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31 results about "Second polar body" patented technology
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Polar body. polar body n. Either of two small cells produced during the first and second meiotic divisions in the development of an oocyte, containing little cytoplasm and eventually degenerating.
Selection of embryo of test tube baby through sequencing by single cell genome of polar body or embryo
Disclosed is a method for using a first polar body and a second polar body as well as a single-cell embryo to carry out whole-genome non-exponential amplification and high-throughput genome sequencing, so as to perform preimplantation genetic diagnosis for genetic disease and testing for pathogenic genes causing repeated miscarriages. The method of the present invention comprises the following steps: (1) obtaining oocytes and embryos, and carrying out separation and genome amplification of first and second polar bodies and single-cell embryos; (2) establishing a genome sequencing library and sequencing, and carrying out bioinformatic analysis of the genome to obtain a gene spectrum and information concerning the number of copies of chromosomes and fragments thereof; (3) determining the chromosome ploidy of the polar bodies and embryos as well as information concerning defects, replication and point mutation in the chromosome fragments; (4) selecting normal or suitable embryos for implantation.
Owner:HARVARD UNIV +2
Human parthenogenetic embryo stem cell line with two active X chromosomes and derivatives thereof
The invention relates to a human parthenogenetic embryo stem cell line with two active X chromosomes and derivatives thereof. A karyotype of the human parthenogenetic embryo stem cell line is 46, XX before 10 generations; the karyotype of the cell line becomes a chimera from the 20th generation; all cells of the cell line lose an active X chromosome at the 35th generation; and in the inactivation detection of the X chromosome, only the state of the active X chromosome is maintained all along, but the presence of the inactivated X chromosome is not discovered. The parthenogenetic activation can be achieved by adopting an artificial activation condition causing a second polar body to be discharged; therefore, the human parthenogenetic embryo stem cell line and the derivatives thereof can be obtained. The cell line and the derivatives thereof have important significance on researching the characteristics of the cell on genetics and epigenetics and further applying the cell to clinically treating certain diseases; and a cell source for cell replacement therapy can be obtained by transforming genes of the cell or modifying the genes on the epigenetics.
Owner:广州医学院
Production method of triploid yellow catfish
InactiveCN101305698AInhibition release timeHigh induction rateClimate change adaptationPisciculture and aquariaRelease timeArtificial insemination
The invention discloses a production method of triploid pseudobagrus fulvidraco, which belongs to the fish culture technology field. Artificial insemination is performed to the pseudobagrus fulvidraco female parent and male parent which are obtained through artificial induced spawning, after 10 to 15 minutes, triploid pseudobagrus fulvidraco is obtained through induction, and the duration time for induction is 10 to 20 minutes. After the triploid pseudobagrus fulvidraco is obtained through induction, the multiple of the chromosome is measured through the technologies, such as the karyotype analysis of chromosome, the flow cytometer, etc., and the triploid pseudobagrus fulvidraco is identified and sieved for culture. The method restrains the releasing time of the second polar body through time control on the artificial insemination, the induction through the physical or chemical method, and control on the induction duration time, and thereby the inductivity and the survival rate of the triploid pseudobagrus fulvidraco are greatly increased, and according to the experiment statistics, the artificial inductivity of the achieved triploid pseudobagrus fulvidraco is more than 50 percent, and the survival rate is more than 80 percent.
Owner:SHAOXING UNIVERSITY +2
Method for culturing Crassostrea angulata tetraploid
The invention provides a method for culturing a Crassostrea angulata tetraploid. The method comprises the steps of firstly culturing a pacific oyster tetraploid, hybridizing the pacific oyster tetraploid with Crassostrea angulata so as to culture a hybridized triploid, fertilizing the hybridized Crassostrea angulata triploid with a Crassostrea angulata diploid sperm, carrying out anesthetization by virtue of magnesium chloride mixed liquid to inhibit the release of a second polar body so as to generate the tetraploid, wherein an inductive agent is safe and toxic-free. According to the method,the long induction process of the Crassostrea angulata triploid is solved, the survivable and stable tetraploid Crassostrea angulata can be cultured by virtue of the technical routes of hybridization,high permeation, anesthetization, molecular marking and the like, and the foundation is laid for the large-scale seedling growing of triploid Crassostrea angulata.
Owner:OCEAN UNIV OF CHINA
Gynogenesis method of grass carp and application thereof
ActiveCN101669449AImprove hatchabilityHigh activityClimate change adaptationPisciculture and aquariaOosporeShock treatments
The invention discloses a gynogenesis method of grass carp and application thereof. The method comprises the following steps: taking the diploid sperms of a properly inactivated allotetraploid cruciancarp as a stimulus to stimulate mature eggs of the grass carp, after activating for 2-3 min or fertilizing for 20-22 min, carrying out the water-cooling shock treatment for 10-12 min at 4-6 DEG C, and hatching and controlling the temperature by to obtain the gynogenesis grass carp restraining the discharge of a second polar body or the first cleavage. The method greatly improves the hatching rateand the survival rate of the gynogenesis diploid grass carp, simplifies the preparation process, and can prepare a gynogenesis grass carp colony with good characters and stable heredity.
Owner:HUNAN NORMAL UNIVERSITY
Preparation method of young mollusks of Hongkong tetraploid oysters
ActiveCN107494358ABreaking the monopolyClimate change adaptationPisciculture and aquariaMud oysterMatus ovatus
The invention discloses a preparation method of young mollusks of Hongkong tetraploid oysters. The method comprises the following technical steps of selectively choosing triploid eggs, carefully choosing diploid sperms, inhibiting second polar bodies of oosperms, obtaining tetraploid young mollusks, and raising tetraploid progenies. The feature of normal reduction mitosis of some triploid eggs is adopted so as to create a raising method for preparing tetraploid oysters by inhibiting second polar bodies of oosperms (triploid eggs +diploid sperms). The method differs from a method for obtaining tetraploid oysters by inhibiting first polar bodies of oosperms. The preparation method makes a great difference in a traditional preparation mode and helps break the monopolies of the US, France, Korea and other foreign countries on raisins technology of tetraploid oysters. It is the first time to obtain livable young mollusks of Hongkong tetraploid oysters. The method makes the biological method of producing 100% Hongkong triploid oysters feasible, promote the steady development of the industry of Hongkong oysters in the coastal region of southern China and offers the possibility of achieving improved varieties of the industry. The preparation method of young mollusks of Hongkong tetraploid oysters is being advantaged by being implementable, reusable, and applicable.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Abalone triploid pharmaceutical induction method
InactiveCN1733908AStable magnificationLow embryonic malformation rateMutant preparationTissue cultureHaliotis discusEmbryo
The invention discloses a method for inducing triploid of Haliotis discus Hannai through medicament, which comprises the following steps, preparing 6-dimethoxy aminopurine solution, obtaining abalone sperms and ova through the conventional method, obtaining oosperm through artificial insemination, placing oosperm of seed abalone into sea water, finally inhibiting the second polar body of the Haliotis discus Hannai with 6-dimethoxy aminopurine solution.
Owner:DALIAN FISHERIES UNIVERSITY
A cultivation method for carassius auratus gynogenesis fries
ActiveCN104904638APure germplasmFast growthClimate change adaptationPisciculture and aquariaGenetic MaterialsMicrosatellite
The invention provides a cultivation method for carassius auratus gynogenesis fries. The method comprises the steps of selecting sexually mature diploid carassius auratus parents and collecting sperm and ovums; performing ultraviolet irradiation on Hanks liquid-diluted sperms to inactivate genetic materials thereof; performing artificial insemination on the inactivated sperms and the ovums; restraining the discharge of second polar bodies of meiosis by using a cold shock method to double genomes of the ovums and thus to cultivate viable carassius auratus gynogenesis diploid fries. Microsatellite marker identification proves that the genetic materials of all the gynogenesis fries are from the female parent; morphological measurement indicates part of the gynogenesis fries has obvious early growth dominance. The method has the advantages of simple operation, high efficiency, stability and high practicality.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Method for inducing gynogenesis of Siberian sturgeon by using amur sturgeon sperm
InactiveCN101569293AIncrease the proportion of female individualsImprove farming efficiencyClimate change adaptationPisciculture and aquariaStart timeZoology
The invention relates to a method for inducing gynogenesis of a Siberian sturgeon by using an amur sturgeon sperm, which comprises the following steps: (1) inactivating a genetic material of the amur sturgeon sperm; (2) acquiring Siberian sturgeon unfertilized ova and performing insemination with an inactivated sperm of an amur sturgeon; (3) inducing the gynogenesis of the Siberian sturgeon through heat shock: inducing the gynogenesis of the Siberian sturgeon by adopting a method for inhibiting a second polar body from being discharged through the heat shock , wherein the starting time of the heat shock is 0.3 tau0 (18 minutes at a temperature of below 16 DEG C) after fecundation, the duration of the heat shock is 2 minutes, and the temperature of the heat shock is 37 DEG C; and (4) cultivating a gynogenesis induced egg. A super-female fish of the Siberian sturgeon cultivated by the technique of the method can produce gynoecy fish fries by mating with a normally cultivated milter, thus the proportion of female individuals in cultured Siberian sturgeon population is improved, and the cultivation benefit is improved.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Method for inducing ocean seashell polyploidy by using sodium chloride
InactiveCN101658149AInhibition releaseEasy to operateClimate change adaptationPisciculture and aquariaPolar bodyMicroscopic exam
The invention discloses a method for inducing ocean seashell polyploidy by using sodium chloride. The method is characterized by comprising the following steps: selecting the seashell to grow the shell to respectively obtain sperms and ovum; carrying out artificial insemination; continuously observing the developing condition of zygotes through microscopic examination to determine the number of the water body for culturing the zygotes; adding sodium chloride into the culture sea water containing the zygotes according to a proportion that 20-40 go of sodium chloride is added in 1000ml of culture seat water when 40-50 percent of zygotes are observed to release a first polar body or a first zygote is observed to occur the first polar body, so as to prevent the release of a second polar body or the first polar body of the zygotes; evenly stirring and treating for 10-15 min; and transferring the zygotes to normal sea water to incubate . The ocean seashell comprises Pacific oyster, chlamys farreri, Argopecten irradians, Crassostrea rivularis, clam, Scapharca broughtonii and the like. The seashell zygotes are treated by using the sodium chloride and the initiating agent so as to inhibit the release of the polar body and produce the shell polyploidy with no toxicity, efficiency, simple operation and extremely low cost.
Owner:山东安源种业科技有限公司
Method for producing loach tetraploid
InactiveCN106376501APlay application valueProven reliabilityClimate change adaptationPisciculture and aquariaRed blood cellGermplasm
The invention discloses a method for producing a loach tetraploid. The male parent fish is a natural tetraploid loach, the female parent fish is a diploid loach, and dry fertilization is adopted; and after the fertilization, the zygote is treated for 50-60min in water at 0 DEG C and then normally hatched in normal-temperature aerated water. In the invention, two loaches different in chromosome number are adopted, namely the diploid loach (2n=50) and the tetraploid loach (4n=100); the release of a second polar body is inhibited through cold shock treatment, and the tetraploid is induced; and the chromosome number, DNA relative content, red blood cell nucleus volume, chromosome number, micro-satellite and the like of the generated offspring are analyzed relatively systematically, so that the authenticity and reliability of tetraploid loach induction are proven, and the method has application values to the protection and utilization of loach genetic resources and further aquaculture of the loaches.
Owner:DALIAN OCEAN UNIV
Inducing method of Nibea albiflora gynogenesis diploid
InactiveCN103430872AImprove and accelerate homozygous efficiencyOvercoming the Effects of Haploid SyndromeClimate change adaptationPisciculture and aquariaSemenMatus ovatus
The invention discloses an inducing method of a Nibea albiflora gynogenesis diploid. The technical scheme of the invention is that the inducing method comprises the steps of genetic inactivation of sperms of Nibea albiflora and diploidzation of ova of the Nibea albiflora; the inducing method is mainly characterized by firstly enabling the sperms of the Nibea albiflora to be subjected to the genetic inactivation by using ultraviolet irradiation, then activating the sperms and the ova by using seawater after the sperms are mixed with the ova of the Nibea albiflora, starting gynogenesis of the ova of the Nibea albiflora, restraining the discharge of a second polar body by using cold shock, doubling genomes of the ova, and inducing the Nibea albiflora gynogenesis diploid. The inducing method disclosed by the invention has the advantages that the operation is simple, the needed cost is lower, the practicability is stronger, and the like.
Owner:JIMEI UNIV
Polyploid breeding method for inducing heterogenous tripoid scallop by physical means
InactiveCN1769441AGrow fastHigh sterilityMutant preparationElectrical/wave energy microorganism treatmentHeterologousOvulation times
The invention relates to a physical method of polyploidy-breeding by introducing allotriploid scallops, put female scallops in filter seawater at the temperature of 16íµ after being dried in shade for 40 minutes to stimulate ovulation; put male scallops in filter seawater at the temperature of 16íµ after being dried in shade for 1 hour to stimulate sperm-drain, and then put said ovums and sperms produced in filter seawater at the temperature of 20íµ for fertilization following the mixing rate of 1í†5. When 30% of ovums have been drained out of second polar bodies after fertilization, force pressure of 20-30 MP for 20-10 minutes, hatch fertilized ovums according to the conventional method and get triploid scallops. The scallops cultured in said invention are characterized on fast,growth, high infertility and fresh texture, could make up for the shortages of low production and induced texture during the multiplication periods caused by diseases and high-density culturing in recent years.
Owner:OCEAN UNIV OF CHINA
Gynogenesis method of grass carp and application thereof
ActiveCN101669449BImprove hatchabilityHigh activityClimate change adaptationPisciculture and aquariaShock treatmentsObserved Survival
The invention discloses a gynogenesis method of grass carp and application thereof. The method comprises the following steps: taking the diploid sperms of a properly inactivated allotetraploid crucian carp as a stimulus to stimulate mature eggs of the grass carp, after activating for 2-3 min or fertilizing for 20-22 min, carrying out the water-cooling shock treatment for 10-12 min at 4-6 DEG C, and hatching and controlling the temperature by to obtain the gynogenesis grass carp restraining the discharge of a second polar body or the first cleavage. The method greatly improves the hatching rate and the survival rate of the gynogenesis diploid grass carp, simplifies the preparation process, and can prepare a gynogenesis grass carp colony with good characters and stable heredity.
Owner:HUNAN NORMAL UNIVERSITY
Method for obtaining takifugu rubripes androgenesis haploids
InactiveCN106259132AFix damageIncrease development rateClimate change adaptationPisciculture and aquariaIce waterOospore
The invention discloses a method for obtaining takifugu rubripes androgenesis haploids. The steps of the method are as follows: (a) the spawning of male takifugu rubripes and female takifugu rubripes with well-developed gonads is induced, the sperms of the male takifugu rubripes and the ova of the female takifugu rubripes are respectively taken, and the sperms and the ova are mixed to form fertilized ova; (b) the androgenesis of the takifugu rubripes is induced by cold shock. The method adopts the sperms of the male takifugu rubripes and the ova of the female takifugu rubripes to form the fertilized ova; cold shock is used for processing (30 to 60 minutes of immersion in ice water of 2 DEG C to 4 DEG C 5 to 8 minutes after fertilization), the egg nuclei and second polar bodies of the takifugu rubripes are induced to release together, and thereby fertilized ova which only have sperm nuclei are formed. Because the genetic inactivation of ova is not required, a complex operation procedure is avoided, the method is cheap and easy to operate, the problem that conventional physiochemical induction can injure fertilized ova is solved, the androgenesis rate is increased, and the method is suitable for use in mass production.
Owner:DALIAN OCEAN UNIV +1
A kind of preparation method of Hong Kong oyster tetraploid larvae
ActiveCN107494358BBreaking the monopolyHave applicableClimate change adaptationPisciculture and aquariaOysterMitosis
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Method for inducing seashells to generate polyploid
InactiveCN1759814AInhibition releaseEasy to operateAnimal reproductionClimate change adaptationSeawaterSecond polar body
A method for inducing the polyploid of marine shellfish includes such steps as measuring the salinity of culturing seawater, taking the sperms and ova of shellfish, artificial insemination, adding fresh water to seawater until its salinity is 4-16 when 40-50% of fertilized ova is releasing the first polar bodies, stirring for 10-15 min for suppressing the release of the second polar bodies, and incubating the fertilized ova in normal seawater.
Owner:OCEAN UNIV OF CHINA
Continuous batch induction method for salmo salar triploids
ActiveCN111194705AEasy to use in productionAs soon as possible to determine the induction effectClimate change adaptationPisciculture and aquariaAnimal scienceMedicine
The invention relates to chromosome operation technology, and particularly relates to a continuous batch induction method for salmo salar triploids. The method includes the following steps: collectingsalmo salar sperm eggs to carry out artificial insemination, and dividing fertilized eggs into 2-3 groups; and carrying out a hydrostatic pressure induction treatment on each group in a successive and continuous mode to inhibit emissions of second polar bodies, thereby obtaining the salmo salar triploids. According to the hydrostatic pressure induction treatment, 300-400 DEG C x minute (temperature x time) after insemination, the fertilized eggs in Group 1 is subjected to the hydrostatic pressure induction treatment to obtain the triploid salmo salar; after the induction, the fertilized eggsin the Group 1 are taken out, and then the fertilized eggs in Group 2 are immediately performed with the hydrostatic pressure induction according to the above-mentioned process, thus the same batch offertilized eggs can be induced and treated continuously. According to the invention, the method can be used for continuous and efficient induction to obtain 100% salmo salar triploids.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Method for identifying SNP genotype-based monozygotic twins, fraternal twins and second polar body participated fertilized twins
PendingCN111534605AAccurate classificationHigh sensitivityMicrobiological testing/measurementMedicineGenetics
The invention relates to a method for identifying SNP genotype-based monozygotic twins, fraternal twins and second polar body participated fertilized twins. The method comprises the following steps: analyzing the SNP genotype similarity level of father being homozygote and mother being heterozygote in twins, the twins are determined to be fraternal twins if the similarity level is about 50 percent, otherwise, the twins are determined to be non-fraternal twins if the similarity level is more than 90 percent. If the twins are determined to be non-fraternal twins, further analysis is required: the SNP genotype is completely the same in the twins, the twins are determined to be monozygotic twins; and otherwise, if the similarity level x is more than 90 percent and less than 100 percent, the twins are determined to be second polar body participated fertilized twins. The novel and smart identification method is provided for the first time, the blank for identifying monozygotic, fraternal andsecond polar body participated fertilized twins in medical and expert testimony, has high sensitivity and high accuracy, is easy in sample acquisition, simple in operation and high in analyzing efficiency, and is widely applicable to the fields of expert testimony / medical identification and the like.
Owner:THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
A method for breeding Portuguese oyster tetraploids
The invention provides a method for culturing a Crassostrea angulata tetraploid. The method comprises the steps of firstly culturing a pacific oyster tetraploid, hybridizing the pacific oyster tetraploid with Crassostrea angulata so as to culture a hybridized triploid, fertilizing the hybridized Crassostrea angulata triploid with a Crassostrea angulata diploid sperm, carrying out anesthetization by virtue of magnesium chloride mixed liquid to inhibit the release of a second polar body so as to generate the tetraploid, wherein an inductive agent is safe and toxic-free. According to the method,the long induction process of the Crassostrea angulata triploid is solved, the survivable and stable tetraploid Crassostrea angulata can be cultured by virtue of the technical routes of hybridization,high permeation, anesthetization, molecular marking and the like, and the foundation is laid for the large-scale seedling growing of triploid Crassostrea angulata.
Owner:OCEAN UNIV OF CHINA
Culter alburnus gynogenesis inducing method
ActiveCN103563805BEasy to operateImprove hatchabilityClimate change adaptationPisciculture and aquariaZooidAnimal science
The invention discloses a method for inducing the gynogenetic development of Cichlid carp, which comprises the following steps: (1) collecting carp semen; (2) carrying out ultraviolet genetic inactivation treatment on carp semen; Carp semen after ultraviolet genetic inactivation treatment was used for dry insemination; (4) Fertilized eggs were treated with cold shock and placed in hatching containers for hatching. Normal surviving fry were gynogenetic fish. The present invention adopts the ultraviolet genetic inactivation sperm of common carp to stimulate the eggs of the cymbidium, inhibits the release of the second polar body of the eggs by cold shock, and promotes chromosome doubling, and successfully obtains the gynogenetic individuals of the cymbidium. Since the normal surviving individuals are gynogenetic fish, there is no need for subsequent identification and analysis of chromosomes and genetic markers, which significantly simplifies the identification procedure.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES
Polyploid breeding method for inducing heterogenous tripoid scallop by physical means
Owner:OCEAN UNIV OF CHINA
Method for inducing gynogenesis of Siberian sturgeon by using amur sturgeon sperm
InactiveCN101569293BIncrease the proportion of female individualsImprove farming efficiencyClimate change adaptationPisciculture and aquariaStart timeZoology
The invention relates to a method for inducing gynogenesis of a Siberian sturgeon by using an amur sturgeon sperm, which comprises the following steps: (1) inactivating a genetic material of the amur sturgeon sperm; (2) acquiring Siberian sturgeon unfertilized ova and performing insemination with an inactivated sperm of an amur sturgeon; (3) inducing the gynogenesis of the Siberian sturgeon through heat shock: inducing the gynogenesis of the Siberian sturgeon by adopting a method for inhibiting a second polar body from being discharged through the heat shock , wherein the starting time of theheat shock is 0.3 tau0 (18 minutes at a temperature of below 16 DEG C) after fecundation, the duration of the heat shock is 2 minutes, and the temperature of the heat shock is 37 DEG C; and (4) cultivating a gynogenesis induced egg. A super-female fish of the Siberian sturgeon cultivated by the technique of the method can produce gynoecy fish fries by mating with a normally cultivated milter, thusthe proportion of female individuals in cultured Siberian sturgeon population is improved, and the cultivation benefit is improved.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Method for producing triploid stichopus japonicus seedling
InactiveCN102100198ASuitable for industrial seedling productionInhibition releaseClimate change adaptationPisciculture and aquariaPlant Germ CellsSecond polar body
The invention provides a method for producing a triploid stichopus japonicus seedling, which comprises the steps of: firstly, enabling cultured mature stichopus japonicus to spawn and ejaculate, collecting ova together after the spawning is ended, adding sperms for carrying out artificial fertilization; when observing that 40-50 percent of germ cells release first polar bodies, adding a certain quantity of pure water in culture seawater with the germ cells to inhabit the release of second polar bodies, uniformly stirring, inducing triploid of the stichopus japonicas, re-transferring the induced germ cells to normal seawater for hatching; and finally, culturing the preferably selected stichopus japonicus triploid larva according to the conventional stichopus japonicus seedling-raising method. In the invention, the stichopus japonicus germ cells are treated by reducing the seawater salinity with the pure water to inhibit the release of the second polar bodies to generate the stichopus japonicus triploid, thus the method is simple in operation and is suitable for the industrialized seedling-raising production of the stichopus japonicus.
Owner:SHANDONG HAIYIBAO AQUATIC PROD
Method for inducing ocean seashell polyploidy by using sodium chloride
InactiveCN101658149BInhibition releaseEasy to operateClimate change adaptationPisciculture and aquariaPolar bodyCrassostrea rivularis
The invention discloses a method for inducing ocean seashell polyploidy by using sodium chloride. The method is characterized by comprising the following steps: selecting the seashell to grow the shell to respectively obtain sperms and ovum; carrying out artificial insemination; continuously observing the developing condition of zygotes through microscopic examination to determine the number of the water body for culturing the zygotes; adding sodium chloride into the culture sea water containing the zygotes according to a proportion that 20-40 go of sodium chloride is added in 1000ml of culture seat water when 40-50 percent of zygotes are observed to release a first polar body or a first zygote is observed to occur the first polar body, so as to prevent the release of a second polar body or the first polar body of the zygotes; evenly stirring and treating for 10-15 min; and transferring the zygotes to normal sea water to incubate . The ocean seashell comprises Pacific oyster, chlamys farreri, Argopecten irradians, Crassostrea rivularis, clam, Scapharca broughtonii. The seashell zygotes are treated by using the sodium chloride and the initiating agent so as to inhibit the release of the polar body and produce the shell polyploidy with no toxicity, efficiency, and simple operation.
Owner:山东安源种业科技有限公司
Method for in-vitro fertilization by taking testicular sertoli cells as trophoblasts
InactiveCN105647854ASimple systemImprove microenvironmentEmbryonic cellsGerm cellsTrophoblastic cellTrophoblast
The invention relates to a method for in-vitro fertilization by taking testicular sertoli cells as trophoblasts. The method includes the steps of (1), subjecting the testicular sertoli cells to isolated culture to prepare the sertoli cell trophoblasts; (2), guiding sperms into capacitation liquid containing well balanced HTF for incubation for 1 hour; (3), collecting ova; (4), transferring washed ova into a balanced culture medium with a testicular sertoli cell monolayer; (5), adding 20 microliters of incubated sperm suspension liquid into a culture solution containing the ova; (6), placing a culture dish back to an incubator with 5% CO2 at the temperature of 37 DEG C for incubation, and holding for 5-6 hours; (7), picking out the ova with second polar bodies and pronuclei and transferring into the culture medium for culturing. The method for in-vitro fertilization by taking the testicular sertoli cells as the trophoblasts has the advantages of high insemination rate and developmental rate.
Owner:QINGDAO AGRI UNIV
Method of breeding pearls by using 6-dimethylin purine to induce triploid jibheader clams
InactiveCN1957686BImprove survival rateThe induction effect is stableAnimal reproductionClimate change adaptationPurineZoology
A culture method for inducing triploid Hyriopsis cvmingii Lea by using 6-dimethylamino purine includes such steps as obtaining the ova of triploid Hyriopsis cvmingii Lea and fertilizing with sperm from diploid, using an inhibitor to suppress a first or a second polar body, which is characterized in that the inhibitor is 6-dimethylamino purine for short 6-DMAP. The invention also discloses a methof for culturing pearl by using the triploid Hyriopsis cvmingii Lea. The inhibitor of the invention has characteristics of low cost and toxicity, and adapts to culturing pearl in large water area.
Owner:HUNAN UNIV OF ARTS & SCI
A method for continuous batch induction of Atlantic salmon triploid
ActiveCN111194705BEasy to use in productionAs soon as possible to determine the induction effectClimate change adaptationPisciculture and aquariaAnimal scienceMedicine
The invention relates to chromosome manipulation technology, in particular to a method for continuous batch induction of Atlantic salmon triploid. Atlantic salmon sperm eggs are collected for artificial insemination, and the fertilized eggs are divided into 2-3 groups, and each group is successively subjected to hydrostatic pressure induction treatment to inhibit the discharge of its second polar body, thereby obtaining Atlantic salmon triploid; Pressure induction is, after fertilization 300-400 degrees (temperature ℃ × time min), the fertilized eggs of the first group are subjected to hydrostatic pressure treatment to obtain triploid Atlantic salmon; after the fertilized eggs of the first group are taken out, immediately The second group of fertilized eggs was induced by hydrostatic pressure according to the above process, so that the same batch of fertilized eggs could be continuously induced and treated. By adopting the method of the invention, 100% Atlantic salmon triploids can be continuously and efficiently induced.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
A kind of cultivation method of crucian carp gynogenetic fry
ActiveCN104904638BPure germplasmFast growthClimate change adaptationPisciculture and aquariaGenetic MaterialsCarp
The invention provides a cultivation method for carassius auratus gynogenesis fries. The method comprises the steps of selecting sexually mature diploid carassius auratus parents and collecting sperm and ovums; performing ultraviolet irradiation on Hanks liquid-diluted sperms to inactivate genetic materials thereof; performing artificial insemination on the inactivated sperms and the ovums; restraining the discharge of second polar bodies of meiosis by using a cold shock method to double genomes of the ovums and thus to cultivate viable carassius auratus gynogenesis diploid fries. Microsatellite marker identification proves that the genetic materials of all the gynogenesis fries are from the female parent; morphological measurement indicates part of the gynogenesis fries has obvious early growth dominance. The method has the advantages of simple operation, high efficiency, stability and high practicality.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Production method of triploid shellfishes
The invention relates to a production method of triploid shellfishes, in particular to a production method of the triploid shellfishes from diploid shellfish production to tetraploid mother shellfish production to triploid shellfish production. The production method is characterized by comprising the following steps of S1, a first stage of fertilization of diploid female eggs and diploid male sperms; S2, a second stage in which the release of a first polar body or a second polar body of fertilized eggs is prevented in the first stage S1 and triploid females are produced; S3, a third stage in which the female eggs in the second stage S2 are fertilized with the diploid male sperms; S4, a fourth stage in which the release of the first polar body is prevented from occurring from the eggs of the third stage S3 and tetraploid males are produced; and S5, the fifth stage in which the diploid female eggs are fertilized with the male sperms in the fourth stage S4, and the triploid shellfishes are generated.
Owner:李琪铎 +2
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