Molecular marker closely linked to the wheat stalk rot resistance QTL and application thereof

A technology of stem rot and molecular markers, applied in the fields of wheat breeding and molecular biology, can solve the time-consuming and labor-intensive problems of transferring disease-resistant QTLs, improve breeding efficiency, stabilize PCR amplification, and reduce waste of manpower and material resources Effect

Active Publication Date: 2019-11-29
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
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Problems solved by technology

In addition, the QTL markers that have been reported so far are obtained from foreign disease-resistant germplasm, and the QTL markers used in domestic wheat breeding have not been reported yet.
Most of the reported wheat varieties containing stem rot resistance QTL are Australian wheat varieties. Due to the differences in climatic conditions and other wheat diseases in the wheat ecological zone between Australia and my country, and the fact that most of the disease resistance QTLs are minor QTLs, the wheat varieties in my country are mostly It is time-consuming and labor-intensive to transfer QTLs for disease resistance in varieties. Therefore, mining the QTLs for stem rot resistance in Chinese wheat varieties is of great significance for breeding disease-resistant varieties suitable for my country's wheat ecological zones.

Method used

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  • Molecular marker closely linked to the wheat stalk rot resistance QTL and application thereof
  • Molecular marker closely linked to the wheat stalk rot resistance QTL and application thereof
  • Molecular marker closely linked to the wheat stalk rot resistance QTL and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] F 2 plant, F 2 The offspring of the plant adopt the method of single grain propagation, and finally obtain F 6 Recombinant inbred line populations; use SSR and SNP and other markers to analyze the genotype of the obtained recombinant inbred line populations, and construct genetic linkage maps using mapping software such as JoinMap; Disease resistance identification; comprehensive genetic linkage map and stem rot resistance identification were analyzed by MapQTL analysis software to locate disease resistance QTL and obtain molecular markers closely related to disease resistance QTL. Xgwm37-140 shown in the present invention is a molecular marker closely linked with the 7D chromosome resistance QTL of Yangmai 158. This tag can be obtained by:

[0027] DNA from leaves of CI12633 and Yangmai 158 was extracted by CTAB method (Proc Natl Acad Sci USA, 1984, 81(24):8014-8018).

[0028] PCR amplification was performed using the following reaction system and operating procedu...

Embodiment 2

[0035] 1. Predict the strain resistance of CR-A-1~CR-A-40 and CR-B-1~CR-B-24

[0036] In the examples, the strains defined as CR-A-1~CR-A-40 and CR-B-1~CR-B-24 are based on CI12633 as the male parent and Yangmai 158 as the female parent, crossed and multiplied to f 8 Generation of high-generation strains.

[0037] (1) Extract DNA from leaves of CR-A-1-CR-A-40 and CR-B-1-CR-B-24 lines by CTAB method.

[0038] (2) Using the wheat DNA obtained in step (1) as a template and using SEQ NO:1 and SEQ NO:2 as primers to perform PCR amplification.

[0039] PCR reaction system: total volume 20μl, including 10×buffer 2μl, 1.5mM MgCl 2 , 0.2mM dNTPs, 0.25μM each of SEQ NO:1 and SEQ NO:2, 50ng of template DNA.

[0040] PCR operation program: pre-denaturation at 94°C for 5 minutes; then denaturation at 94°C for 30 sec, annealing at 55°C for 30 sec, and extension at 72°C for 30 sec, a total of 40 cycles; finally, extension at 72°C for 5 min.

[0041] (3) After the amplified product is se...

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Abstract

The invention discloses a molecular marker Xgwm37-140 closely linked to the wheat stalk rot resistance QTL and application thereof. The molecular marker is a 140bp DNA fragment obtained by using wheatDNA as a template, performing PCR amplification through PCR primers with nucleotide sequence as shown in SEQ No.1 and SEQ No.2 and separating through 12% polyacrylamide gel electrophoresis. Accordingto the molecular marker Xgwm37-140, the wheat stalk rot resistance can be predicted and screened by detecting the molecular marker indoors, so as to eliminate infected plants and improve the breedingefficiency.

Description

technical field [0001] The invention relates to the fields of wheat breeding and molecular biology, in particular to a molecular marker closely linked with wheat stalk rot resistance QTL and its application. Background technique [0002] Wheat crown rot, also known as Fusarium crown rot, is an important worldwide wheat disease that occurs in arid and semi-arid regions. Occurs in the southern Pacific coast, Canada, Italy, the Middle East, North Africa and China (Molecular Plant Pathology, 2018, 19(7): 1547-1562). The disease is mainly caused by Fusarium graminearum in Australia (Plant Breeding, 2015,134:365-372), and in China by Fusarium graminearum and Fusarium asiaticum. However, F. graminearum has been on the rise in recent years (Journal of Wheat Crops, 2016, 36(11):1547-1552). The pathogen begins to infect the base of the wheat stem, and the leaf sheaths and stems at the base of the stem become brown at the seedling stage, sometimes causing browning and rot at the root...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 周淼平张鹏陈达姚金保杨学明马鸿翔
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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