhibited by the gene so as to increase the resistance of the powdery mildew.
Owner:NANJING AGRICULTURAL UNIVERSITY
Specific Molecular Markers of Chromosomes of Trichophyllum 4vs Against Wheat Yellow Mosaic Virus
ActiveCN103233006BStable resistanceImprove use valueMicrobiological testing/measurementDNA/RNA fragmentationTriticeaeHaynaldia villosa
Owner:NANJING AGRICULTURAL UNIVERSITY
A Disulfide Isomerase Gene and Its Application
Owner:NANJING AGRICULTURAL UNIVERSITY
Trichophyllum 6vs chromosome-specific molecular marker 6vs-bh1 and its use
InactiveCN104877996BStrong brightnessWide range of annealing temperaturesMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyMarker-assisted selection
Owner:JIANGSU UNIV
A complete set of molecular markers for detecting the chromosome arms of T. villosa and its application
ActiveCN107502675BAchieving Specific DetectionMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologySpecific detection
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
A kinase gene lerlk1-v and its expression vector and application
ActiveCN108342401BPowdery mildew resistantTransferasesFermentationGenetic engineeringHaynaldia villosa
The invention discloses a kinase gene LeRLK1-V, and an expression vector and application thereof, belonging to the field of genetic engineering. The cDNA sequence of LeRLK1-V is SEQ ID No. 1, and an amino acid sequence coded by LeRLK1-V is SEQ ID No. 2. The gene LeRLK1-V is derived from the 6VS chromosome arm of the Triticum aestivum-Haynaldia villosa translocation line 6VS / 6AL Nannong 9918. LeRLK1-V is enhanced in expression under the induction of powdery mildew in the powdery-mildew-resistant wheat variety Nannong 9918. The gene is used for transforming the susceptible wheat variety Yangmai158 by transient expression, and results show that the overexpression of LeRLK1-V can reduce the haustorium index of Yangmai 158. Therefore, LeRLK1-V is expected to be applied to genetic engineering breeding; and as LeRLK1-V is introduced into wheat varieties susceptible to powdery mildew, the powdery mildew resistance of the wheat varieties is expected to be improved.
Owner:NANJING AGRICULTURAL UNIVERSITY
A kind of molecular marker primer and its use and pcr method of 3v chromosome of tufted wheat
InactiveCN106148334BSpeed up the transfer processImprove utilizationMicrobiological testing/measurementDNA/RNA fragmentationAgricultural sciencePcr method
Owner:SAAS BIOTECH & NUCLEAR TECH RES INST
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