35 results about "Oryzalin" patented technology

Dinitroaniline compounds useful for the treatment of diseases caused by parasitic protozoa in subjects in need of such treatment. The compounds are particularly useful in the treatment of leishmaniasis. The compounds are preferably less cytotoxic to normal cells than oryzalin. Also provided are methods of treating subjects having diseases caused by parasitic protozoa, preferably humans. The method comprising administering a therapeutically effective amount of a dinitroaniline compound of the present invention to a subject in need of such treatment

The present invention provides a breeding method for tetraploid ricinus communis. The method comprises: collecting a ricinus communis germplasm resource, performing morphologic character analysis and chromosome ploidy identification, selecting good diploid varieties, carrying out mutagenesis by using colchicine and oryzalin; performing chromosome ploidy identification on F1 generation seeds, selecting homozygous tetraploids, and performing economic character analysis and identification on the F2 generation, so as to breed good tetraploid ricinus communis.

The invention discloses a culturing method capable of effectively reducing the reverse mutation rate of horseradish polyploid plants. The method comprises the following steps: disinfecting horseradish seeds and sequentially culturing by a mixed solution of 5-aminuracil and propylene glycol, a mixed solution of oryzalin and propyzamide, and a mixed solution of colchicine and fumiron. With the culturing method, the reverse mutation rate of polyploid seedlings cultured by obtained polyploid seeds can be effectively reduced; the culturing method is a new way for rapidly culturing good horseradish varieties.

The invention provides a cultivation technology for anoectochilus formosanus. The cultivation technology comprises the following steps: transplanting whole field diploid anoectochilus formosanus plants with soil into an artificial greenhouse, carrying out artificial pollination on the anoectochilus formosanus plants at the flowering phase, and picking after fruits ripen to obtain fruits of diploidanoectochilus formosanus; planting seed embryos of the diploid anoectochilus formosanus fruits into a germination culture medium for culturing to obtain protocorms, wherein the germination culture medium is prepared from MS, 0.9 to 1 mg / L of BA, 0.8 to 1 mg / L of KT, 0.08 to 0.12 mg / L of NAA, 25 to 30 mg / L of saccharose, 5 to 7 mg / L of agar and 20 to 22 mg / L of banana; carrying out dark culture onthe protocorms for 4 days and then soaking treatment in 8 to 10 [mu]mol / L of oryzalin solution for 10 to 36 hours; putting the soaked protocorms in a cluster culture medium for continuously culturingto obtain cluster buds, wherein the cluster culture medium is identical with the germination culture medium. Gravity progressive enhancement is adopted for stimulating in the rooting culture process,so that the development degree of root systems of polyploidy anoectochilus formosanus seedlings can be remarkably enhanced, and transplanting survival rate and rapid growth probability are improved.

The invention discloses an induction method of Dendrobium polyploidy, comprising the steps of using 60-90 mu M of Oryzalin to soak pollinated Dendrobium megasporocytes and Dendrobium capsules in meiosis phase continuously for 48-72 hours, washing with clear water to remove Oryzalin, aseptically germinating seeds, identifying chromosome, and acquiring a polyploid plant from offspring. The chimera problem of Dendrobium polyploidy is solved, the problem that conventional triploid cultivation takes long time and is complex to operate is solved, and a triploid can be successfully cultivated conveniently within one growth period.

The invention discloses a method for rapidly obtaining herba erigernotis triploid. The method comprises the following steps: processing plump herba erigernotis seeds picked in the same year with a water solution of a cell microtubule inhibitor Oryzalin to induce the chromosome doubling of the germ growing point cells; continuously carrying out two times of leaf air hole identification in the seedling stage and the squaring stage, carrying out rapid screening so as to obtain tetraploid variant plants; and hybridizing the tetraploid variant plants with diploid plants (namely fusing egg cells having doubled chromosome with normal sperm cells) so as to obtain ploidy-homozygous triploid. The method utilizes the fusion of single cells (egg cells) with single cells (sperm cells) during the fertilization process of sexual reproduction of angiosperm, simultaneously achieves the ploidy purification of plant chromosome and obtaining of triploid, avoids a large amount of tedious work on purifying the ploidy of the tetraploid variant plants, and provides technical support for cultivating abundant herba erigernotis triploid novel germplasm and breeding triploid novel species.