A complete set of molecular markers for detecting the chromosome arms of T. villosa and its application

Abstract

The invention discloses a complete set of molecular markers for detecting the chromosome arms of Haynaldia villosa and application thereof. The invention provides the complete set of molecular markers for detecting the chromosome arms of Haynaldia villosa, and the complete set of molecular markers are composed of all the or a part of primer pairs (14 primer pairs as shown in sequence 1 to 28 in a sequence table) used for detecting 2V, 3V, 4V, 6V and 7V chromosome arms of Haynaldia villosa. According to the invention, RNA-seq technology is employed for acquiring the transcriptome sequences of Haynaldia villosa; primers are designed on the basis of the transcriptome sequences; and specific markers capable of accurately identifying each chromosome of Haynaldia villosa are screened out, and corresponding amplification primers are designed. Experimental results show that the complete set of primers can realize specific detection of each chromosome of Haynaldia villosa and are successively applied to detection of Haynaldia villosa chromosomes in Triticum durum-Haynaldia villosa amphidiploid and wheat hybrid and backcross generation materials. The complete set of molecular markers in the invention provide a more convenient and effective tool for further research and utilization of Haynaldia villosa.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a set of molecular markers for detecting chromosomal arms of T. villosa and an application thereof. Background technique [0002] Dasypyrum villosum (L.) P.Candargy (syn. Haynaldia villosa Schur) is an annual or perennial diploid plant (2n=2x=14, Sears will The genome is named VV). It originates in the northeastern part of the Mediterranean, from southern France in southern Europe to the Caspian Sea, southwestern Asia, Russia and the Caucasus, and is a weedy plant that grows in harsh, arid environments. It is named because of the clusters of hairs on the glume ridges and the tops of the lemmas. Dust wheat has the characteristics of resistance to stripe rust, leaf rust, stem rust, powdery mildew and wheat shuttle mosaic virus and other major wheat diseases and insect pests. It also has cold resistance, strong tillering ability, luxuriant growth, many small flowers, high protein conten...

AI Technical Summary

Problems solved by technology

Up to now, the research and utilization of diploid Trichotilloides is not enough and needs to be strengthened. For example, there are few reports on the cloning of specific DNA fragments on specific chromosomes and the development of molecular markers.

There have been some reports on the specific markers of T. villosa, but most of them are concentrated on chromosome 6VS. Currently, markers distributed on chromosomes 1V to 7V mainly include SSR, ISSR, and EST-STS markers. These markers are used to identify a large number of structural variant chromosomes of T. villosa , which is far from meeting the needs of a large number of molecular genetics and breeding studies on the transfer of excellent genes from Trichosanthes villosa to wheat

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