The invention belongs to the technical field of application of peony meal obtained after peony oil extraction, in particular to a comprehensive utilization method of peony meal. The method provided by the invention comprises the following steps: pretreating peony seed meal, then extracting the peony seed meal by supercritical carbon dioxide, and further extracting paeonol, peony saponin, peony protein and peony polysaccharide from the residue obtained after peony oil extraction, wherein paeonol, peony saponin, peony protein and peony polysaccharide can be used as food material or prepared into feed. The method provided by the invention has the beneficial effects that by adopting the method to process the peony meal, not only can peony oil be extracted from the peony meal, but also paeonol, peony saponin, peony protein and peony polysaccharide can be further extracted from the peony meal to be used as food material or prepared into feed, so that the economic value is improved, and the comprehensive utilization of the peony meal is realized.