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Method for inducing gynogenesis of Siberian sturgeon by using amur sturgeon sperm

A technique for gynogenesis and sturgeon sturgeon, which is applied in the field of gynogenesis of Siberian sturgeon induced by sturgeon sturgeon sperm, can solve problems such as unreported, and achieve the effects of improving breeding efficiency and increasing the proportion of female individuals

Inactive Publication Date: 2011-08-31
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

Foreign countries have also studied the techniques of homologous and heterologous sperm-induced gynogenesis in hybrid sturgeons (Hulus ♀×Acipenser corpus ♂), Russian sturgeon, Acipenser sinensis, Acipenser chinensis, Acipenser spp. The technique of inducing the gynogenesis of Siberian sturgeon by using the sperm of Sturgeon's sturgeon has not been reported at home and abroad

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  • Method for inducing gynogenesis of Siberian sturgeon by using amur sturgeon sperm
  • Method for inducing gynogenesis of Siberian sturgeon by using amur sturgeon sperm
  • Method for inducing gynogenesis of Siberian sturgeon by using amur sturgeon sperm

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Embodiment Construction

[0017] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0018] 1. Siberian sturgeon eggs and Sturgeons sperm

[0019] Three female Siberian sturgeon (weight 13.4-17.4kg) and three male sturgeon (weight 7.8-10.2kg) artificially bred by Hangzhou Qiandao Lake Sturgeon Science and Technology Development Co., Ltd. were artificially induced in March 2009 At 14:00 on March 3, the semen of Acipenser schrenckii was taken, the viability of microscopic examination was 95%, and the sperm was i...

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Abstract

The invention relates to a method for inducing gynogenesis of a Siberian sturgeon by using an amur sturgeon sperm, which comprises the following steps: (1) inactivating a genetic material of the amur sturgeon sperm; (2) acquiring Siberian sturgeon unfertilized ova and performing insemination with an inactivated sperm of an amur sturgeon; (3) inducing the gynogenesis of the Siberian sturgeon through heat shock: inducing the gynogenesis of the Siberian sturgeon by adopting a method for inhibiting a second polar body from being discharged through the heat shock , wherein the starting time of theheat shock is 0.3 tau0 (18 minutes at a temperature of below 16 DEG C) after fecundation, the duration of the heat shock is 2 minutes, and the temperature of the heat shock is 37 DEG C; and (4) cultivating a gynogenesis induced egg. A super-female fish of the Siberian sturgeon cultivated by the technique of the method can produce gynoecy fish fries by mating with a normally cultivated milter, thusthe proportion of female individuals in cultured Siberian sturgeon population is improved, and the cultivation benefit is improved.

Description

technical field [0001] The invention belongs to the field of gynogenesis induction of Siberian sturgeon, in particular to a method for inducing gynogenesis of Acipenser schrenckii sperm. Background technique [0002] Siberian sturgeon is the main economic aquaculture species in my country. It has the advantages of fast growth, strong adaptability, good meat quality, and high-quality caviar. Since it was first introduced into China in 1996, it has developed into a breeding output second only to Sturgeon's sturgeon. the second largest cultured species of sturgeon. Sturgeon caviar made from mature eggs of female sturgeon is known as "black gold" and is the product with the highest added value in sturgeon farming. The output value of each fish only producing caviar is 10,000 to 20,000 yuan, while male fish can only produce commercial fish or fish meat products, and the commodity price is only 30 to 40 yuan / kg, and the economic value is much lower than that of female fish. About...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张涛庄平章龙珍刘鉴毅赵峰冯广朋黄晓荣侯俊利闫文罡田美平颜世伟马境
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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