35 results about "Oryzalin" patented technology

The invention provides a cultivation technology for anoectochilus formosanus. The cultivation technology comprises the following steps: transplanting whole field diploid anoectochilus formosanus plants with soil into an artificial greenhouse, carrying out artificial pollination on the anoectochilus formosanus plants at the flowering phase, and picking after fruits ripen to obtain fruits of diploidanoectochilus formosanus; planting seed embryos of the diploid anoectochilus formosanus fruits into a germination culture medium for culturing to obtain protocorms, wherein the germination culture medium is prepared from MS, 0.9 to 1 mg / L of BA, 0.8 to 1 mg / L of KT, 0.08 to 0.12 mg / L of NAA, 25 to 30 mg / L of saccharose, 5 to 7 mg / L of agar and 20 to 22 mg / L of banana; carrying out dark culture onthe protocorms for 4 days and then soaking treatment in 8 to 10 [mu]mol / L of oryzalin solution for 10 to 36 hours; putting the soaked protocorms in a cluster culture medium for continuously culturingto obtain cluster buds, wherein the cluster culture medium is identical with the germination culture medium. Gravity progressive enhancement is adopted for stimulating in the rooting culture process,so that the development degree of root systems of polyploidy anoectochilus formosanus seedlings can be remarkably enhanced, and transplanting survival rate and rapid growth probability are improved.

The invention discloses a method for rapidly obtaining herba erigernotis triploid. The method comprises the following steps: processing plump herba erigernotis seeds picked in the same year with a water solution of a cell microtubule inhibitor Oryzalin to induce the chromosome doubling of the germ growing point cells; continuously carrying out two times of leaf air hole identification in the seedling stage and the squaring stage, carrying out rapid screening so as to obtain tetraploid variant plants; and hybridizing the tetraploid variant plants with diploid plants (namely fusing egg cells having doubled chromosome with normal sperm cells) so as to obtain ploidy-homozygous triploid. The method utilizes the fusion of single cells (egg cells) with single cells (sperm cells) during the fertilization process of sexual reproduction of angiosperm, simultaneously achieves the ploidy purification of plant chromosome and obtaining of triploid, avoids a large amount of tedious work on purifying the ploidy of the tetraploid variant plants, and provides technical support for cultivating abundant herba erigernotis triploid novel germplasm and breeding triploid novel species.