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Specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus

A molecular marker, chromosome technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as narrow genetic basis, and achieve the effect of high utilization value and high accuracy

Active Publication Date: 2013-08-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the current breeding work mainly utilizes the major resistance loci on the long arms of wheat chromosome 2D and 5A (Nishio et al., 2010; Zhu et al., 2012), and the genetic basis of wheat resistance to yellow mosaic disease is relatively narrow

Method used

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  • Specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus
  • Specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus
  • Specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus

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Embodiment Construction

[0034] 1. Discovery of wheat yellow mosaic disease resistance gene carried by 4VS

[0035] The identification standard of wheat resistance to yellow mosaic disease is divided into 0-5 grades ( figure 2 ) (References: Zhu X B, Wang H Y, Guo J, Wu Z Z, Cao A Z, Bie T D, Nie M J, You F M, Cheng Z B, Xiao J, Liu Y Y, Cheng S H, Chen P D, Wang X E. Mapping and validation of quantitative trait loci associated with wheat yellow mosaic bymovirus resistance in bread wheat. Theoretical and Applied Genetics, 2012, 124(1): 177-188.): Grade 0 means the plants are asymptomatic; Grade 1 means the plants are not obviously dwarfed, Mildly mosaic, generally no obvious striated necrotic spots, no yellowing or a few yellowing leaves; level 2: plant dwarfing not obvious, mild mosaic, obvious striated necrotic spots, obvious yellowing leaves; 3 Grade 4: Plants are slightly dwarfed, mosaic is obvious, striated necrotic spots account for about 1 / 2 of the leaf area, some leaves are yellowed, a few a...

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Abstract

The invention discloses specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus and belongs to the field of biotechnology. The invention firstly discloses two molecular markers CINAU66 and CINAU295 which are capable of tracking the haynaldia villosa 4VS chromosome. In materials containing the haynaldia villosa 4VS chromosome, marker primers CINAU66F / CINAU66R are capable of amplifying about 950bp (base pair) of product; and marker primers CINAU295F / CINAU295R are capable of amplifying about 380bp of product; and the primers are capable of tracking the haynaldia villosa 4VS chromosome. The haynaldia villosa 4VS chromosome capable of resisting the wheat yellow mosaic virus, disclosed by the invention, is capable of proving new gene resources for the breeding of yellow mosaic virus resisting wheat; and the specific molecular markers capable of specifically tracking the haynaldia villosa 4VS chromosome can be used for molecular marker-assisted selective breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a specific molecular marker of wheat yellow mosaic virus-resistant wheat yellow mosaic virus 4VS chromosome. Background technique [0002] Wheat yellow mosaic (WYMV) is a soil-borne viral disease caused by wheat yellow mosaic bymovirus, and the dormant sporangia of Polymyxa graminis It can survive for more than several years under dry soil conditions. It can be spread through agricultural operations, diseased soil, diseased root residues, etc., or mixed with mediator soil in seeds for long-distance transmission. In fields where wheat yellow mosaic disease occurs, the yield can generally be reduced by 20-30%, and in severe cases it can reach 70%, or even no harvest (Liu et al., 2004; Liu et al., 2005a, 2005b). The area of ​​wheat yellow mosaic disease in my country is more than 10 million mu every year, and it shows a trend of expansion year by year. The threat of epidemic is gradually...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 王海燕赵仁慧陈忠明肖进曹爱忠贾琪袁春霞王秀娥
Owner NANJING AGRICULTURAL UNIVERSITY
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