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Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker

A chromosome arm and molecular marker technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problems of rapid variation of physiological races.

Active Publication Date: 2016-05-18
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the physiological races of powdery mildew mutate quickly, and many disease-resistant genes are overcome by new races soon after they are used in production.

Method used

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  • Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker
  • Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker
  • Set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and molecular marker

Examples

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Effect test

Embodiment 1

[0085] Example 1. Detection of whether wheat contains tufted wheat chromosome arms

[0086] 1. Preparation of a complete set of reagents for detecting whether wheat contains tufted wheat chromosome arms

[0087] The complete set of reagents for detecting whether wheat contains the chromosome arm of tufted wheat is set of reagent 1 and set of reagent 2, and the set of reagent 1 is composed of A-P, B-P, C-P, D-P and E-P;

[0088] A-P consists of two single-stranded DNAs shown in Sequence 1 and Sequence 2 in the sequence listing;

[0089] B-P consists of two single-stranded DNAs shown in Sequence 3 and Sequence 4 in the Sequence Listing;

[0090] C-P consists of two single-stranded DNAs shown in Sequence 5 and Sequence 6 in the sequence listing;

[0091] D-P consists of two single-stranded DNAs shown in sequence 7 and sequence 8 in the sequence listing;

[0092] E-P consists of two single-stranded DNAs shown in sequence 9 and sequence 10 in the sequence listing;

[0093] Kit ...

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Abstract

The invention discloses a set reagent for detecting whether wheat contains haynaldia villosa 6VS chromosome arms or not and a molecular marker. The set reagent is composed of A-P and at lease one of B-P, C-P, D-P and E-P; the A-P is composed of two single-stranded DNA shown in a sequence 1 and a sequence 2 in a sequence list; the B-P is composed of two single-stranded DNA shown in a sequence 3 and a sequence 4 in the sequence list; the C-P is composed of two single-stranded DNA shown in a sequence 5 and a sequence 6 in the sequence list; the D-P is composed of two single-stranded DNA shown in a sequence 7 and a sequence 8 in the sequence list; the E-P is composed of two single-stranded DNA shown in a sequence 9 and a sequence 10 in the sequence list. The set reagent and a method can be used for screening a wheat cross population and identifying and assistantly breeding a new wheat line or variety with the excellent property (such as the powdery mildew resistance) of haynaldia villosa.

Description

technical field [0001] The invention relates to a complete set of reagents and molecular markers for detecting whether the genetic background of wheat contains the 6VS chromosome arm of tufted wheat in the field of biotechnology. Background technique [0002] Dasypyrumvillosum (L.) P. Candargy (syn. Haynaldiavillosa Schur) is a diploid species of the Triticum genus Dasypyrumvillosum, 2n=2x=14. It originated in the northeastern Mediterranean, from southern France in southern Europe to the Caspian Sea, southwestern Asia, Russia and the Caucasus (DePace et al. 1995; Frederiksen 1991), and is a weedy plant that grows in harsh, arid environments (AgnieszkaGradzielewska, 1991). 2006). It is named because of the clumps of hairs on the glume ridges and the top of the lemma. [0003] The tufted wheat contains many biotic and abiotic stress resistance genes and high-quality genes, and is an excellent gene source for improving wheat. Sears, Lukaszewski and Liu Dajun successively tra...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 林志珊刘畅叶兴国王轲杜丽璞李仕金陈孝
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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