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Abalone triploid pharmaceutical induction method

A kind of technology of folded disc abalone and triploid, which is applied in the field of cultivating new varieties of folded disc abalone, and can solve the problems of unstable doubling rate and high embryo deformity rate

Inactive Publication Date: 2006-02-15
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the technical problems of unstable doubling rate and high embryonic deformity rate existing in the prior art, and provides a drug-induced wrinkled disc that treats wrinkled disc abalone with 6-dimethylaminopurine solution to inhibit the second polar body Abalone triploid method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] a. Dissolve 6-dimethylaminopurine (6-DMAP) produced by Sigma Corporation in the United States in distilled water or dimethyl sulfoxide, and store it at a temperature of -20 to 4°C for subsequent use;

[0015] b. Harvest seed abalone with a shell length of 8-12cm, no trauma, and soft parts that protrude obviously from the shell mouth. The effective accumulated temperature of the seed abalone for promoting ripening is above 1200°C. The sperm and eggs of the abalone are obtained by conventional methods and obtained by artificial insemination. fertilized egg;

[0016] c. Place the fertilized eggs in seawater with a temperature of 24-25°C. When the first polar body appears 30-50%, add 6-dimethylaminopurine solution to make the 6-dimethylaminopurine in seawater The final concentration is 75-150 μmol / l, the starting time is when 30-50% of the first polar body appears, and the duration is 15 minutes. After the second polar body is suppressed, the seed abalone is taken out;

[...

Embodiment 2

[0019] a. Dissolve 6-dimethylaminopurine (6-DMAP) produced by Sigma Corporation in the United States in distilled water or dimethyl sulfoxide, and store it at a temperature of -20 to 4°C for subsequent use;

[0020] b. Harvest seed abalone with a shell length of 8-12cm, no trauma, and soft parts that protrude obviously from the shell mouth. The effective accumulated temperature of the seed abalone for promoting ripening is above 1200°C. The sperm and eggs of the abalone are obtained by conventional methods and obtained by artificial insemination. fertilized egg;

[0021] c. Place the fertilized eggs in seawater with a temperature of 24-25°C. When the first polar body appears 30-50%, add 6-dimethylaminopurine solution to make the 6-dimethylaminopurine in seawater The final concentration is 75-150 μmol / l, the initial time is when the first polar body appears 30-50%, the duration is 20 minutes, and the seed abalone is taken out after inhibiting the second polar body;

[0022] c....

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Abstract

The invention discloses a method for inducing triploid of Haliotis discus Hannai through medicament, which comprises the following steps, preparing 6-dimethoxy aminopurine solution, obtaining abalone sperms and ova through the conventional method, obtaining oosperm through artificial insemination, placing oosperm of seed abalone into sea water, finally inhibiting the second polar body of the Haliotis discus Hannai with 6-dimethoxy aminopurine solution.

Description

Technical field: [0001] The invention relates to a method for cultivating a new variety of wrinkled abalone, in particular to a method for drug-induced triploid wrinkled abalone. Background technique: [0002] Abalone is a kind of marine shellfish, once known as one of the eight treasures of seafood. The wrinkle plate abalone (Haliotis discushannai) produced in Liaoning, Shandong and other places in my country, and the nine-hole abalone (H.diversicolor supertexta) in Fujian, Guangdong, Taiwan, Hainan and other places are the most famous, and its wrinkle plate abalone is called "soft gold". ", the price per ton in the international market reaches 40,000 to 60,000 US dollars. [0003] Our country started the research on the artificial breeding or breeding of wrinkled plate abalone from the 1970s, and carried out artificial breeding work in Liaoning, Shandong and other places in the 1980s, and achieved gratifying achievements. The national abalone annual output has reached mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N15/01
Inventor 王子臣
Owner DALIAN FISHERIES UNIVERSITY
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