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Method for producing triploid stichopus japonicus seedling

A triploid and sea cucumber technology is applied in the field of producing triploid sea cucumber seedlings, which can solve the problems of long summer time, poor adaptability, and affecting the healthy and sustainable development of sea cucumber aquaculture.

Inactive Publication Date: 2011-06-22
SHANDONG HAIYIBAO AQUATIC PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Every year from March to late June is the breeding and development period of sea cucumbers. During this period, most of the energy used for growth is used for gonad development. After the breeding period, sea cucumbers enter a high temperature period and begin a long aestivation period. The high temperature tolerance is poor, and the summer time is long, generally about 100 days, which greatly prolongs the cultivation cycle of sea cucumbers. In addition, the sea cucumbers have poor physical fitness and poor adaptability to the environment at this time. Therefore, the death of sea cucumbers during this period The high rate affects the healthy and sustainable development of the sea cucumber aquaculture industry

Method used

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Embodiment Construction

[0006] A method for producing triploid sea cucumber seedlings is to dry the mature sea cucumber seedlings for 1 hour in the shade and stimulate them with running water for 0.5 hours, and then put the sea cucumbers one by one into different 0.03-0.05m 3 Add seawater to the aquarium and fiberglass bucket for spawning and fertilization. After spawning and fertilization, remove the female ginseng first, collect the eggs together, add sperm for artificial insemination, and observe the fertilization through continuous microscopic examination. For the development of eggs, when 40-50% of the fertilized eggs are observed to release the first polar body, add a certain amount of pure water to the cultured seawater with fertilized eggs to reduce the salinity to 10--15 to inhibit the second pole After 20-25 minutes of treatment, the fertilized eggs were relocated to normal seawater for incubation. By inhibiting the release of the second polar body of fertilized eggs, the triploid rate of A....

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Abstract

The invention provides a method for producing a triploid stichopus japonicus seedling, which comprises the steps of: firstly, enabling cultured mature stichopus japonicus to spawn and ejaculate, collecting ova together after the spawning is ended, adding sperms for carrying out artificial fertilization; when observing that 40-50 percent of germ cells release first polar bodies, adding a certain quantity of pure water in culture seawater with the germ cells to inhabit the release of second polar bodies, uniformly stirring, inducing triploid of the stichopus japonicas, re-transferring the induced germ cells to normal seawater for hatching; and finally, culturing the preferably selected stichopus japonicus triploid larva according to the conventional stichopus japonicus seedling-raising method. In the invention, the stichopus japonicus germ cells are treated by reducing the seawater salinity with the pure water to inhibit the release of the second polar bodies to generate the stichopus japonicus triploid, thus the method is simple in operation and is suitable for the industrialized seedling-raising production of the stichopus japonicus.

Description

technical field [0001] The invention relates to a method for raising sea cucumber seedlings, in particular to a method for producing triploid sea cucumber seedlings. Background technique [0002] As a precious seafood, sea cucumber is more and more popular due to its good nutritional value and health effects. In recent years, the farming scale of sea cucumber in the northern coast of my country has expanded rapidly. More than 400,000 mu, with an output value of more than 10 billion yuan, has become one of the advantageous industries in aquaculture in our province. But sea cucumber has the problems of slow growth rate and long breeding time. Every year from March to late June is the breeding and development period of sea cucumbers. During this period, most of the energy used for growth is used for gonad development. After the breeding period, sea cucumbers enter a high temperature period and begin a long aestivation period. The high temperature tolerance is poor, and the sum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 赵婷王虎成
Owner SHANDONG HAIYIBAO AQUATIC PROD
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