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Molecular markers linked with disease resistance gene Pm21 of wheat and application of molecular markers in breeding

A disease-resistant gene and molecular marker technology, applied in the field of molecular genetics and breeding, can solve problems such as easy loss of disease resistance, and achieve the effect of convenient and rapid identification and improved efficiency

Active Publication Date: 2018-02-16
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, 85 powdery mildew resistance genes have been excavated from 58 loci in wheat and its related species, but most of these genes are race-specific resistance and are prone to lose their disease resistance (Wiersma AT, Pulman JA, Brown LK, Cowger C, Olson EL. Identification of Pm58 from Aegilops tauschii. Theor ApplGenet, 2017, 130:1123-1133)

Method used

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  • Molecular markers linked with disease resistance gene Pm21 of wheat and application of molecular markers in breeding
  • Molecular markers linked with disease resistance gene Pm21 of wheat and application of molecular markers in breeding
  • Molecular markers linked with disease resistance gene Pm21 of wheat and application of molecular markers in breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Creation of the wheat-T. villosa minimal fragment translocation line NAU427 carrying the Pm21 gene

[0027] (1) In Jiangsu Academy of Agricultural Sciences, 1600 doses of 60The ears of T6VS·6AL whole-arm translocation line 92R137 were irradiated with COγ-rays 2-3 days before flowering. The irradiated ears were emasculated on the same day, and pollinated with normal Chinese spring pollen 2-3 days later to obtain structural variation Body M1 (Chen PD, You CF, Hu Y, Chen SW, Zhou B, Cao AZ, Wang X (2013) Radiation-induced translocations with reduced Haynaldia villosa chromatin at the Pm21 locus for powdery mildew resistance in wheat. Mol Breed 31:477- 484:).

[0028] (2) The M1 seeds are planted in the field, and the powdery mildew inoculation identification is carried out at the seedling and adult plant stages, the plants with high resistance to wheat powdery mildew are screened, and the seeds are harvested at the mature stage.

[0029] (3) The root tips were...

Embodiment 2

[0032] Example 2. Analysis of the disease resistance effect of the wheat-T. villosa minimal fragment translocation line NAU427

[0033] (1) In order to analyze the disease resistance effect of the translocation fragments in the wheat-T. villosa translocation line NAU427, NAU427 was crossed with Shi 4185 and Yangmai 158, popular varieties susceptible to powdery mildew in different ecological zones, to obtain F1, and then The popularized varieties Shi 4185 and Yangmai 158 were used as recurrent parents for multiple backcrosses, and the disease resistance and molecular marker CINAU1704 were identified in each generation. In the backcross BC4 generation, the disease-resistant plants were self-crossed to harvest seeds BC4F1.

[0034] (2) Plant the selfed seeds in the plastic greenhouse of Jiangpu Farm, Nanjing Agricultural University, with a row length of 1.2m and a row spacing of 20cm, and 10 seeds are sown in each row, and the individual plants are identified for powdery mildew re...

Embodiment 3

[0037] Example 3. Development of Molecular Markers Linked to Pm21 in the Wheat-T. villosa Translocation Line NAU427 (1) Development of Molecular Markers Linked to Pm21 in NAU427

[0038] 1.1 Determination of the collinear segment of the introgressed T. villosa translocation segment with Brachypodium and rice

[0039] Both NAU418 and NAU427 contained the Pm21 gene, and the results of in situ hybridization showed that the translocation fragment of NAU418 was larger than that of NAU427. In order to develop molecular markers linked to the Pm21 gene in the NAU427 translocation fragment, the EST sequences of four markers CINAU273, CINAU274, CINAU275, and CINAU15, which amplified 6VS-specific bands in the small fragment translocation line NAU418, were first extracted. Compare these 4 EST sequences with the genome sequences of Brachypodium (http: / / plants.ensembl.org / Brachypodium_distachyon / Info / Index) and rice (http: / / plants.ensembl.org / Oryza_sativa / Info / Index) Comparison, the result...

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Abstract

The invention discloses molecular markers linked with a disease resistance gene Pm21 of wheat and application of the molecular markers in breeding. 25 molecular markers are derived from Triticum aestivum-Haynaldia villosa translocation line NAU427 of the powdery mildew resisting gene Pm21, are tightly linked with the gene Pm21 and can be used for assistant selection of the molecular markers; and selection efficiency and accuracy of disease resisting plants are improved.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and relates to a molecular marker linked with wheat disease resistance gene Pm21 and its application in breeding. Background technique [0002] Wheat is an important global food crop, and 35%-40% of people in the world rely on wheat as a staple food. Wheat powdery mildew is one of the important diseases in the world, which can lead to 13-34% yield loss in a normal disease year, and more than 50% yield loss in a severe disease year. Breeding wheat varieties with high resistance to powdery mildew is the most economical, safe and effective way. In recent years, with the development of molecular marker technology and the construction of high-density molecular genetic maps, molecular marker-assisted breeding has played an increasingly important role in the research of wheat elite traits breeding. Excavating germplasm resources containing powdery mildew resistance genes and developing molecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11C12N5/10
CPCC12N5/04C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 邢莉萍胡平刘佳倩周渭皓曹爱忠陈佩度张瑞奇王秀娥张守忠
Owner NANJING AGRICULTURAL UNIVERSITY
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