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Method for inducing ocean seashell polyploidy by using sodium chloride

A technology of sodium chloride and polyploidy, applied in application, climate change adaptation, fish farming, etc., can solve the problems of waste liquid polluting the environment, difficult production promotion and application, embryo and operator poisoning, etc.

Inactive Publication Date: 2010-03-03
山东安源种业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical inducers such as CB and 6-DMAP are mainly used in scientific research and production. However, due to the strong toxicity of chemical agents such as CB and 6-DMAP, chemical induction is harmful to the embryos and operators of shellfish. All have certain toxic effects, waste liquid pollutes the environment, and its price is expensive, and it is not easy to produce and popularize; in physical induction, hydrostatic treatment requires special instruments, and the amount of fertilized eggs to be processed is small, which is not suitable for production and application, and the induction efficiency Low, the induction effect is unstable, although temperature shock is relatively simple, but the induction effect is poor; biological hybridization is simple and effective, but the acquisition of tetraploid broodstock is a difficult bottleneck in the application of this method

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0006] Embodiment 1, taking Pacific oysters living in normal seawater (salinity is 30) as an example, take Pacific oyster seed shells, dissect or induce discharge, obtain sperm and eggs respectively, carry out artificial fertilization, and observe the fertilized eggs through continuous microscopy According to the development situation of the fertilized eggs, the number of water bodies for fertilized eggs is determined to be 3000ml. When it is observed that 40-50% of the fertilized eggs release the first polar body, add sodium chloride at the ratio of 1000ml of seawater for cultivation and 20-40 grams of sodium chloride. In the present embodiment, 30 grams of sodium chloride is added to every 1000 ml of seawater for cultivation, and the induction effect is good. Immediately add 90 grams of sodium chloride to the water body of Pacific oyster fertilized eggs to suppress the release of the second polar body of Pacific oyster fertilized eggs, and stir evenly. After 15 minutes of tre...

Embodiment 2

[0007] Embodiment 2, taking the scallop scallop living in normal seawater (salinity is 30) as an example, take the scallop scallop species, through induced discharge, obtain sperm and eggs respectively, carry out artificial fertilization, and observe the fertilized eggs by continuous microscopy According to the development situation of the fertilized eggs, the number of water bodies for cultivating fertilized eggs is determined to be 3000ml. When the first polar body is observed in the first fertilized eggs, 105 grams of seawater is added to the fertilized eggs of Chlamys farreri according to the ratio of 35 grams of seawater cultivated in 1000ml. Sodium chloride inhibits the release of the first polar body of the fertilized eggs of Chlamys farreri. Stir evenly. After 10 minutes of treatment, transfer the fertilized eggs to normal seawater for incubation. The highest triploid rate is 90%.

[0008] The foregoing embodiments 1 and 2 are also applicable to other spawning marine s...

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Abstract

The invention discloses a method for inducing ocean seashell polyploidy by using sodium chloride. The method is characterized by comprising the following steps: selecting the seashell to grow the shell to respectively obtain sperms and ovum; carrying out artificial insemination; continuously observing the developing condition of zygotes through microscopic examination to determine the number of the water body for culturing the zygotes; adding sodium chloride into the culture sea water containing the zygotes according to a proportion that 20-40 go of sodium chloride is added in 1000ml of culture seat water when 40-50 percent of zygotes are observed to release a first polar body or a first zygote is observed to occur the first polar body, so as to prevent the release of a second polar body or the first polar body of the zygotes; evenly stirring and treating for 10-15 min; and transferring the zygotes to normal sea water to incubate . The ocean seashell comprises Pacific oyster, chlamys farreri, Argopecten irradians, Crassostrea rivularis, clam, Scapharca broughtonii and the like. The seashell zygotes are treated by using the sodium chloride and the initiating agent so as to inhibit the release of the polar body and produce the shell polyploidy with no toxicity, efficiency, simple operation and extremely low cost.

Description

technical field [0001] The invention relates to a method for inducing polyploidy of marine shellfish, in particular to a method for inducing polyploidy of marine shellfish with sodium chloride. Background technique [0002] Triploid shellfish has become an important part of shellfish breeding due to its poor fertility, fast growth, and high quality. There are three main methods to induce polyploid molluscs: one is to use chemical inducers such as cytochalasin B (CB for short) or 6-DMAP to inhibit the release of polar bodies; the other is to use temperature shock or hydrostatic pressure physical induction; Is a biological hybrid, that is, the use of tetraploid and diploid hybrids to produce a full triploid. At present, chemical inducers such as CB and 6-DMAP are mainly used in scientific research and production. However, due to the strong toxicity of chemical agents such as CB and 6-DMAP, chemical induction is harmful to the embryos and operators of shellfish. All have cert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 于瑞海王昭萍邸炜鹏张晨晨
Owner 山东安源种业科技有限公司
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