118 results about "Polyploidies" patented technology

A method for producing polyploid plants of orchids includes the steps of: providing a protocorm or protocom-like body (PLB) of an orchid, the protocorm or PLB having an upper portion with a growing point and a lower portion without any growing point; cutting the protocorm or PLB approximately at a point of half height to separate the upper portion; subculturing the lower portion of the protocorm or PLB in an inducing medium, and putting a cut surface of the lower portion of the protocorm or PLB to face upward so that one or more next-generation PLBs grow from the cut surface of the lower portion. The method characterized in using no antimicrotubule agent can simplify the entire process of orchid polyploidy breeding, and can be used in mass-production of the stable polyploid plants.

The invention discloses a method for producing catharanthus roseus alkaloid. The method comprises the following steps: firstly, dedifferentiation culture is performed on the explant of the catharanthus roseus to get the callus of the catharanthus roseus, and through succesive transfer culture, loose and quick-growing cell line is obtained; secondly, the cell line of the callus of the catharanthus roseus obtained in the step one is processed through inducing the culture in basal culture medium provided with colchicines to get polyploidy cell line, and then expanding culture is performed to get a large amount of catharanthus roseus polyploidy cells; thirdly, the catharanthus roseus polyploidy cells are inoculated into a deffined medium to get cells being rich ofcatharanthus roseus secondary metabolites. The method of the invention produces the catharanthine with the catharanthus roseus, the industrial production of the catharanthine can be firmly realized, the production cost is low, the culture period is short, the production is not influenced by natural environment and climate, and the year-round production can be realized.

The invention discloses a method for cultivating triploid dendrobium huoshanense by a crossbreeding method of tetraploid plants and diploid plants. The method comprises the following steps: (1) dendrobium huoshanense species which are from different producing areas and have characteristics of good stress resistance, rapid growth, thick stalk, long stem, many tillers, low fiber content and high active ingredient content are preferably selected as polyploidy cultivated original plant parents; (2) seeds of the different species of original plants undergo aseptic sowing in a solid medium so as to obtain diploid protocorms; (3) protoplasts of the different species of diploid protocorms are prepared and cell fusion is carried out; (4) detected tetraploid plants undergo asexual tissue culture so as to obtain tetraploid plant seedlings; (5) the tetraploid plant seedlings undergo hardening-seedling acclimatization and cultivation to flowering; (6) the tetraploid plants and diploid plants undergo artificial pollination and crossbreeding, and fructification is completed to obtain triploid dendrobium huoshanense seeds; and (7) the triploid seeds undergo aseptic sowing and culture so as to obtain a lot of triploid seedlings.

The invention discloses a selecting and breeding method of a polyploidy novel mulberry tree breed. The breeding method comprises the following steps: implementing artificial hybridization and chromosome induction of polyploidy and other modes by selecting mulberry tree diploid hybridized mulberry seeds or hybridized seed mulberry sapling disease-free plants as materials; selecting out bud variation and branches the shape of which are similar to the polyploidy or plants by excellent plant, single plant selection and system selection; carrying out simple bud grafting on branch break bud variation the shape of which is similar to the polyploidy or plants in a primary selection garden; carrying out microscopical examination on the selected polyploidy single plants; establishing a system selection garden after determining that the single plants are polyploidy; and breeding a mulberry tree polyploidy novel breed by establishing product test gardens and parent gardens. The novel breed has extremely-high quality, ultrahigh yield and wide applicability; and in the actual production, a mulberry tree breed with ultrahigh yield matched with early growth, middle growth and late growth mulberry can be developed and bred, adapts to the requirement of pluralized and multiple silkworm breeding production all the year around on mulberry breeds and has obviously-improved mulberry yield, mulberry quality and other economic characters.

The invention relates to a query optimization method based on an improved genetic algorithm, and belongs to the technical field of query optimization. The method comprises: establishing a mathematical model for a query execution policy set, to transform a query optimization problem into a mathematical problem of finding a global optimal solution, wherein the model is called a cost evaluation model of a query policy; and then improving a genetic algorithm, and using a global search ability of the improved genetic algorithm to perform a parallel search on a query policy set, thereby finally obtaining an ideal query execution policy. According to the method provided by the invention, a conventional genetic algorithm is improved, and the improved genetic algorithm is used for query optimization of a large relational database, thereby overcoming a "premature" convergence phenomenon. Compared with other intelligent optimization algorithms, falling into a local extremum can be effectively avoided, thereby shortening search time. In addition, a gene based search policy and a polyploidy based retention policy in the algorithm greatly improve search accuracy.

The invention discloses a method for cultivating an aneuploid perennial forage grass variety by using a corn heterologous polyploidy and belongs to the field of corn distant hybridization. According to the method, MTF-1 (Tripsazea creammaize T.2n=76) cultivated by Sichuan Agricultural University is used as a female parent and teosinte is used as a male parent to be hybridized; a filial generation is selected from perennials, a wintering habit, a tiller number, a chromosome number and chromosome constitution to select the perennial heterologous aneuploid forage grass variety. According to the method, firstly, a heterologous aneuploid is used as the forage grass variety to be applied so as to creatively expand an application range of corn affinis species and explore a novel way for utilizing corn affinis specie materials; secondly, a material cultivated by the method is used as a bridge material and has a very great application value; furthermore, a vegetative propagation manner can be used for fixing a hybrid vigor and the variety is perennial, so that the production cost is very low, the yield and the nutritive value are high, and the economic benefit is great.

The invention discloses a sexual polyploidization breeding method for tetraploid petunias. The method comprises the following steps: firstly, determining the sizes and the morphological characteristics of flower buds at a reduction mitosis prophase I of diploid petunias to grasp the optimal treatment period; carrying out inducing treatment with colchicines with optimal concentration for proper times, observing the sizes and the morphological characteristics of petunia 2n pollen and n pollen of the petunias to rapidly distinguish and identify the 2n pollen; carrying out sexual polyploidization hybridizing; and identifying ploidies of hybridized F1 plant of the petunias by a simple and easy cytology identification method. With the adoption of the breeding method, the obtained sexual polyploidization hybridized F1 not only has the characteristics of large volume of a parent polyploidy organ, but also has the inheritable characters from a diploid male parent; compared with an autotetraploid obtained by an asexual polyploidization manner, the tetraploid petunias have wider genetic diversity, and higher ornamental value and seed setting rate.

The invention discloses a method for processing unibract fritillary bulb seeds. The method comprises the following steps of: physiologically and morphologically after-ripening the unibract fritillary bulb seeds in a shorter time by using gibberellin and combining a low-temperature sand storage stratification method; performing polyploid induction processing on the after-ripened unibract fritillary bulb seeds by using solution of colchicine; and culturing a new unibract fritillary bulb polyploidy variety with high active ingredient content. The method has the advantages of short processing time, high active ingredient content, high plant environment adaptability and the like.

The invention relates to a method for cultivating the novel variety of tetraploid dendrobium by use of the seed protocorm (PLBs). After the artificial pollination, the immature dendrobium seed in the development stage is carried out non-symbiotic sproution cultivation for 60 days in the 1 / 2MS liquid culture medium, which is then transferred into the 1 / 2MS liquid culture medium containing 0.02 percent Oryzalin for process and then transferred into the 1 / 2MS solid culture medium for continuous cultivation; after that, till the dendrobium seedling is shaped, the dendrobium is not planted, transplanted and managed by a conventional method until the dendrobium blooms. The invention overcomes the defect of generally low mutagenesis frequency of the polyploid and has better appreciation; moreover, the method is easy and practical, which increases the mutagenesis frequency (by over 60 percent) of the polyploidy in a large scale and provides enough polyploidy (over hundreds of plants) obtained one time to be used for the seed selection with high application value. Besides, the invention is applicable to the large-scale production with the advantages of small investment and high output.

The present technology generally relates to a method for inducing polyploidy in a Cannabis plant, the method comprising treating the Cannabis plant or a part thereof with an amount of a dinitroaniline compound effective to induce polyploidy.

The invention discloses a breeding method for resistant super-male polyploidy asparagus based on flow cytometry. The method comprises the following steps: (1) choosing excellent male plants in a field and carrying out indoor planting and microscopic examination; (2) mechanically separating microspores; (3) carrying out flow sorting; (4) carrying out suspension culture; (5) screening resistant mutants; (6) carrying out artificial doubling; (7) carrying out flow analysis; (8) subjecting the microspores obtained in step (7) respectively to field selective matching and cross combination and to in vitro rapid propagation; and (9) carrying out variety comparative tests on plants obtained after field selective matching and cross combination and plants obtained after in vitro rapid propagation, and applying plants obtained in the tests in production in the field. The method provided by the invention has the following beneficial effects: (1) a breeding period is shorter, breeding efficiency is higher, and operation is simpler; (2) an obtained variety has comprehensiveness; and (3) a breeding technology is novel.

The invention relates to a plant variety cultivating method, especially a method of cultivating lily polyploidy. 1. according to routine lily cultivating technique, selecting seed balls and jarovizing at low temperature to break dormancy to complete the jarovizing; 2. cultivating in a greenhouse or making field cultivation and management according to routine cultivating lily technique; 3. cultivating for 30-40days, then dripping 0.02-0.2% colchicines at 20 deg.C-25 deg.C; 4. according to the routine cultivating technique, processing the plants so that they will come into flower in 50-60 days, selecting the plants with extremely large stigmas and huge direct microscopic pollen particles to eliminate stamens so as to obtain 2n-oogamete containing stamens; 5. gathering normal pollens to hybridize with the obtained stamens, then according to routine cultivating technique, cultivating so that the fruits will swell fully in 45-60 days, thus able to gather them; 6. adopting routine triploid embryo in vitro culture method to be able to obtain the lily triploids.

The invention relates to the technology of plant tissue culture, and in particular relates to a tissue culture method for inducing populus yunnanensis dode tetraploid in combination with colchicine. The method taking petiole of populus yunnanensis dode as a tissue culture material in the process of tissue culture is characterized by comprising the following steps of: adding colchicine into a populus yunnanensis dode petiole differential medium to induce populus yunnanensis dode to generate polyploidy, wherein the treatment time is 20-30 days; carrying out differentiation culture again in a populus yunnanensis dode petiole differential medium in which colchicine is not added after the treatment; cutting the part which is obvious in variation on the differentiated cluster buds; repeatedly cultivating on the differential medium for 5-6 times, so that the populus yunnanensis dode petiole differential medium is stable in polyploidization variation; and performing rooting on the clump seedlings which are stably varied, so that the tissue culture seedlings of the tetraploid populus yunnanensis dode can be obtained. The method provided by the invention is the tissue culture method for inducing the populus yunnanensis dode tetraploid in combination with the colchicine, which is easy and simple to operate and can effectively reduce the chimera.

The invention discloses a fluorescence in situ hybridization (FISH) method for fish chromosomes. In the method, on the basis that a metaphase split phase specimen with a clear image and good chromosome spreading is obtained, a probe is marked by FISH technology and a nick translation method by taking Biotin-16-dUTP as a marker, a hybridization signal is amplified at two stages and a human 5.8S+28SrDNA probe is clearly positioned on a polyploid fish chromosome nucleolus organizer region (NOR). The chromosome ploidy and a karyotype of a polyploid are compared and analyzed by observing the chromosomal localization of ribosome 5.8S+28SrDNA on a polyploid fish, namely, the polyploid fish is proved to be a genetic polyploidy or an evolution polyploidy and an autopolyploid or an allopolyploid byanalyzing.

A cultivation method for mulberry chromosome doubling includes the following steps that firstly, stem tips of grafting seedlings of a target parent in the current year are subjected to continuous five-day instilling treatment by means of colchicines with the concentration gradient from 0.4% to 0.1%, wherein the grafting seedlings are 20-30 cm high; secondly, during treatment, kraft bags or envelopes are adopted for moisture retention and shading; thirdly, shoots germinating below treated portions are cleaned in time after treatment; fourthly, in the next year, abnormal leaves and branches are subjected to single-bud separation and grafting. The method has the advantage of being high in polyploidy induction rate. Experiments prove that the polyploidy induction rate can reach 32-56% by grafting individuals through the cultivation method for mulberry chromosome doubling, and the average polyploidy induction rate reaches 47% and is much higher than the domestic and international level.

The invention discloses a method for developing a marker through tetraploid potato high-throughput sequencing and an application of the method. The method for developing the marker through the tetraploid potato high-throughput sequencing disclosed by the invention can greatly reduce the complexity of a potato genome and can facilitate operation; and by virtue of the method, high-density SNP (single nucleotide polymorphism) sites can be rapidly identified, a chromosome label difference genetic interval for controlling target character can be obtained, and finally, the molecular marker can be developed on the basis of the difference genetic interval. With the application of the method disclosed by the invention, the application of an 2b-RAD sequencing technology in potato tetraploid material marker development is successfully achieved; the difficulty on developing the potato tetraploid material marker is solved, and meanwhile, a development cycle of the character closely linked marker is greatly shortened. In addition, the method disclosed by the invention also provides a reference for genetic interval mining, marker development and genome research of other tetraploid organisms having complex genomes.

The invention discloses a pickle and purple cabbage tri-genome species allohexaploid vegetable germplasm and an acquisition method, which fill in the blank of lack of brassica trigenomic species allohexaploid vegetable germplasm. The acquisition method comprises the steps that pickle and purple cabbages of brassica vegetable germplasm are cross-fertilized; an ovary is picked at the seventh day after pollination; mature seeds are obtained by embryo rescue; a large quantity of propagated progenies are obtained through subculture and propagation after seed germination; duplication treatment is carried out on a haploid by a liquid culture medium with colchicine to obtain the multi-genome allohexaploid vegetable germplasm. The pickle and purple cabbage tri-genome species allohexaploid vegetable germplasm and the acquisition method, disclosed by the invention, have the advantages of strong operability, simpleness and practicality, save the time for obtaining multi-genome polyploidy material, can be widely used for embryo rescue for interspecies cross of cruciferous brassica vegetable germplasm, and have great significance to cultivation and breeding of new species of special plants.

The invention belongs to the field of plant genetic breeding and provides a cultivation method for a polyploidy siraitia grosvenorii plant. The cultivation method for the polyploidy siraitia grosvenorii plant comprises the following steps: cultivating blades or stems of a siraitia grosvenorii female / male plant by applying an MS induced liquid culture medium to obtain siraitia grosvenorii clumpy shoot so as to be used for preparing the polyploidy siraitia grosvenorii plant, wherein epidermis cell scratch treatment is carried out on the blades or stems of the siraitia grosvenorii female / male plant before cultivation. The cultivation method for the polyploidy siraitia grosvenorii plant has the advantages that the polyploidy siraitia grosvenorii plant with excellent maternal inheritable characters can be rapidly and efficiently obtained, cost is low, efficiency is high, a variety is good, chromosome doubling of the siraitia grosvenorii female / male plant is realized, the obtained polyploidy siraitia grosvenorii plant is a prepared tetraploid usually and can be hybridized with a diploid siraitia grosvenorii male / female plant to obtain a triploid seedless variety, so that the cultivation method for the polyploidy siraitia grosvenorii plant has an important agricultural and medicinal guiding significance.

The invention relates to a polyploid induction method for Chinese gooseberry Hort 16A offspring fine strain tissue culture seedlings. The polyploid induction method comprises the following steps: soaking leaves of the Hort 16A offspring strain tissue culture seedlings in a colchicine water solution to induce polyploidy, washing the leaves, and inoculating in callus differentiation enrichment culture medium and subculture culture medium for light culture according to the steps. The polyploid induction method is simple in operation, good in induction directionality, high in induction success rate, low in cost, shorter in culture period and higher in induction rate, an efficient and feasible new method is provided for polyploidy breeding of Chinese gooseberry, the polyploid induction method has important application values, through the popularization of the polyploid breeding method, economic benefits can be brought for fruit farmers, and demands of more consumers are met.

The invention relates to a method for inducing a chill-proof Aglaonema spp polyploidy, relating to an Aglaonema spp breeding technology, which comprises the following steps of: inducing cluster buds, colchicine treating, carrying out low-temperature treatment, propagating variation plants, identifying the polyploidy, rooting and transplanting, wherein after being subjected to five generations of propagation, the variation plants are subjected to polyploidy detection, thus, the proportion of a chimera can be effectively reduced; the stems of the polyploidy plants are thickened and stunted in comparison with common stems, thus, the shapes are more attractive; the polyploidy plants have the chill-proof property and can resist 5 DEG C without cold injury; the temperature increasing cost of a greenhouse in winter is reduced; and the economical benefit is greatly increased.

The invention provides a method for obtaining polyploidy paulownia tomentosa by performing tissue culture propagation by taking young flowers as explants. The method comprises the steps of a, selecting the young flowers; b, pretreating and disinfecting the young flowers; c, performing callus induction on the young flowers; d, performing callus differentiation culture; e, differentiating seedlings to obtain roots; f, transplanting tissue culture seedlings; and g, observing and authenticating tissue culture plants. By the adoption of the young flowers serving as the explants, the method has the outstanding advantages of high regeneration capability and polyploidy tissue culture propagation in the period from winter to early spring; the problems that the root propagation rate of the polyploidy paulownia tomentosa is low and the leaf tissue culture propagation seedling differentiation frequency is low are solved; the polyploidy paulownia tomentosa can be propagated in a large scale; the authenticity and the consistency of polyploidy of the tissue culture offspring are determined by a morphology and cytology technology.

The invention discloses a pacific oyster no-damage sampling method, comprising the following steps of: a, firstly soaking pacific oyster for 10-15 hours with MgCl2 sea water solution with the concentration of 3-5%; b, then disinfecting a scalper or dissecting scissors with 70% alcohol, and scissoring pallium or gill of the pacific oyster; and c, washing the pacific oyster subjected to sampling operation in the steps a and b for 3-4 times with sea water, and putting the pacific oyster back into a breeding box for breeding. According to the method, a pacific oyster no-damage sampling method is established in a pacific oyster selective breeding process, an ideal parent can be provided for pacific oyster quality character family breeding, the method disclosed by the invention also can be applied to polyploidy breeding, and no-damage ploidy detection can be carried out on a polyploidy. High-quality varieties with good meat quality, fast growth speed and strong stress resistance are bred through selective breeding, or a triploid is obtained by adopting a polyploidy breeding technology, thus product quality of pacific oyster in China is improved.

The invention provides a cultivation technology for anoectochilus formosanus. The cultivation technology comprises the following steps: transplanting whole field diploid anoectochilus formosanus plants with soil into an artificial greenhouse, carrying out artificial pollination on the anoectochilus formosanus plants at the flowering phase, and picking after fruits ripen to obtain fruits of diploidanoectochilus formosanus; planting seed embryos of the diploid anoectochilus formosanus fruits into a germination culture medium for culturing to obtain protocorms, wherein the germination culture medium is prepared from MS, 0.9 to 1 mg / L of BA, 0.8 to 1 mg / L of KT, 0.08 to 0.12 mg / L of NAA, 25 to 30 mg / L of saccharose, 5 to 7 mg / L of agar and 20 to 22 mg / L of banana; carrying out dark culture onthe protocorms for 4 days and then soaking treatment in 8 to 10 [mu]mol / L of oryzalin solution for 10 to 36 hours; putting the soaked protocorms in a cluster culture medium for continuously culturingto obtain cluster buds, wherein the cluster culture medium is identical with the germination culture medium. Gravity progressive enhancement is adopted for stimulating in the rooting culture process,so that the development degree of root systems of polyploidy anoectochilus formosanus seedlings can be remarkably enhanced, and transplanting survival rate and rapid growth probability are improved.

The invention relates to the field of plant breeding and concretely relates to a centella asiatica polyploid and an inducing breeding method thereof. The inducing breeding method comprises transplanting wild centella asiatica to a medium, preparing an inducer solution containing 30-50mg / L of orizalina and 1-3wt% of dimethyl sulfoxide, wrapping centella asiatica fasciated leaf growth points through degreased cotton, soaking the degreased cotton with the inducer solution, carrying out treatment under natural illumination conditions for 2-4 days, carrying out detection through a chromosome microscope to determine a tetraploid sample and carrying out asexual expanding propagation and preservation. The method realizes centella asiatica in-vivo induction, has a high induction conversion rate and greatly improves medicinal effective substance content of the centella asiatica polyploidy.

The invention discloses an inducing method for improving the cell synchronization of tendril-leaved fritillary bulbs, which belongs to the field of biotechnologies. The method disclosed by the invention is mainly implemented by carrying out synchronized inducing on the mitosis of tendril-leaved fritillary bulbs by using a helium gas blocking method and low-temperature induction. The inducing method can effectively improve the follow-up polyploidy inductivity thereof, solve the problem of variety degeneration occurring in the process of planting tendril-leaved fritillary bulbs, can obviously shorten the planting cycle, and then improve the yield and medicinal component contents of tendril-leaved fritillary bulbs. The method is simple in steps, stable in process, and suitable for industrialized large-scale production.

The invention provides a technical flow applicable to the mass production of tetraploid radix scutellariae, which is characterized in that: radix scutellariae is used as a breeding material, a tetraploid radix scutellariae seedling with excellent and consistent characters can be rapidly obtained through the plant polyploidy breeding technology, the tissue culture and rapid propagation technology and the stem cuttage technology. The tetraploid radix scutellariae seedling belongs to the KT optimization, height of the tetraploid radix scutellariae seedling is 1.84 times of a reference, length of a leaf is 1.50 times of the reference, width of the leaf is 2.14 times of the reference, yield of a single plant is high, the yield of a single root is 1.70 times of a diploid reference, content of baicalin is improved by 1.86 percent compared to the reference, the fertility of the polyploidy radix scutellariea excellent variety is sterile, the entire growth period is 25 to 30 days longer than the reference, the tissue culture and rapid propagation technology is used for propagating the polyploidy radix scutellariea excellent variety to obtain original seedlings, and then the original seedlings are made into seedlings through the stem cuttage technology, the seedlings are transplanted to a field to be planted, about 5000 seedlings per Mu are planted, leaves and roots can be harvested in current year, about 40 to 50 kilograms of radix scutellariae tea can be produced per Mu, about 350 to 450 kilograms of roots can be produced, the content of the baicalin can reach 13.5 to 16.56 percent which is far higher than the requirement of pharmacopoeia that the content of the baicalin is not less than 8 percent, and the quality of the radix scutellariae is excellent.

The invention provides a method for culturing rhododendron fortunei polyploidy, and relates to the technical field of plant biology. The method comprises the following steps: sterilizing rhododendronfortunei seeds with oryzalin; transplanting the seeds to a seedling clod for culturing after the seeds are germinated, and detecting the ploidy variation of chromosome by a porosity method and a flowcytometer method; and transplanting the chromosome doubling plant determined by detection into greenhouse for culturing to obtain the polyploidy plant. The rhododendron fortunei polyploidy cultured byadopting the method has high inductivity reaching 30 percent, and high survival rate of variant plants reaching 85 percent or more; and the identifying method is simple and convenient, and can simultaneously prepare massive polyploidy materials, and the breeding efficiency is greatly improved.

Belonging to the technical field of plant polyploid breeding, the invention particularly discloses an efficient establishment method for a Chinese cymbidium sexual polyploidy. The method includes: selecting an efficiently occurring 2n gamete resources for hybridization, culturing hybrid seeds to form an intermediate propagule, observing the intermediate propagule, selecting quasi-like polyploidy intermediate propagule for differentiation to produce offspring seedlings, and identifying the offspring seedlings by morphological, flow cytometer and root tip chromosome tabletting so as to obtain the Chinese cymbidium sexual polyploidy. The method provided by the invention combines the plant ploidy advantage with heterosis, simultaneously establishes a technical system for efficient establishment of Chinese cymbidium sexual polyploid, and is of important significance for improving the core competitiveness and benefit of Chinese cymbidium industry in China, narrowing the gap of cymbidium breeding China and other countries as soon as possible, and promoting the efficient and sustainable development of flower industry.

The invention relates to a polyploidy cell breeding technique for red winged euonymus leaves, belongs to the field of plant ploidy breeding and relates to a method for cultivating a triploid plant by inducing the cells of red winged euonymus leaves. The method comprises the processes of adopting the endosperms of the red winged euonymus leaves for inducing callus and free protoplast and sorting the triploid protoplast under an axenic cultivation condition and inducing and cultivating the triploid protoplast into a triploid plant. The method further comprises the process of eliminating the apical bud dormancy of the triploid red winged euonymus leaves plant. According to the invention, the rich triploid cells are sorted from abundant diploid cells of diploid wild plant seed endosperms and cultivated into the triploid plant and the growth of the triploid plant is boosted.

The invention in particular relates to an induction method for hybrid orchid polyploidy belonging to the technical field of polyploidy breeding. The induction method for the hybrid orchid polyploidy comprises the following steps: taking sterile spherical stems formed by germination of hybrid seeds of Cymbidium Lucky Gloria 'Aguri' and Cymbidiumhybridium*(Cymbidiumtracyanumvarhuanghua*Cymbidiummastersii as test materials, carrying out polyploidy induction on F1-generation spherical stems by colchicines, then differentiating the induced spherical stems into buds and cluster buds, then carrying out propagation culture, carrying out rooting culture on plants which are identified as polyploidy to obtain polyploidy plant tissue culture seedlings. The hybrid orchid polyploidy plants obtained by combination of the spherical stems and a colchicines soaking method have remarkable polyploidy characteristics; the spherical stems are used as the induction materials, the period of breeding the polyploidy hybrid orchid plants is shortened; and the method is simple and easy to control.