Continuous batch induction method for salmo salar triploids

Abstract

The invention relates to chromosome operation technology, and particularly relates to a continuous batch induction method for salmo salar triploids. The method includes the following steps: collectingsalmo salar sperm eggs to carry out artificial insemination, and dividing fertilized eggs into 2-3 groups; and carrying out a hydrostatic pressure induction treatment on each group in a successive and continuous mode to inhibit emissions of second polar bodies, thereby obtaining the salmo salar triploids. According to the hydrostatic pressure induction treatment, 300-400 DEG C x minute (temperature x time) after insemination, the fertilized eggs in Group 1 is subjected to the hydrostatic pressure induction treatment to obtain the triploid salmo salar; after the induction, the fertilized eggsin the Group 1 are taken out, and then the fertilized eggs in Group 2 are immediately performed with the hydrostatic pressure induction according to the above-mentioned process, thus the same batch offertilized eggs can be induced and treated continuously. According to the invention, the method can be used for continuous and efficient induction to obtain 100% salmo salar triploids.

Description

technical field [0001] The invention relates to chromosome manipulation technology, in particular to a method for continuous batch induction of Atlantic salmon triploid. Background technique [0002] Atlantic salmon (Salmo salar), belonging to the order Salmoniformes and the family Salmonidae, commonly known as "salmon", is of high quality and belongs to high-end edible fish. With the development of my country's land-based industrial circulating water, deep-water cages, and farming workboats in recent years, as well as the continuously increasing market demand, the Atlantic salmon farming industry has gradually emerged. Under natural conditions, Atlantic salmon is generally sexually mature at the third age. During the process of sexual maturity, most of the energy is used for gonad development, resulting in slow growth and sharp decline in meat quality, thereby affecting its nutritional and economic value. In the process of artificial breeding, the phenomenon of precocious ...

AI Technical Summary

Problems solved by technology

Artificial induction of Atlantic salmon triploid has not been seen in China

It has been reported abroad in the 1980s that the pressure shock method can stably obtain triploids with a high induction rate, but its induction parameters are limited to several levels of treatment pressure and treatment time, and the survival rate is also low. Therefore, the optimal induction parameters or parameter ranges of Atlantic salmon triploid still need to be perfected

At the same time, the pressure shock method is limited by the volume of the pressure chamber, and the amount of fertilized eggs processed at one time is limited. Increasing the volume of the pressure chamber or increasing the number of presses will greatly increase the cost and difficulty of operation, which is unrealistic

If large-scale production is to be carried out, it can only be carried out by batch insemination and batch induction. The operation is cumbersome and the quality of fertilized eggs cannot be guaranteed, so the stability of the induction effect is poor

Often the same batch of fertilized eggs cannot be processed, and more triploids cannot be induced, resulting in a lot of waste and loss

So far, there have been no relevant technical reports on the same batch of fish eggs grouped and processed multiple times in a row at home and abroad

In addition, the hatching time of Atlantic salmon is long, and it takes more than 60 days to hatch. Most of the existing reports are for ploidy identification of hatched fry, which takes a long time to evaluate the effect of triploid induction, which brings inconvenience to its subsequent production management.

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