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Bacillus subtilis and application thereof

A technology of Bacillus subtilis and microbial strains, applied in the direction of application, bacteria, and fungicides, can solve the problems of antagonism mechanism and antagonistic substance analysis, and achieve the effects of easy industrial production, good development and application prospects, and easy preservation

Inactive Publication Date: 2011-08-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few analyzes on the mechanism of antagonism and antagonistic substances.

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0031] Experimental example 1: Bacillus subtilis (Bacillus subtilis) B37 on poplar discoloration fungus long beak shell [Ceratocystisadiposa (Butl.) C.Moreau, Hz91], cacao bisporus [Lasiodiplodia theobromae (Pat.) Griffon & Maubl., YM0737, Fx46 ], Fusarium (Fusarium sp., YM05)) plate antibacterial effect.

[0032] Inoculate the Bacillus subtilis (Bacillus subtilis) B37 strain activated on the LB slant by streaking on one side of the PDA plate, and then use a puncher with a diameter of 5mm to punch out the same quality and the same amount of bacterial blocks , click on the other side of the tablet. Cultivate at 28°C for 2 days to observe the presence or absence of the bacteriostatic zone, and record its width (as shown in Table 1). The experimental results are as follows: Figure 2a to Figure 2d shown. The test results showed that Bacillus subtilis (Bacillus subtilis) B37 had antagonistic effects on the four pathogenic bacteria.

[0033] Table 1B37 inhibits 4 species of popl...

experiment example 2

[0035] Experimental Example 2: Bacillus subtilis (Bacillus subtilis) B37 metabolites on poplar discoloration fungus [Ceratocystisadiposa (Butl.) C.Moreau, Hz91], cacao bisporus [Lasiodiplodia theobromae (Pat.) Griffon & Maubl., YM0737, Fx46] , Fusarium (Fusarium sp., YM05) plate antibacterial effect.

[0036] The fermented liquid of Bacillus subtilis (Bacillus subtilis) B37 cultivated for 3 days is filtered with a bacterial filter (the filter membrane aperture is 0.25 μm); the PDA plate culture of poplar discoloration fungus is punched with a hole puncher with a diameter of 5 mm to obtain homogeneous and equal amounts Bacterial blocks of the same quality and the same amount were punched on the other side of the PDA plate with a 5mm diameter puncher, and the bacterial blocks were taken out; the filtered Bacillus subtilis (Bacillus subtilis) B37 sterile filtrate was connected to 100 μl of the hole on the other side of the PDA plate, incubated at 28°C for 48 hours, and observed. ...

experiment example 3

[0039] Experimental Example 3: A test to verify whether Bacillus subtilis (Bacillus subtilis) B37 has an inhibitory effect on discoloration fungi on poplar chips.

[0040] 1. Experimental equipment: incubator, shaker, ultra-clean workbench;

[0041] 2. Experimental principle:

[0042] Soak the wood chip in the culture solution of Bacillus subtilis B37, take it out, and drain it. Bacillus subtilis B37 can use the medium sticking to the wood chip to continue to grow and produce metabolites, thereby inhibiting poplar. Growth of discoloration fungus.

[0043] 3. Experimental method:

[0044] 1) Cultivation of Bacillus subtilis (Bacillus subtilis) B37 bacterial solution: inoculate Bacillus subtilis (Bacillus subtilis) B37 into a Erlenmeyer flask containing 50 ml of LB medium, and culture at 35° C. for 60 hours at 120 rpm.

[0045] 2) Preparation of bacteria-carrying wood chips: soak sterilized wood chips with a size of 5×2 cm in cultured Bacillus subtilis B37 bacteria solution, ...

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Abstract

The invention discloses bacillus subtilis, which is named as Bacillus subtilis B37 in a classified manner and preserved with a preservation number of CGMCC.NO.4277 in a General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms on October 27th, 2010. The bacillus subtilis is mainly used for biological control of poplar chlorosis. The Bacillus subtilis B37 disclosed by the invention has an inhibiting effect on multiple stain fungi on the poplar; the antagonistic bacteria has the advantages of very good control effect on multiple plant diseases and pests, high propagation speed, manual culture availability, easiness for operation, convenience for production and application, strong resistance to adverse conditions and the like, and especially has the advantages of great significance on the control of the poplar chlorosis caused by the fungi, simple culturing condition, easiness for preservation, easiness for industrial production and good development and application prospects.

Description

technical field [0001] The invention belongs to the field of wood protection, in particular to a bacillus subtilis and its application in preventing and treating poplar discoloration disease. Background technique [0002] Poplar is one of the important fast-growing timber tree species in the world, and it is also the main fast-growing and high-yielding tree species in my country. It has the characteristics of wide adaptability and fast growth speed, and is welcomed by poplar growers. At present, the area of ​​poplar forests in my country has exceeded 8 million ha, and the stock volume has reached 429 million m 3 , ranking first in the world, surpassing the sum of the area of ​​poplar plantations in other countries, of which the area of ​​poplar timber forests is 3.09 million ha, accounting for about 40% of the national poplar plantation area. In East China, the poplar processing industry has become the local economy pillar. There are more than 600,000 mu (about 40,000 ha) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 赵桂华张小华席刚俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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