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Culter alburnus gynogenesis inducing method

A technology of gynogenesis and gynogenesis of Cichlids, which is applied in the field of induction of gynogenesis of Cichlids, can solve the problems that the induction of gynogenesis of Cichlids has not been reported, and achieve easy identification, strong operability, and simplified identification procedures Effect

Active Publication Date: 2015-04-15
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

So far, there has been no report on the induction of gynogenesis in C.

Method used

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  • Culter alburnus gynogenesis inducing method
  • Culter alburnus gynogenesis inducing method
  • Culter alburnus gynogenesis inducing method

Examples

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Embodiment Construction

[0034] The method for inducing the gynogenesis of the crocodile chinensis of the present invention is specifically as follows:

[0035] It involves joint links such as enhanced parental cultivation, artificial oxytocin, inactivation of sperm genetic material, and doubling of fertilized egg chromosomes. The specific technical process and parameters are as follows:

[0036] 1. Strengthen cultivation. The primary condition for the success of this technical invention is to obtain the parents of the gonads of the Culper and carp with good gonad development. From December to January of the next year, select from the lakes or ponds more than 3 years old, no disease and no injury, and a body weight of more than 1.0kg. The improved species of Clamella is cultivated in a special pond, and the ratio of male to female is 2:1. During the cultivation period, high-quality extruded compound feed is fed at the beginning, and some fresh and live bait is added later, such as fresh and live sma...

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Abstract

The invention discloses a method for inducing the gynogenetic development of Cichlid carp, which comprises the following steps: (1) collecting carp semen; (2) carrying out ultraviolet genetic inactivation treatment on carp semen; Carp semen after ultraviolet genetic inactivation treatment was used for dry insemination; (4) Fertilized eggs were treated with cold shock and placed in hatching containers for hatching. Normal surviving fry were gynogenetic fish. The present invention adopts the ultraviolet genetic inactivation sperm of common carp to stimulate the eggs of the cymbidium, inhibits the release of the second polar body of the eggs by cold shock, and promotes chromosome doubling, and successfully obtains the gynogenetic individuals of the cymbidium. Since the normal surviving individuals are gynogenetic fish, there is no need for subsequent identification and analysis of chromosomes and genetic markers, which significantly simplifies the identification procedure.

Description

technical field [0001] The invention relates to fish biotechnology, in particular to a method for inducing the gynogenesis of the cichlid cichlid. Background technique [0002] Crawfish ( Culter alburnus Basilewsky) is one of the important freshwater economic fishes in my country. At present, most of the aquaculture Culturus still remain in the use of natural broodstock or multi-generation selected cultured species. Not only have their breeding traits not been explored, but also due to improper parent selection, inbreeding, etc., the breeding traits have appeared slow growth, Different degrees of germplasm decline such as meat quality decline. Artificial gynogenesis induction is an important technical means to quickly fix the traits of the female parent and sex control in fish, which can speed up the breeding process and efficiency of fish, especially fish with a long generation cycle. Studies have shown that one artificial gynogenesis induction is equivalent to 14 gener...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 顾志敏贾永义
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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