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Breeding method of tetraploid non-heading Chinese cabbage

A technique for head cabbage and a cultivation method, applied in the field of genetics and breeding, can solve problems such as low water solubility, and achieve the effects of improving stability and inhibiting the formation of spindle filaments

Active Publication Date: 2018-09-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the direct use of amisulfuryl has low water solubility and it is difficult to stay on the leaves

Method used

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  • Breeding method of tetraploid non-heading Chinese cabbage
  • Breeding method of tetraploid non-heading Chinese cabbage
  • Breeding method of tetraploid non-heading Chinese cabbage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Step 1: Select 216 plump seeds of non-heading Chinese cabbage harvested in the same year.

[0026] Step 2, the seeds are subjected to germination treatment, and the seeds are dialed into plug trays after being white. When the cotyledons of the seedlings are fully expanded, the mixed aqueous solution of amisulfuryl (concentration of amisulfuryl is 0.06mmol·L -1 , the concentration of Tween 80 is 0.02% v / v, and the concentration of Silwet L-77 is 0.02% v / v) in the morning and evening at the growth point of the seedlings, and the treatment is done 5 times.

[0027] Step 3, after the adult plants are grown, the tetraploid plants are first screened according to the length and width of the 5th to 10th true leaves, the degree of twisting of the leaves, the thickness of the main stem, and the plant height. Through the identification of stomata in anatomy, the tetraploid variation primary plants were determined, and the variation rate reached 4.63%.

[0028] Step 4: Enter the ...

Embodiment 2

[0043] Step 1: Select 212 plump seeds of non-heading Chinese cabbage harvested in the same year.

[0044] Step 2, the seeds are subjected to germination treatment, and the seeds are dialed into plug trays after being white. When the cotyledons of the seedlings are fully unfolded, the mixed aqueous solution of amisulfuryl (the concentration of amisulfuryl is 0.08mmol·L -1 , the concentration of Tween 80 is 0.02% v / v, and the concentration of Silwet L-77 is 0.02% v / v) in the morning and evening at the growth point of the seedlings, and the treatment is done 5 times.

[0045] Step 3, after the adult plants are grown, the tetraploid plants are first screened according to the length and width of the 5th to 10th true leaves, the degree of twisting of the leaves, the thickness of the main stem, and the plant height. Then through the identification of stomata in anatomy, the primary plants for tetraploid variation were determined, and the variation rate reached 9.91%.

[0046] Step ...

Embodiment 3

[0056] Step 1: Select 199 plump seeds of non-heading Chinese cabbage harvested in the same year.

[0057] Step 2, the seeds are subjected to germination treatment, and the seeds are dialed into plug trays after being white. When the cotyledons of the seedlings are fully unfolded, the mixed aqueous solution of amisulfuryl (the concentration of amisulfuryl is 0.1mmol·L -1 , the concentration of Tween 80 is 0.02% v / v, and the concentration of Silwet L-77 is 0.02% v / v) in the morning and evening at the growth point of the seedlings, and the treatment is done 5 times.

[0058] Step 3, after the adult plants are grown, the tetraploid plants are first screened according to the length and width of the 5th to 10th true leaves, the degree of twisting of the leaves, the thickness of the main stem, and the plant height. Through the identification of stomata in anatomy, the tetraploid variation primary plants were determined, and the variation rate reached 8.04%.

[0059] Step 4: Enterin...

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Abstract

The invention discloses a breeding method of a tetraploid non-heading Chinese cabbage. According to the breeding method, a cell microtubule inhibitor oryzalin mixed water solution is dropwise added onthe growth points of seedlings of a non-heading Chinese cabbage so as to double the cell chromosomes; in the seedling period, pore identification is performed, in the blossoming period, pollen identification is carried out, and finally tetraploid plants are screened out through the identification of flow cytometer. At the same time, tween 80 and Silwet L-77 are added into the oryzalin water solution; the tween 80 can increase the solubility of oryzalin and the stability of the oryzalin water solution, the Silwet L-77 can greatly reduced the water surface tension, thus the solution can be welladhered on the surface of leaves, and the tetraploid induction rate is increased. According to the method, the oryzalin mixed water solution is used to inhibit the formation of spindle fibers in thelater period of cell mitosis, thus chromosome doubling is realized, and technical support is provided for creating abundant novel tetraploid seeds of non-heading Chinese cabbage and novel tetraploid species.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding, and in particular relates to a method for cultivating tetraploid non-heading Chinese cabbage. Background technique [0002] Non-heading cabbage ( Brassica cappestris ssp. chinensis ), also known as Chinese cabbage, is a plant of the genus Brassica in the family Brassicaceae, and is a one-year or two-year-old herb. The plants are relatively short, with a shallow root system and well-developed fibrous roots. Non-heading cabbage originated in my country and is rich in minerals and vitamins. Through ploidy breeding, the plant genome can be polyploidized to obtain varieties with vigorous growth potential, strong adaptability and good stress resistance. Tetraploid refers to the somatic cells containing four chromosome groups. After artificial induction, selection and cultivation, tetraploid plants with high quality, high yield and high resistance can be obtained. [0003] As a substitut...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 刘同坤陈丽邵帅旭王红尧张蜀宁李英侯喜林
Owner NANJING AGRICULTURAL UNIVERSITY
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