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Method for inducing seashells to generate polyploid

A polyploid and shellfish technology, applied in the field of inducing marine polyploid shellfish, can solve the problems of high price, poor induction effect, unstable induction effect, etc., and achieve the effect of simple operation

Inactive Publication Date: 2006-04-19
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because chemical reagents such as CB and 6-DMAP have strong toxicity, chemical induction has a certain poisonous effect on the embryos and operators of induced shellfish, and its price is expensive, so it is not easy to produce and apply; hydrostatic pressure in physical induction The treatment requires special instruments, and the induction efficiency is low and the induction effect is unstable. Although the temperature shock is relatively simple, the induction effect is poor; biological hybridization is simple and effective, but the acquisition of tetraploid broodstock is a difficult problem in the application of this method. bottleneck

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0006] Embodiment 1, first measure the salinity of the seawater used for cultivation with a salinity meter, take the Pacific oyster living in normal seawater (salinity is 30) as an example, get the Pacific oyster seed shell, and obtain the refined water respectively by dissecting or inducing discharge. , eggs, artificial fertilization, continuous microscopic examination to observe the development of fertilized eggs, when it is observed that 40-50% of fertilized eggs release the first polar body, in the low-salt range 4-16, wherein the salinity is 6-10 The induction effect is the best, and the oyster fertilized eggs are immediately hypotonically treated in low-salinity seawater with a salinity of 6-10 for 10-15 minutes to inhibit the release of the second polar body of the fertilized eggs and obtain a triploid rate of up to 89.5%.

Embodiment 2

[0007] Embodiment 2, first measure the salinity of the seawater used for cultivation with a salinity meter, take the Pacific oyster living in normal seawater (salinity is 30) as an example, get the Pacific oyster seed shell, and obtain the refined water respectively by dissecting or inducing discharge. , eggs, artificial fertilization, continuous microscopic examination to observe the development of fertilized eggs, when the first polar body is observed in the first fertilized egg, in the low-salinity range of 4-16, especially when the salinity is 6-10, induce The effect is good, and the oyster fertilized eggs are immediately hypotonically treated in low-salinity seawater with a salinity of 6-10 for 10-15 minutes to inhibit the release of the first polar body of the fertilized eggs and obtain a triploid rate of up to 82%.

[0008] The above-mentioned embodiments 1 and 2 are also applicable to other spawning marine shellfish such as scallops, abalones, clams, etc. The production...

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Abstract

A method for inducing the polyploid of marine shellfish includes such steps as measuring the salinity of culturing seawater, taking the sperms and ova of shellfish, artificial insemination, adding fresh water to seawater until its salinity is 4-16 when 40-50% of fertilized ova is releasing the first polar bodies, stirring for 10-15 min for suppressing the release of the second polar bodies, and incubating the fertilized ova in normal seawater.

Description

technical field [0001] The invention relates to a method for cultivating marine organisms, in particular to a method for inducing marine polyploid shellfish. Background technique [0002] Triploid shellfish has become an important part of shellfish farming due to its poor fertility, fast growth, and high quality. At present, there are three main methods for inducing polyploid shellfish: one is to use chemical inducers such as cytochalasin B (CB for short) or 6-DMAP to inhibit the release of polar bodies; the other is to use temperature shock or hydrostatic pressure physical induction; Is a biological hybrid, that is, the use of tetraploid and diploid hybrids to produce a full triploid. Because chemical reagents such as CB and 6-DMAP have strong toxicity, chemical induction has a certain toxic effect on the embryos and operators of induced shellfish, and its price is expensive, so it is not easy to produce and apply; The treatment requires special instruments, and the induc...

Claims

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Application Information

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IPC IPC(8): A61D19/00A61D19/02A01K61/00
CPCY02A40/81
Inventor 王昭萍于瑞海王如才施坤涛王艳
Owner OCEAN UNIV OF CHINA
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