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Cultivation method for mulberry chromosome doubling

A chromosome doubling and breeding method technology, applied in the fields of botany equipment and methods, plant genetic improvement, horticulture, etc., can solve the problems of separation, the lack of timely mutation of organs, the physiological toxicity of colchicine, etc., and achieve the polyploid induction rate. high effect

Inactive Publication Date: 2016-10-12
NORTHWEST A & F UNIV
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AI Technical Summary

Problems solved by technology

One of the main reasons is that colchicine is physiologically toxic, and improper handling results in the death of the treated individual; the second is that the action time of the drug is not enough; the third is that the mutant organs are not separated from the mother in time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] (1) Use 0.4%, 0.3%, 0.2%, and 0.1% colchicine with a concentration gradient from high to low to instill the shoot tips of the target parent's grafted seedlings for 5 consecutive days. 20~30cm;

[0012] (2) Use kraft paper bags or envelopes for moisturizing and shading during processing;

[0013] (3) After treatment, clean up the anvil buds sprouting below the treatment site in time;

[0014] (4) In the following year, single buds were separated and grafted on the branch segments of the abnormal leaves.

[0015] Experiments have proved that the chromosome doubling rate obtained by the grafted individual in this embodiment can reach 32-56%, with an average of 47%.

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Abstract

A cultivation method for mulberry chromosome doubling includes the following steps that firstly, stem tips of grafting seedlings of a target parent in the current year are subjected to continuous five-day instilling treatment by means of colchicines with the concentration gradient from 0.4% to 0.1%, wherein the grafting seedlings are 20-30 cm high; secondly, during treatment, kraft bags or envelopes are adopted for moisture retention and shading; thirdly, shoots germinating below treated portions are cleaned in time after treatment; fourthly, in the next year, abnormal leaves and branches are subjected to single-bud separation and grafting. The method has the advantage of being high in polyploidy induction rate. Experiments prove that the polyploidy induction rate can reach 32-56% by grafting individuals through the cultivation method for mulberry chromosome doubling, and the average polyploidy induction rate reaches 47% and is much higher than the domestic and international level.

Description

Technical field [0001] The invention relates to a method for cultivating mulberry artificial polyploid, in particular to a method for cultivating mulberry chromosome doubling. Background technique [0002] Mulberry polyploid has the advantages of vigorous growth, excellent leaf quality, and enhanced stress resistance. It is the main direction of mulberry breeding. At present, the common method for cultivating artificial polyploid mulberry is to use colchicine as a mutagen to treat mulberry seeds or growth buds. However, for a long time, the polyploid mutagenesis rate (the success rate of obtaining polyploidy) has been below 10% . One of the main reasons is that colchicine is physiologically toxic, and improper handling causes death of the treated individual; the second is the lack of time for the drug; and the third is that the mutant organs are not separated from the mother in time. Summary of the invention [0003] The technical problem to be solved by the present invention is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01G1/06
CPCA01H1/08A01G2/30
Inventor 苏超焦锋陈旗钱永华薛忠民肖乃康
Owner NORTHWEST A & F UNIV
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