Method for producing triploid stichopus japonicus seedling
A triploid and sea cucumber technology is applied in the field of producing triploid sea cucumber seedlings, which can solve the problems of long summer time, poor adaptability, and affecting the healthy and sustainable development of sea cucumber aquaculture.
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[0006] A method for producing triploid sea cucumber seedlings is to dry the mature sea cucumber seedlings for 1 hour in the shade and stimulate them with running water for 0.5 hours, and then put the sea cucumbers one by one into different 0.03-0.05m 3 Add seawater to the aquarium and fiberglass bucket for spawning and fertilization. After spawning and fertilization, remove the female ginseng first, collect the eggs together, add sperm for artificial insemination, and observe the fertilization through continuous microscopic examination. For the development of eggs, when 40-50% of the fertilized eggs are observed to release the first polar body, add a certain amount of pure water to the cultured seawater with fertilized eggs to reduce the salinity to 10--15 to inhibit the second pole After 20-25 minutes of treatment, the fertilized eggs were relocated to normal seawater for incubation. By inhibiting the release of the second polar body of fertilized eggs, the triploid rate of A....
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