Improved and simplified plant chromosome fluorescence in-situ hybridization method

Abstract

The present invention provides an improved and simplified plant chromosome fluorescence in-situ hybridization method, wherein the method does not include a long-time dewatering process of a specimen slide before hybridization and multiple rinsing processes of the specimen slide after hybridization, such that the method is the technology for carrying out rapid in-situ hybridization on the intranuclear chromosome in a biological cell sample. The method mainly comprises: 1) carrying out variable temperature germination, 8-hydroxy quinoline pretreatment, and fixation of the strong cell division tissue in the plant root tip meristem region with a Carnoy fixation liquid to obtain a metakinesis phase specimen with characteristics of clear image and good chromosome dispersion; 2) after mixing fluorescent probes having different labels, carrying out one time denaturation, and carrying out low temperature storage so as to achieve long term repeated use; 3) carrying out denaturation on the sample slide with a NaOH alcohol solution; and 4) during a hybridization reaction process, simplifying the multi-step dewatering, rinsing and other operation steps so as to substantially simplify the whole complex hybridization process. According to the present invention, the method is particularly suitable for the rapid large-scale detection and analysis of the exogenous chromosomes fragment and the chromosome fragment in the plant distant hybridization and close hybridization species, and the morphology and structure authenticity of the specimen chromosome is well maintained.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows researchers to study cells that form tissues called callus when they divide into different parts like leaves, stems, bark, flowers, seeds, cerebral tubules, heartbanks, muscle fibers, nerves, blood vessels, cardiac valves, liver slices, brain nuclei, spines, neurons, and other structures involved in biological systems such as photosynthesis, fertilizing nitrogen nutrients, regulating gene expression levels, controllably altered genesis rates, and detecting specific types of organisms within these organs. It also includes various techniques related to enzyme activation, nucleic acids extraction from samples containing target substances, separation/isolation methods, and purification protocols. Overall, this innovative technical results improve understanding of how stem cells grow and develop throughout their life cycle while minimizing environmental impact on them.

Problems solved by technology

The technical problem addressed in this patented patent relates to improving the performance and reliability of traditional fluorescein indiscret stainless steel nuclear magnetic resonance technique that uses specific binding pairs called histidyl protein binder(PHB), nucleoside triphosphatide (NTP). These binders have unique properties making them ideal tools for studying biological processes like different types of cells including nuclei, cytoplasm, mitochondria, chloroplasts, and polysaccharides. They provide accurate results even when there're many thousands of samples being analyzed simultaneously without overlapping background signals from other sources.

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