Trichoderma harzianum JSNL1404 and application thereof
A technology of microbial strains and bacterial agents, applied in applications, chemicals for biological control, biocides, etc., can solve problems such as root rot, economic loss, leaves, young shoots withering and dying, and achieve easy preservation and anti-corrosion. The effect of strong reverse ability and simple cultivation conditions
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Embodiment 1
[0040] The characteristics of embodiment 1 Trichoderma harzianum (Trichoderma harzianum) JSNL1404 bacterial strain
[0041] The morphology and physiological activity characteristics of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 described in the present invention are as follows:
[0042] Trichoderma harzianum (Trichoderma harzianum) JSNL1404 grows fast on the PDA medium plate of the PRX-250A type intelligent artificial climate box at 25 ± 1 ℃ (RH70%, dark), and the average daily growth is 2.7cm. White, fading to light green. The optimum temperature is 30°C, and it grows better at 35°C. Conidia are subspherical to ovoid, smooth surface, light green, 2.8-3.2×2.5-2.8μm in size.
[0043] The mycelia of Trichoderma harzianum JSNL1404 grew best at 25±1°C and pH 4-6, with an average daily growth of 2.6 cm; at 30°C, the sporulation was the highest at pH 6-8. The optimum growth temperature of the bacteria is 25±1℃.
[0044] The rDNA-ITS sequence of Trichoderma harzianum ...
Embodiment 2
[0046] The preparation method of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 inoculum: Inoculate Trichoderma harzianum JSNL1404 in liquid fermentation medium, pH 4.5, liquid volume in 500mL Erlenmeyer flask is 250mL, 25°C-30°C, shaker speed 120 revs / min, 72 hours, the fermented liquid that obtains is the original bacterium liquid of preventing and treating Dendrobium candidum candidum.
[0047] Wherein the formula of liquid fermentation medium is: 1000mL distilled water, 120g potato, 15g sucrose, 0.5g ammonium sulfate, pH4.5.
Embodiment 3
[0049] The preparation method of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 inoculum: inoculate Trichoderma harzianum JSNL1404 in liquid fermentation medium, pH 5.5, liquid volume in 500mL Erlenmeyer flask is 250mL, 25°C-30°C, shaker speed 160 revs / min, 120 hours, the fermented liquid that obtains is the original bacterium liquid of preventing and treating Dendrobium candidum candidum.
[0050] Wherein the formula of liquid fermentation medium is: 1000mL distilled water, 150g potato, 18g sucrose, 1.0g ammonium sulfate, pH5.5.
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