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Method for in-vitro inducing tetraploid watermelon by utilizing Oryzalin herbicide

A tetraploid and herbicide technology, applied in the field of watermelon tetraploid induction, can solve the problems of production limitation and low tetraploid production efficiency, and achieve the effect of overcoming the low frequency of induction

Inactive Publication Date: 2006-09-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Triploid watermelons are obtained through the hybridization of conventional diploids and tetraploids. The production of triploid watermelons is limited by many factors, one of which is the low production efficiency of tetraploids.
[0003] So far, there has been no invention of using the herbicide "Olaling" to efficiently induce watermelon tetraploids in vitro

Method used

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  • Method for in-vitro inducing tetraploid watermelon by utilizing Oryzalin herbicide
  • Method for in-vitro inducing tetraploid watermelon by utilizing Oryzalin herbicide
  • Method for in-vitro inducing tetraploid watermelon by utilizing Oryzalin herbicide

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Experimental program
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Embodiment 1

[0021] Embodiment 1, utilize the method for in vitro induction of watermelon tetraploid by Olarin herbicide, carry out the following steps successively:

[0022] 1), making explants:

[0023] Soak plump watermelon seeds in distilled water for 6 hours, carefully remove the shells, sterilize with 10% sodium hypochlorite solution for 10 minutes, shake continuously during the period, rinse repeatedly with sterile water, inoculate them on MS germination medium, and place them in the incubator for 28 Cultivate in the dark at ℃, and transfer to the cultivation room for cultivation when the radicle is exposed to 2-3mm. Then select 4 days seedling age cotyledon near hypocotyl end 5 * 5mm piece (band 1mm hypocotyl) as explant, the shape of this explant is as follows figure 1 shown. The MS germination medium used was prepared by adding 20 grams of sucrose and 7 grams of agar per liter of MS basic medium, and adjusting the pH value to 5.8.

[0024] 2), the above-mentioned explants were...

Embodiment 2

[0028] Embodiment 2, carry out the identification of tetraploid to above-mentioned watermelon regeneration plant:

[0029] From 8:00 to 9:00 in the morning, 1 to 2 cm of young root tips of the above-mentioned watermelon regenerated plants were taken as materials, treated with 0.002M 8-hydroxyquinoline at 20°C for 2 hours, washed with ethanol, ethanol:glacial acetic acid (volume ratio 3:1 ) fixed solution at 20°C for 12 hours, washed 2-3 times with pure alcohol until the material does not smell of glacial acetic acid, then washed with distilled water, kept at 45°C with 1mol / L hydrochloric acid for 30 minutes, washed with distilled water, Carbopin Red staining, placed under a microscope for observation, 30 cells with clear chromosomes were taken for chromosome counting, and the tetraploid chromosome identification picture is as follows Figure 5 shown. The conclusion is: adopting the method of the present invention can obtain tetraploid watermelon regenerated plants with high f...

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Abstract

The invention discloses a method of isolated inducing watermelon tetraploid using Ou laling herbicide, comprising the following steps: (1) taking cotyledon near hypocotyls of four-day life as explant; (2) pre-culturing the explant in enrichment culture medium for 4.5-5.5 days; (3) immersing the pre-cultured explant in liquid inductive culture medium, shocking in thermostatic oscillator and then treating under dark conditions for 11-13 hours; (4) washing the treated explant with sterilized water, replanting it to enrichment culture medium for 25 days and getting regenerating bud; (5) root culturing the regenerative bud, then training and replanting it to greenhouse and getting the watermelon regenerative plant with tetraploid of high frequency.

Description

technical field [0001] The invention relates to a watermelon tetraploid induction technology, in particular to a method for inducing watermelon tetraploid in vitro by using oraline herbicide. Background technique [0002] Watermelon is one of the top ten fruits in the world and is loved by consumers for its juiciness and strong flavor. Watermelon has a large area of ​​cultivation and is widely cultivated in tropical and subtropical regions; however, the genetic background of watermelon germplasm resources is relatively narrow, so it is necessary to use new techniques to obtain more germplasm resources. Triploid seedless watermelon is favored by consumers due to its good fruit quality and has great commercial potential. Triploid watermelons are obtained by crossing conventional diploids and tetraploids. The production of triploid watermelons is limited by many factors, one of which is the low production efficiency of tetraploids. The traditional method to obtain tetraploid ...

Claims

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Application Information

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IPC IPC(8): A01N33/06A01P21/00
Inventor 张明方张全美杨景华齐晓花
Owner ZHEJIANG UNIV
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