Gossypium barbadense chromosome segment capable of improving verticillium wilt resistance of gossypium hirsutum and molecular markers

Abstract

The invention discloses a gossypium barbadense chromosome segment capable of remarkably improving verticillium wilt resistance of gossypium hirsutum and molecular markers of the gossypium barbadense chromosome segment. The gossypium barbadense chromosome segment D4-1 is derived from gossypium barbadense H7124, is located in NO. D4 chromosome of cotton chromosome sets, and is identified through 11 pairs of SSR (simple sequence repeat) markers (including NAU3392, NAU6992, NAU6993, NAU3791, cgr6409, JESPR220, NAU5294, NAU7290, ZHX1, ZHX6 and ZHX29). DNA of the gossypium barbadense H7124 is taken as a template, the 11 pairs of SSR markers are adopted for amplification simultaneously, and a chromosome segment capable of simultaneously containing 11 SSR marker specific bands is the chromosome segment D4-1 of the gossypium barbadense H7124. A disease index of a gossypium hirsutum introgression line containing the chromosome segment is obviously lower than that of a gossypium hirsutum receptor control variety Sumian 8. The gossypium barbadense chromosome segment and the molecular markers thereof are applied to the verticillium wilt resistant molecular breeding of cotton, the verticillium wilt resistance of the cotton can be greatly improved, and the breeding efficiency of the cotton is improved.

Description

technical field [0001] The invention belongs to the technical field of crop genetics and breeding, and relates to a sea-island cotton chromosome fragment and a molecular marker capable of significantly improving the resistance of upland cotton to verticillium wilt. Background technique [0002] Cotton is the main economic crop in China, and cotton production has always played a decisive role in my country's national economy. There are many diseases of cotton, especially Verticillium wilt, which cause huge losses to cotton production. Cotton verticillium wilt was discovered and reported by Carpenter in Virginia, USA in 1914. It is a fungal disease caused by Verticillium dahliae. [0003] Cotton verticillium wilt in my country was introduced into my country in 1935 due to the introduction of the 4B cotton seed from the United States. Later, with the breeding and transportation of cotton seeds, cotton verticillium wilt gradually spread in major cotton-producing areas in my cou...

AI Technical Summary

Problems solved by technology

In 2002 and 2003, cotton verticillium wilt occurred again and again in the northern cotton area, causing heavy losses to co

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