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Specific codominant molecular marker of haynaldia villosa 4VL chromosome as well as primers and application of molecular marker

A molecular marker, chromosomal technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of limiting the mining, positioning and utilization of excellent genes, and the small number of specific molecular markers.

Inactive Publication Date: 2017-11-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, at present, the number of specific molecular markers on each chromosome of A. villosa is relatively small, and they are mainly distributed on the 4VS and 6VS chromosome arms of W. Less distribution, thus limiting the mining, location and utilization of excellent genes on other chromosomes

Method used

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  • Specific codominant molecular marker of haynaldia villosa 4VL chromosome as well as primers and application of molecular marker
  • Specific codominant molecular marker of haynaldia villosa 4VL chromosome as well as primers and application of molecular marker
  • Specific codominant molecular marker of haynaldia villosa 4VL chromosome as well as primers and application of molecular marker

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Embodiment 1

[0028] 1. Design of molecular marker primers for specific tracking of the 4VL chromosome of T. villosa

[0029] In the research of developing and screening the specific molecular markers for the 4VL chromosome of A. villosa, the molecular marker CINAU321 can specifically track the 4VL chromosome of A. image 3). This pair of labeled primers is based on the EST sequence (BE637255) on the fourth homology group of wheat (see reference Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD et al (2004) A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat.Genetics168:701-712), designed and screened using the online primer design software Primer3V0.4.0 (http: / / frodo.wi.mit.edu / primer3 / ).

[0030] The primers labeled CINAU321 are CINAU321F: 5'-TGTTGATGACATTCTGCACT-3' (SEQ ID NO.1) and CINAU321R: 5'-GCTGACGTATTTGTCTTTCC-3' (SEQ ID NO.2); ( figure 2 )

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Abstract

The invention discloses a specific codominant molecular marker of a haynaldia villosa 4VL chromosome as well as primers and an application of the molecular marker and belongs to the biotechnology field. The invention firstly discloses a molecular marker CINAU321 capable of tracking the haynaldia villosa 4VL chromosome. In a material containing the haynaldia villosa 4VL chromosome, marker primers CINAU321F / CINAU321R can amplify about 1,150 bp products, and the pair of primers can specifically track the haynaldia villosa 4VL chromosome. A disclosed haynaldia villosa T4DS.4VL homozygous compensatory translocation line can provide germplasm resources for mining of excellent genes of the haynaldia villosa 4VL chromosome; the specific molecular marker capable of specifically tracking the haynaldia villosa 4VL chromosome can be used for molecular marker assisted selective breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a co-dominant molecular marker specific to the 4VL chromosome of T. villosa and its primer and application. Background technique [0002] The increasingly narrow genetic basis of cultivars has become the main bottleneck for breakthroughs in wheat breeding (Li Zhensheng, 2010). The close relatives of wheat are rich in genetic resources such as resistance to biotic and abiotic stresses, high yield, and high-quality factors. The introduction of good genes from close relatives into common wheat through distant hybridization is a breakthrough in broadening the genetic basis of wheat and promoting wheat breeding. An effective way to progress (Ji Wanquan et al., 2001; Zhang Zengyan et al., 2004; Wang Shumin et al., 2011). [0003] Haynaldia villosa (genome VV), native to the Mediterranean coast, is a diploid relative of cultivated wheat. It is highly resistant or immune to almost all physiol...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 王海燕宋晶晶赵仁慧贾琪肖进袁春霞王秀娥
Owner NANJING AGRICULTURAL UNIVERSITY
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